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A method for preparing citric acid by fermentation

A technology of citric acid and fermentation broth, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of fermentation acidity and sugar-acid conversion rate that need to be further improved

Active Publication Date: 2020-04-14
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermentation acidity and sugar-acid conversion rate still need to be further improved

Method used

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  • A method for preparing citric acid by fermentation
  • A method for preparing citric acid by fermentation
  • A method for preparing citric acid by fermentation

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0054] This preparation example is used to illustrate the preparation of fermentation raw materials.

[0055] (1) 56 kilograms of harvested corn were moistened and stewed in a hot water tank until the water content of the corn was 15% by weight, and then pulverized to obtain a pulverized product with an average particle diameter of 400 microns.

[0056] (2) The pulverized product is slurried at a concentration of 25% by weight. For every gram of the pulverized product, 20 enzyme activity units of amylase (Novozymes, α-amylase, an embodiment of the invention) are added. All of them are amylases), enter the ejector, and enzymatically hydrolyze for 100 minutes under the conditions of 85°C and pH 5.5 to obtain the liquefied liquid of starchy raw materials.

[0057] (3) Press-filter the liquefaction liquid of starchy raw materials with a hydraulic plate and frame filter press to separate the liquefaction liquid of starchy raw materials and the enzymatic hydrolysis filter residue, w...

Embodiment 1-3

[0061] For illustrating the preparation method of citric acid of the present invention.

[0062] Carry out the fermentation of citric acid according to the condition of table 1-table 4. Seed liquid is the Aspergillus niger seed liquid that step (4) obtains. The liquefied liquid and the liquefied clear liquid are respectively the liquefied liquid tank and the liquefied clear liquid tank from step (5) of the preparation example.

[0063] Table 1: The control conditions for level 1-4 tanks are:

[0064]

[0065]

[0066] Table 2: Material flow acceleration for the first incubation stage

[0067]

[0068] Table 3: Material flow acceleration for the second incubation stage

[0069] Example number Primary tank material m 3 / h

liquefied serum m 3 / h

ammonium sulfate m 3 / h

Example 1 30 20 0.45 Example 2 28 22 0.4 Example 3 32 18 0.5

[0070] Table 4

[0071]

[0072] Feed material from No. 5 of the third-stage ta...

Embodiment 4

[0075] This embodiment is used to illustrate the preparation method of citric acid of the present invention.

[0076] The preparation of citric acid was carried out according to the method of Example 1, except that the aeration rate of the third fermentation stage was set to 0.15 vol:vol min. The acidity, conversion rate, single yield and acid supply per hour after fermentation are shown in Table 5.

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PUM

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Abstract

The invention discloses a method for preparing citric acid by fermenting. The method comprises the step of inoculating a citric acid fermenting strain into a citric acid fermentation medium under a fermenting condition, wherein the fermentation procedure comprises the following stages: in the first fermenting stage, feeding the citric acid fermentation medium into a first-level fermenting tank, a fermenting seed solution and a basic nitrogen source, thereby acquiring a first fermenting solution; in the second fermenting stage, feeding the first fermenting solution and an acidic nitrogen source into a second-level fermenting tank, thereby acquiring a second fermenting solution, wherein the throughput is 0.22-0.25 volume: volume. minute which is higher than that in the first, third and fourth fermenting stages; in the third fermenting stage, feeding the second fermenting solution into a third-level fermenting tank, thereby acquiring a third fermenting solution, wherein the tank pressure is 0.07-0.1MPs and is higher than that in the first, second and fourth fermenting stages; in the fourth fermenting stage, feeding the third fermenting solution into a fourth-level fermenting tank, wherein the temperature is at 38-40 DEG C and is higher than that in the first, second and third fermenting stages.

Description

technical field [0001] The invention relates to a method for fermenting and preparing citric acid. Background technique [0002] At present, citric acid manufacturers mainly use batch citric acid fermentation to produce citric acid. This method is extensively controlled and simple, with large production fluctuations, low fermentation conversion rate, and low equipment utilization rate. [0003] The invention patent with the application number 201010255961.9 introduces the process method of segmented control of the batch fermentation process according to the changing trend of RQ value. This process method only controls the temperature at different stages, so as to achieve the purpose of improving the fermentation level. [0004] The invention patent with the application number 201110421212.3 introduces a method of continuous fermentation to produce citric acid. The method is mainly to control the sugar concentration, acidity and nutrients of the medium in different stages of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C12R1/685
CPCC12P7/48
Inventor 卢宗梅杨儒文陈龙廖四祥
Owner COFCO BIOTECHNOLOGY CO LTD