Positive reference substance applied to in-situ hybridization or immunohistochemical detection and preparation method thereof

Abstract

The invention belongs to the field of biological pathological examination and specifically relates to a positive reference substance applied to in-situ hybridization or immunohistochemical detection and a preparation method thereof. Tissue fragments in the positive reference substance have complete form and sizes suitable for being observed, save antibody reagents and avoid defects that the tissue form is incomplete and test observation is inconvenient caused by smashing due to too large positive tissue paraffin block slices. Furthermore, representative pathological tissue blocks are chosen through HE dyeing, tissue positive expression areas are concentrated, dyeing positive location is accurate, a quality control effect is good, accuracy and scientificity of experiment results are guaranteed, and detection results have full comparability; meanwhile, the preparation method is very simple and convenient, a lot of positive reference substances for multiple detection can be prepared out through once operation, labor and cost are greatly saved, the positive reference substance is suitable for clinical batch work, and detection efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the field of biopathological detection, and in particular relates to a positive reference substance for in situ hybridization or immunohistochemical detection and a preparation method thereof. Background technique [0002] In the current study of clinical histopathological diagnosis and morphology, in situ hybridization and immunohistochemical staining have become indispensable auxiliary techniques for pathological diagnosis, and have been widely used in clinical pathological testing around the world, becoming a routine work in clinical pathological testing. an indispensable part of. These two conventional detection methods not only improve the accuracy of pathological diagnosis, but also penetrate into clinical and basic sciences, and play an inestimable role in exploring the etiology, pathogenesis and scientific research of diseases. For example, EBER in situ hybridization plays a very important role in the pathological diag...

AI Technical Summary

Problems solved by technology

There are mainly two kinds of positive quality control methods commonly used in clinical practice: one is to use the same method as the tissue to be tested to make wax from the tissue known to be positive (abbreviated as positive tissue) every time the tissue to be tested is tested. Then mount the prepared positive control wax block and the wax block of the tissue to be tested on the same glass slide for detection. The positive control of this method is consistent with the experimental conditions of the tissue to be tested, and has the advantages of good comparability of results and small errors. However, this method needs to prepare a positive control wax block every time it detects, which not only needs to consume a large amount of positive control tissues, reagents, etc., but also has cumbersome operations and a large workload, which greatly reduces the detection efficiency; the other is tissue microarray ( TMA) technology, by transferring multiple positive tissues to a new paraffin block at one time, the method of constructing a high-throughput positive tissue array has improved the detection efficiency to a certain extent, but the production process is still cumbersome and the area occupied is relatively small. Large, need to consume a lot of antibody reagents and other shortcomings, restricting its clinical application

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