Preparation method of pure natural concentrated type scindapsus aureus nutrient solution
A concentrated and nutrient solution technology, applied in liquid fertilizers, organic fertilizers, fertilizer mixtures, etc., can solve the problems of waste of resources, high cost of raw materials for seaweed nutrient solution production, environmental pollution, etc.
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Embodiment 1
[0023] Wash the kelp leftovers with tap water; then soak them in tap water for 24 hours, during which the water is changed every 3 hours; the soaked leftovers are chopped with a knife, and the broken kelp is pulverized by a colloid mill. After crushing, the kelp powder and distilled water are mixed at a volume ratio of 1:5, and the suspension is processed by a low-temperature ultra-high pressure continuous flow cell crusher; the working conditions of the crusher: pressure 50 MPa, continuous flow 2.1 liters / hour, crushing temperature 5°C . Add cellulase and β-glucosidase to kelp homogenate at an enzyme concentration of 500 mg / L, add cellulase and β-glucosidase at a mass ratio of 1:1, adjust the pH to 4.5, and stir at a speed of 100 rpm. After stirring for 5 minutes, the stirring speed was adjusted to 50 rpm, and the entire enzyme biocatalytic conversion reaction process was 3 hours, and the temperature was controlled at 45°C. After the enzymatic hydrolyzate was treated at 80°C...
Embodiment 2
[0025] Wash the kelp leftovers with tap water; then soak them in tap water for 24 hours, during which the water is changed every 3 hours; the soaked leftovers are chopped with a knife, and the broken kelp is pulverized by a colloid mill. After pulverization, the kelp mince is mixed with distilled water at a volume ratio of 1:20, and the suspension is processed by a low-temperature ultra-high pressure continuous flow cell crusher; working conditions of the crusher: pressure 150 MPa, continuous flow 2.1 liters / hour, crushing temperature 5°C . Add cellulase and β-glucosidase to kelp homogenate at an enzyme concentration of 500 mg / L, add cellulase and β-glucosidase at a mass ratio of 5:1, adjust the pH to 6.5, and stir at a speed of 200 rpm. After stirring for 15 minutes, the stirring speed was adjusted to 150 rpm, and the entire enzyme biocatalytic conversion reaction process was 3 hours, and the temperature was controlled at 65°C. After the enzymatic hydrolyzate was treated at ...
Embodiment 3
[0027] Wash the kelp leftovers with tap water; then soak them in tap water for 24 hours, during which the water is changed every 3 hours; the soaked leftovers are chopped with a knife, and the broken kelp is pulverized by a colloid mill. After crushing, the kelp powder and distilled water are mixed at a volume ratio of 1:10, and the suspension is processed by a low-temperature ultra-high pressure continuous flow cell crusher; the working conditions of the crusher: pressure 100 MPa, continuous flow 2.1 liters / hour, crushing temperature 5 °C . Add cellulase and β-glucosidase to the kelp homogenate at an enzyme concentration of 10,000 mg / liter, add cellulase and β-glucosidase at a mass ratio of 3:1, adjust the pH to 5.5, and stir at a speed of 150 rpm. After stirring for 10 minutes, the stirring speed was adjusted to 100 rpm, and the entire enzyme biocatalytic conversion reaction process was 3 hours, and the temperature was controlled at 55°C. After the enzymolyzed product was t...
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