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Complex for rapidly detecting sulfide ions, and quantitative analysis method and applications thereof

A quantitative analysis and complex technology, applied in analytical materials, measuring devices, material analysis by observing the effect on chemical indicators, etc. "Natural eye" detection, good water solubility and biocompatibility, ultra-high selectivity

Active Publication Date: 2017-07-18
襄阳市大学科技园发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as a sulfur ion fluorescence detector, this compound has a detection limit of 700nM, which has certain limitations for trace detection; moreover, this compound cannot be successfully applied to the fluorescence imaging analysis of intracellular sulfur ion, so this document only examines the traditional Fluorescence Spectral Response of Sensitive System to Sulfide Ions

Method used

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  • Complex for rapidly detecting sulfide ions, and quantitative analysis method and applications thereof
  • Complex for rapidly detecting sulfide ions, and quantitative analysis method and applications thereof
  • Complex for rapidly detecting sulfide ions, and quantitative analysis method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A complex for rapid detection of sulfide ions and its quantitative analysis and application uses a coumarin derivative-copper ion complex as a sulfide ion fluorescence sensor to detect sulfide ions in a solution. The method mainly includes the following steps:

[0040] 1) Prepare a HEPES buffer solution with a concentration of 10 mM and a pH of 7.4; prepare a DMSO solution of compound C1 with a concentration of 2×10 -5 mol / L. Take 1mL of the DMSO solution of C1 above and place it in a 10mL colorimetric tube, and dilute to volume with HEPES buffer solution to obtain a concentration of 2×10 -6 mol / L, C1 solution with HEPES-DMSO volume ratio of 9:1 as solvent.

[0041] Then, in the solution of the above compound C1, a concentration of 1×10 -2 mol / L Cu(NO 3 ) 2 ·3H 2 O aqueous solution 5 μL (negligible effect on the total volume), to obtain a solution of the complex C1-Cu.

[0042] 2) Prepare a concentration of 3.0×10 with deionized water -3 mol / L Na 2 S aqueous so...

Embodiment 2

[0048] The solution of complex C1-Cu of the present invention is to S 2- The ultraviolet-visible absorption spectrometry titration, comprises the steps:

[0049] 1) Prepare a HEPES buffer solution with a concentration of 10 mM and a pH of 7.4; prepare a DMSO solution of compound C1 with a concentration of 7×10 -5 mol / L. Take 1mL of the DMSO solution of C1 above and place it in a 10mL colorimetric tube, dilute to volume with HEPES buffer solution to obtain a concentration of 7×10 -6 mol / L, C1 solution with HEPES-DMSO volume ratio of 9:1 as solvent.

[0050] Then, in the solution of the above compound C1, a concentration of 2×10 -2 mol / L Cu(NO 3 ) 2 ·3H 2 O aqueous solution 5 μL (negligible effect on the total volume), to obtain a solution of the complex C1-Cu.

[0051] 2) Prepare a concentration of 6.0×10 with deionized water -3 mol / L Na 2 S aqueous solution.

[0052] 3) Take 3 mL of the complex C1-Cu solution prepared in step 1) and place it in a quartz cuvette, and ...

Embodiment 3

[0055] The spectral response of the solution of the compound C1-Cu of the present invention to common anions mainly comprises the following steps:

[0056] 1) Prepare a HEPES buffer solution with a concentration of 10 mM and a pH of 7.4; prepare a DMSO solution of compound C1 with a concentration of 2×10 -5 mol / L. Take 1mL of the DMSO solution of C1 above and place it in a 10mL colorimetric tube, and dilute to volume with HEPES buffer solution to obtain a concentration of 2×10 -6 mol / L, C1 solution with HEPES-DMSO volume ratio of 9:1 as solvent.

[0057] Then, in the solution of the above compound C1, a concentration of 1×10 -2 mol / L Cu(NO 3 ) 2 ·3H 2 O aqueous solution 5 μL (negligible effect on the total volume), to obtain a solution of the complex C1-Cu.

[0058] 2) Prepare Na separately 2 SO 3 ,NaHSO 3 , Na 2 SO 4 , Na 2 S 2 o 3 ·5H 2 O,NaN 3 ,NaCN,KClO 3 ,NaF,NaCl,KBr,KI,NaIO 3 , Na 2 HPO 4 12H 2 O,Na 3 PO4 ,NaOAc·3H 2 O,Na 2 CO 3 ,NaHCO 3 , NaSC...

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Abstract

The invention discloses a complex for rapidly detecting sulfide ions. The complex is a coumarin derivative and copper ion complex, and is applied as a sulfide ion fluorescent sensor. When being used for detecting sulfide ions in an aqueous solution as the fluorescent sensor, the complex shows obvious fluorescence enhancement response, the response speed is fast, and the real-time detection on the sulfide ions can be realized; the detection sensitivity is high, the detection limit is low up to 90nM; the concentration of sulfide ions in a to-be-detected sample can be accurately quantitatively analyzed by utilizing a standard curve; the complex has superhigh selectivity on the sulfide ions, and has almost no interference to other common negative ions; the complex has good biocompatibility and can be successfully applied for fluorescence imaging analysis of the sulfide ions in biocells; and both the fluorescence spectrum and ultraviolet-visible absorption spectrum are responded, and the double-channel detection on the sulfide ions can be realized.

Description

technical field [0001] The invention relates to a complex compound for rapid detection of sulfur ions and its quantitative analysis method and application, belonging to the field of environmental protection. Background technique [0002] With the rapid development of biology, chemical engineering, medicine and environmental disciplines, anions are more and more widely used in these fields, and the recognition and sensing of anions has become a research hotspot. However, the development of anion sensors has been slow compared to the larger number of cation sensors. The design of traditional anion sensors is mainly based on the weak interaction between the host and the guest, so there are disadvantages such as low detection sensitivity and low specificity for the target molecule, especially in the water phase, the strong solvation makes the host - The interaction between the guests becomes weaker, resulting in a decrease in detection sensitivity. These factors greatly limit ...

Claims

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Application Information

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IPC IPC(8): C07D491/16G01N21/64G01N21/78
CPCC07D491/16G01N21/643G01N21/6486G01N21/78
Inventor 程晓红屈少华
Owner 襄阳市大学科技园发展有限公司
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