Application of MBL in preparing drug for preventing or treating disease induced by effector T cell

A technology for treating effects and diseases, applied in metabolic diseases, skin diseases, bone diseases, etc., to achieve the effect of strong regeneration ability and rich sources

A technology for treating effects and diseases, applied in metabolic diseases, skin diseases, bone diseases, etc., to achieve the effect of strong regeneration ability and rich sources

CN107050436AActive Publication Date: 2017-08-18XINXIANG MEDICAL UNIV
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  • Application of MBL in preparing drug for preventing or treating disease induced by effector T cell
  • Application of MBL in preparing drug for preventing or treating disease induced by effector T cell
  • Application of MBL in preparing drug for preventing or treating disease induced by effector T cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Experimental Materials

[0032] 1. Source of cells used in the experiment

[0033] Newborn umbilical cord blood was collected from the third affiliated hospital of Xinxiang Medical College and the full-term healthy newborns of the Department of Obstetrics and Gynecology of Xinxiang First People's Hospital. The blood was collected from the placenta umbilical vein immediately after delivery and put into a blood collection bag containing 28 mL of preservation solution. All blood samples were delivered to the laboratory within 4 hours after sampling.

[0034] 2. Main reagents

[0035] The medium RPMI1640, Fetal bovine serum (FBS), and 10×PBS solution were all purchased from ThermoFisher Scientific.

[0036] Lymphocyte separation solution (Ficoll-Paque PLUS) is a sterile endotoxin test solution (<0.12EU / mL) of FicollTM PM400 and sodium diatrizoate with a density of 1.077 g / mL, purchased from GE Healthcare Life Sciences.

[0037] Anti-human CD3 monoclonal antibody (CD3mAb), anti-human ...

Embodiment 2

[0094] Experimental Materials

[0095] 1. Source of cells used in the experiment

[0096] Reference example 1

[0097] 2. Main reagents

[0098] Reference example 1

[0099] 3. Main instruments and equipment

[0100] Reference example 1

[0101] 4. Preparation of common reagents

[0102] Reference example 1

[0103] experimental method

[0104] 1. Preparation of cord blood mononuclear cells

[0105] Reference example 1

[0106] 2. Coated with anti-CD3mAb

[0107] Reference example 1

[0108] 3. Magnetic sorting of CD4+CD25-T cells and CD4+CD25+T cells

[0109] Sample preparation

[0110] Density gradient centrifugation was used to separate peripheral mononuclear cells. In order to remove platelets, the cells were resuspended in a buffer and centrifuged at 200×g for 10-15 minutes. The supernatant was carefully aspirated and washed repeatedly.

[0111] Magnetic labeling of non-CD4+ T cells

[0112] The entire process should be completed quickly and the cells should be kept in a pre-cooled state to pre...

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Abstract

The invention discloses an application of MBL in preparing a drug for preventing or treating disease induced by an effector T cell and belongs to the technical field of a novel application of mannan binding lectin. The key point of the technical scheme is as follows: the invention relates to the application of MBL in preparing a drug for preventing or treating infectious diseases, autoimmune diseases or / and allergic diseases induced by the effector T cell, wherein MBL realizes functions of the drug by enhancing iTreg cell and inhibiting proliferation of the effector T cell. Through researches, a novel factor of adjusting the inhibition function of the Tregs cell is obtained, that is, MBL directly enhances the inhibition action of the Tregs cell on proliferation of the effector T cell. The application disclosed by the invention highlights a novel function of MBL, and MBL not only plays an important anti-infection role in inherent immunity, but also can be a pertinent target spot for treating various autoimmune diseases and inflammatory diseases in intervention of the function of the Tregs cell.

Description

Technical field [0001] The invention relates to the technical field of new uses of mannan-binding lectins, in particular to the application of MBL in the preparation of drugs for preventing or treating effector T cell-induced diseases. Background technique [0002] Mannan-binding lectin (mannan-binding lectin, MBL) is a highly conserved plasma protein mainly secreted by hepatocytes, and is a member of the collectins family in the C-type lectin superfamily. MBL is also an acute phase reactive protein, and its plasma concentration can increase by 2-3 times during stress (such as pathogen infection, surgery, etc.). MBL, known as the ante-antibody, uses its carbohydrate recongnition domain (CRD) to broadly recognize sugar structures on the surface of a variety of pathogens such as bacteria, viruses, parasites, and fungi, including D-mannose. Sugar, L-fucose, N-acetylglucosamine, N-acetylmannosamine, etc. Its collagen-like region (CLR) binds to MBL-associated serine protease (MBL-MA...

Claims

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Application Information

Patent Timeline
18 Aug 2017
Publication
CN107050436A
IPC
A61K38/36; A61P33/02; A61P33/06; A61P31/04; A61P31/22; A61P31/18; A61P1/16; A61P31/20; A61P31/14; A61P37/02; A61P3/10; A61P29/00; A61P19/02; A61P17/00; A61P5/16; A61P17/14; A61P37/08; A61P11/02; A61P11/06
CPC
A61K38/36; Y02A50/30
Inventors
王凡平; 王明永