Preparation method of hepatitis c virus antibody detection test paper

A hepatitis C virus and antibody detection technology, which can be used in measurement devices, disease diagnosis, instruments, etc. It can improve the sensitivity and specificity, easy operation and rapid diagnosis.

Inactive Publication Date: 2017-08-04
SUZHOU WANMUCHUN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The sensitivity and specificity of the enzyme-linked immunoassay kit cannot meet the ideal requirements, and the detection cost of the chemiluminescence method is relatively high. The shortcomings of these two methods make it difficult to popularize and apply, and cannot be used as a screening method for hepatitis C virus antibodies. important means

Method used

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0024] (1) Containers and materials

[0025] Container equipment: beakers, measuring cylinders, volumetric flasks, glass rods, magnetic stirrers, electronic balances, pipette guns, film spraying equipment, cutting machines, strip cutting machines, sealing machines, shell pressing machines, pH meters, blast drying box.

[0026] Reagent accessories: chloroauric acid, trisodium citrate, K 2 CO 3 , sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, absolute ethanol, Tris (trishydroxymethylamino...

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Abstract

The invention discloses a preparation method of a hepatitis C virus antibody detection test paper, which comprises the steps of: spraying gold on a glass cellulose film with a mixed solution of HCV-labeled monoclonal antibody and HCV-labeled antigen label through a film-spraying gold equipment to obtain a gold pad , use the HCV coating antigen detection line coating solution and the control line coating solution to draw lines on the nitrocellulose membrane respectively, and paste it on the polyvinyl chloride bottom plate after processing to obtain the polyvinyl chloride bottom plate of the spot film; the sample pad , gold pad, polyvinyl chloride bottom plate with dot film and absorbent paper are assembled together, and cut into test paper. Through the above-mentioned method, the detection test paper obtained in the present invention is based on the immunological lateral flow chromatography technology, and can judge whether the blood sample contains hepatitis C virus antibody through manual operation and naked eye reading. bodily harm.

Description

technical field [0001] The invention belongs to the technical field of biological applications, in particular to a preparation method of a hepatitis C virus antibody detection test paper. Background technique [0002] Hepatitis C virus, namely HCV, referred to as hepatitis C, hepatitis C, is a kind of viral hepatitis caused by hepatitis C virus infection. Hepatitis C is a globally prevalent infectious disease. The average global prevalence of hepatitis C is 3.0%. There are 3 to 4 million new HCV infections every year, and an estimated 170 million chronic HCV infections. The most important modes of transmission of HCV include transmission through blood transfusion and blood products, exposure through damaged skin and mucous membranes, sexual intercourse with infected persons, and high-risk sexual behaviors. In the past, the high rate of HCV infection among blood donors was the main reason for the prevalence of HCV. With the active screening of blood donors in most countries...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56983G01N33/558G01N2800/00
Inventor 丁锦勤黄政马军
Owner SUZHOU WANMUCHUN BIOLOGICAL TECH
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