Method for preparing 6-O-glucose-cycloastragenol by transforming astragaloside through aspergillus carbonarius

A technology of astragaloside IV and cycloastragaloside, which is applied in the field of medicine and chemical industry, can solve the problems of low conversion efficiency, pollute the environment, many by-products, etc., and achieve the effects of high conversion rate, reduced environmental damage, and reduced energy consumption.

Active Publication Date: 2017-08-11
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to select and adopt a method for microbial conversion to convert astragaloside IV to obtain 6-O-glucose-cycloastragenol, to overcome the many by-products and low conversion rate in the existing 6-O-glucose-cycloastragenol preparation technology. Low efficiency, high cost, environmental pollution and other disadvantages

Method used

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  • Method for preparing 6-O-glucose-cycloastragenol by transforming astragaloside through aspergillus carbonarius
  • Method for preparing 6-O-glucose-cycloastragenol by transforming astragaloside through aspergillus carbonarius

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Effect test

Embodiment 1

[0027] Take the Aspergillus charcoal lyophilized powder and place it on the inclined surface of the test tube containing PDA medium for activation and culture, and then transfer it to the PDA flat medium for cultivation. After the Aspergillus charcoal completely grows spores, add sterile water to the flat medium Spore concentration is 6×10 8 A / mL Aspergillus charcoal spore suspension is the seed liquid for the fermentation of the strain (all operations are performed aseptically in an ultra-clean bench).

[0028] Fermentation liquid culture medium formula: substrate is 10% by mass astragaloside IV, concentration is 1mg / mL, astragalus powder concentration is 2g / L, yeast powder concentration is 10g / L, ammonium sulfate concentration is 2g / L, KH 2 PO 4 The concentration is 4g / L, MgSO 4 ·7H 2 The O concentration is 1g / L, the volume percentage of Tween 80 is 0.1%, and the initial pH is 4.5. The liquid fermentation medium was prepared according to the formula of the medium.

[0029] Take 1...

Embodiment 2

[0034] Take the Aspergillus charcoal lyophilized powder and place it on the inclined surface of the test tube containing PDA medium for activation and culture, and then transfer it to the PDA flat medium for cultivation. After the Aspergillus charcoal completely grows spores, add sterile water to the flat medium Spore concentration is 6×10 8 A / mL Aspergillus charcoal spore suspension is the seed liquid for the fermentation of the strain (all operations are performed aseptically in an ultra-clean bench).

[0035] Fermentation liquid culture medium formula: substrate is 10% by mass astragaloside IV, concentration is 1mg / mL, astragalus powder concentration is 2g / L, yeast powder concentration is 10g / L, ammonium sulfate concentration is 2g / L, KH 2 PO 4 The concentration is 4g / L, MgSO 4 ·7H 2 The O concentration is 1g / L, the volume percentage of Tween 80 is 0.1%, and the initial pH is 4.5. The liquid fermentation medium was prepared according to the formula of the medium.

[0036] Take 1...

Embodiment 3

[0041] Take the Aspergillus charcoal lyophilized powder and place it on the inclined surface of the test tube containing PDA medium for activation and culture, and then transfer it to the PDA flat medium for cultivation. After the Aspergillus charcoal completely grows spores, add sterile water Spore concentration is 6×10 8 A / mL Aspergillus charcoal spore suspension is the seed liquid for the fermentation of the strain (all operations are performed aseptically in an ultra-clean bench).

[0042] Fermentation liquid culture medium formula: substrate is 10% by mass astragaloside IV, concentration is 1mg / mL, astragalus powder concentration is 2g / L, yeast powder concentration is 10g / L, ammonium sulfate concentration is 2g / L, KH 2 PO 4 The concentration is 4g / L, MgSO 4 ·7H 2 The O concentration is 1g / L, the volume percentage of Tween 80 is 0.1%, and the initial pH is 4.5. The liquid fermentation medium was prepared according to the formula of the medium.

[0043] Take 10mL of spore concen...

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Abstract

The invention discloses a method for preparing 6-O-glucose-cycloastragenol by transforming astragaloside through aspergillus carbonarius, and particularly relates to a method for preparing the 6-O-glucose-cycloastragenol in a transformed manner by using the astragaloside as a substrate to ferment through aspergillus carbonarius. CICC 41254 on the premise of using a proper amount of astragalus root powder as an inducer. Fermentation liquor obtained by fermentation is extracted through water saturated n-butyl alcohol, is subjected to column chromatography on silica gel and is recrystallized with ethanol, and finally, the 6-O-glucose-cycloastragenol product with the purity being 95% or above is obtained finally. The 6-O-glucose-cycloastragenol is prepared by a microbe transformation method. The method has the characteristics that the astragaloside as a substrate is almost transformed totally, the transformation rate is high, the method is simple and practicable, the cost is low, and the method is pollution-free to environment, and is suitable for industrialized production.

Description

Technical field [0001] The invention belongs to the field of medicine and chemical industry, and specifically relates to a method for preparing 6-O-glucose-cycloastragalol by using Aspergillus charcoal to transform astragaloside IV. Background technique [0002] Astragalus is a traditional Chinese medicine. Its medicinal use has a history of more than 2,000 years. It has the functions of enhancing the body’s immune function, protecting the liver, diuresis, anti-aging, anti-stress, lowering blood pressure, and a wide range of antibacterial effects; cycloastragalol saponins are The main active ingredient in astragalus Chinese medicine belongs to the cycloaltinane tetracyclic triterpene saponins. It has the functions of strengthening immunity, increasing energy, resisting fatigue, protecting liver, scavenging free radicals and inhibiting osteoclasts. Use value. [0003] 6-O-glucose-cycloastragenol (CMG) belongs to the saponins of cycloastragalol, and its structure was first reported ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/66
CPCC12P33/20
Inventor 袁其朋程磊雨
Owner BEIJING UNIV OF CHEM TECH
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