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Method for preparing sensor for detecting kaempferide molecular imprinting

A molecular imprinting and kaempferol technology, applied in the direction of electrochemical variables of materials, can solve the problems of poor regeneration and reversibility, high detection limit, slow electron transfer speed and response, and achieve high affinity and selectivity. Effect

Inactive Publication Date: 2017-08-11
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of nano-gold graphene modification solution: In the reactor, add deionized water: 92mL, chloroauric acid: 2g, graphene oxide: 6g, ultrasonic at room temperature for 15min, and disperse evenly to obtain nano-gold graphene Correction fluid;

[0024] (2) Preparation of nano-gold graphene-modified glassy carbon electrode: Surface polishing of glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonic cleaning with twice distilled water, ethanol washing, drying, and drying on the surface of glassy carbon electrode Add 19 μL of nano-gold graphene modification solution dropwise, place it under an infrared lamp, and evaporate the solvent to obtain a nano-gold graphene-modified glassy carbon electrode;

[0025] (3) Preparation of kaempferol molecularly imprinted polymer: In the reactor, add ethanol: 60 mL, ethylene glycol dimethacrylate: 12 g, styrene: 6 g, 1-vinyl-3-butyl Hexafluorophosphate: 32g, azobisisobutyronitrile: 1g, kaemp...

Embodiment 2

[0028] (1) Preparation of nano-gold graphene modification solution: In the reactor, add deionized water: 9.5mL, chloroauric acid: 0.1g, graphene oxide: 0.4g, ultrasonically 15min at room temperature, and disperse evenly to obtain nano Gold graphene modification fluid;

[0029] (2) Preparation of nano-gold graphene-modified glassy carbon electrode: Surface polishing of glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonic cleaning with twice distilled water, ethanol washing, drying, and drying on the surface of glassy carbon electrode Add 18 μL of nano-gold graphene modification solution dropwise, place it under an infrared lamp, and evaporate the solvent to obtain a nano-gold graphene-modified glassy carbon electrode;

[0030] (3) Preparation of kaempferol molecularly imprinted polymer: In the reactor, add ethanol: 6.3 mL, ethylene glycol dimethacrylate: 1.0 g, styrene: 0.5 g, 1-vinyl-3- Butyl hexafluorophosphate: 3.0g, azobisisobutyronitri...

Embodiment 3

[0033] (1) Preparation of nano-gold graphene modification solution: In the reactor, add deionized water: 9mL, chloroauric acid: 0.3g, graphene oxide: 0.7g, ultrasonic at room temperature for 15min, and disperse evenly to obtain nano-gold Graphene modification fluid;

[0034] (2) Preparation of nano-gold graphene-modified glassy carbon electrode: Surface polishing of glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonic cleaning with twice distilled water, ethanol washing, drying, and drying on the surface of glassy carbon electrode Add 20 μL of nano-gold graphene modification solution dropwise, place it under an infrared lamp, and evaporate the solvent to obtain a nano-gold graphene-modified glassy carbon electrode;

[0035] (3) Preparation of kaempferol molecularly imprinted polymer: In the reactor, add ethanol: 5.6 mL, ethylene glycol dimethacrylate: 1.3 g, styrene: 0.7 g, 1-vinyl-3- Butyl hexafluorophosphate: 3.1g, azobisisobutyronitrile: ...

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PUM

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Abstract

The invention discloses a method for preparing a sensor for detecting kaempferide molecular imprinting. The method is characterized in that first chloroauric acid and graphene oxide to modify a glassy carbon electrode, and obtaining a nanogold graphene-modified glassy carbon electrode; adding 44-50% of ethanol, 10-14% of ethylene glycol dimethacrylate, 5-8% of styrene, 30-35% of 1-vinyl-3-butyl hexafluorophosphate, 1.0-2.0% of azodiisobutyronitrile and 2.0-5.0% of kaemperfol into a reactor, performing mixing and dissolution, perform stirring reaction for 28-30 h at 55+ / -2 DEG C, adopting methanol : acetic acid mixed solution to remove template molecules to obtain kaempferide molecularly imprinted polymer; dispensing the molecularly imprinted polymer to the nanogold graphene-modified glassy carbon electrode, and obtaining the kaempferide molecular imprinting sensor. The sensor is high in compatibility and selectivity. The sensor is high in sensitivity and good in specificity, has quickness in detection and can be reused.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a method for preparing a molecularly imprinted sensor for detection of kaempferol, specifically based on the specific recognition of molecular imprinting, for the detection of drugs , food, and kaempferol in biological samples. Background technique [0002] Kaempferol (scientific name is 3,5,7-trihydroxy-4'-methoxyflavone) whose molecular formula is C 16 h 12 o 6 , with a relative molecular mass of 300.26, is a bioflavonoid with a special molecular structure and has a variety of pharmacological activities. Kaempferol is yellow crystalline powder, slightly soluble in water, soluble in hot ethanol, ether and alkali. Kaempferol can activate the activity of melanocyte tyrosinase, promote the proliferation of melanocytes, and increase the synthesis of melanin. , anti-ulcer, choleretic diuretic,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/30
Inventor 李慧芝翟玉博许崇娟
Owner UNIV OF JINAN
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