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Preparation method of hepatitis c virus antibody detection test paper

A hepatitis C virus and antibody detection technology, which can be used in measurement devices, disease diagnosis, instruments, etc. It can improve the sensitivity and specificity, easy operation and rapid diagnosis.

Inactive Publication Date: 2017-08-04
SUZHOU WANMUCHUN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity and specificity of the enzyme-linked immunoassay kit cannot meet the ideal requirements, and the detection cost of the chemiluminescence method is relatively high. The shortcomings of these two methods make it difficult to popularize and apply, and cannot be used as a screening method for hepatitis C virus antibodies. important means

Method used

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0024] (1) Containers and materials

[0025] Container equipment: beakers, measuring cylinders, volumetric flasks, glass rods, magnetic stirrers, electronic balances, pipette guns, film spraying equipment, cutting machines, strip cutting machines, sealing machines, shell pressing machines, pH meters, blast drying box.

[0026] Reagent accessories: chloroauric acid, trisodium citrate, K 2 CO 3 , sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, absolute ethanol, Tris (trishydroxymethylamino...

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Abstract

The invention discloses a preparation method of a HCV (hepatitis c virus) antibody detection test paper. The preparation method comprises the following steps of using a mixed liquid labeled by HCV labeled monoclonal antibody and HCV labeled antigen to spray gold to a fiber cellulose membrane by membrane dotting and gold spraying equipment, so as to obtain a gold pad; using a HCV-coated antigen detection line coating liquid and a contrast line coating liquid to respectively score on a nitrate cellulose membrane, treating, and adhering with a polyvinyl chloride substrate, so as to obtain the membrane-dotted polyvinyl chloride substrate; assembling a sample pad, the gold pad, the membrane-dotted polyvinyl chloride substrate and water absorbing paper, and cutting into the detection test paper. By adopting the type, the prepared detection test paper has the advantages that on the basis of immune lateral flow chromatography technique, the containing of HCV antibody in a blood sample can be judged by manual operation and naked eye reading, the diagnosis speed is quick, the result is accurate, and the injury to a body of the detected person is avoided.

Description

technical field [0001] The invention belongs to the technical field of biological applications, in particular to a preparation method of a hepatitis C virus antibody detection test paper. Background technique [0002] Hepatitis C virus, namely HCV, referred to as hepatitis C, hepatitis C, is a kind of viral hepatitis caused by hepatitis C virus infection. Hepatitis C is a globally prevalent infectious disease. The average global prevalence of hepatitis C is 3.0%. There are 3 to 4 million new HCV infections every year, and an estimated 170 million chronic HCV infections. The most important modes of transmission of HCV include transmission through blood transfusion and blood products, exposure through damaged skin and mucous membranes, sexual intercourse with infected persons, and high-risk sexual behaviors. In the past, the high rate of HCV infection among blood donors was the main reason for the prevalence of HCV. With the active screening of blood donors in most countries...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56983G01N33/558G01N2800/00
Inventor 丁锦勤黄政马军
Owner SUZHOU WANMUCHUN BIOLOGICAL TECH
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