Method for removing heavy metal in enzymatic hydrolysate of sea cucumber by joint use of chelating resins

Abstract

The invention discloses a method for removing heavy metal in enzymatic hydrolysate of sea cucumber by joint use of chelating resins. The method comprises the following steps of connecting chelating resin containing ethylsuleenyl and 2-aminopyridine and PS-EDTA chelating resin in series, and eluting the enzymatic hydrolysate of sea cucumber by the serially connected chelating resin; decomposing the sea cucumber into polypeptide under the action of enzyme, and removing the heavy metal in supernatant by the chelating resins. While the heavy metal ion is removed, the influence to the content and property of other effective ingredients in the enzymatic hydrolysate is avoided; the Hg, Ni, Cu, Cr, Pb and Cd in the enzymatic hydrolysate of sea cucumber can be effectively removed, the other components are used as raw materials of health-care products, and the standard of health-care products is met. The method has the advantages that the method is simple, and the effect is obvious; under the condition of higher selectivity, the influence to the effective ingredients of the product is avoided, multiple types of metal ions can be removed at one time, the adsorbing rate is high, the resin regeneration is easy, the cost is low, and the scaled production requirement is met.

Description

technical field [0001] The invention relates to a method for removing heavy metals in enzymatic hydrolyzate of sea cucumber protein, more particularly, to a method for removing heavy metals in enzymatic hydrolyzate of sea cucumber by combined use of chelating resin. Background technique [0002] In recent years, due to the increasingly serious pollution of heavy metals in soil, rivers and oceans, the content of various heavy metals in products has also become a topic of concern. The components of fruit juice and seafood enzymatic hydrolyzate are complex, and many of them may contain a variety of heavy metals, such as Cu, Zn, Cr, Pb, Cd, As, etc., due to industrial pollution. Heavy metals can interact with proteins in the human body to inactivate them, and can also accumulate in certain organs of the human body. If they exceed the limit that the human body can bear, it will cause poisoning and other diseases, which is extremely harmful to the human body. [0003] Sea cucumbe...

AI Technical Summary

Problems solved by technology

However, it has disadvantages such as high energy consumption and environmental protection.

The adsorption method uses the adsorption properties of the adsorption material to adsorb heavy metals, but because the proteolysis solution is a very complex system, mainly including functional components such as proteins, active peptides and amino acids, the adsorbent is not selective in the adsorption process. Causes loss of nutrients, and is not suitable for large-scale use due to complicated operating procedures

The complexation method utilizes the chelating ability of complexing reagents for heavy metals and is used for the removal of heavy metals. Although it has its advantages in selectivity and removal rate, it is difficult to separate during the removal process. After the complexing agent is added, it only changes The existing form of heavy metals can reduce their toxicity to a certain extent, but there is still no real solution to the problem of excessive heavy metals.

Although the ion exchange resin method has a high extraction rate, is easy to separate, and has little loss of nutrients, its regeneration process is complicated.

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