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Preparation and Application of Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen

An immune sensor and carcinoembryonic antigen technology, applied in the field of nanomaterials, can solve the problems of susceptibility to high temperature, acid and alkali conditions, limited wide application, complicated acquisition process, etc., to achieve sensitive signal amplification, reduce complexity and Cost, the effect of enhancing the photocurrent signal

Inactive Publication Date: 2018-10-12
UNIV OF JINAN
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AI Technical Summary

Problems solved by technology

[0003] The commonly used recognition elements in traditional immunosensors are antibodies, which are usually produced by cell lines or animals. Defects greatly limit its wide application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]Embodiment 1: photoelectrochemical immunosensor is used for the detection of carcinoembryonic antigen

[0024] (1) To prepare ZnO NRs-NSs / FTO electrodes, first prepare ZnO NRs / FTO electrodes by electrodeposition method: after ultrasonically cleaning FTO with ethanol, acetone, and secondary water for 10 min, spin-coat the FTO surface at a concentration of 40 mM Zinc acetate seed solution with a rotating speed of 1000 r / min and a time of 30 s, and then the spin-coated FTO was dried at 120 °C for 10 min, and the above-mentioned "spin coating-drying" process was repeated for 6 times, and the FTO electrode A three-electrode system composed of Ag / AgCl reference electrode and platinum counter electrode was used for electrodeposition operation, the deposition potential was 0.0V, and the deposition electrolyte was zinc nitrate with a concentration of 50 mM, ammonium acetate with a concentration of 50 mM and a concentration of 70 ethylenediamine mixture, the deposition temperature...

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Abstract

The invention discloses a preparation and application study of photoelectrochemical immunosensor for detection of carcinoembryonic antigen. ZnO nanorod-nanosheet with three-dimensional layered structure is grown on transparent conductive tin dioxide glass doped with fluoride, the ZnO nanorod-nanosheet is used to load a large amount of CdS quantum dots doped with manganese, aptamers for identifying carcinoembryonic antigen and DNA probes marked with CdTe / CdSe core shell quantum dots and hybridized with the aptamers to form a multivariate sensitizing structure based on ZnO, so that an initial signal amplification is achieved; in combination with the aptamers for specific recognition of carcinoembryonic antigen, the DNA probes are disintegrated from the hybridization to depart from the weakened sensitizing effect on the electrode surface and the steric hindrance of the conjugate formed by the carcinoembryonic antigen and the aptamers so that a further signal amplification is achieved, and further the sensitive detection of carcinoembryonic antigen is realized.

Description

technical field [0001] The invention relates to the fields of nanometer material technology, photoelectrochemical signal detection technology and immunoassay detection technology, and more specifically relates to the preparation and application of a photoelectrochemical immunosensor for sensitive detection of carcinoembryonic antigen. Background technique [0002] As a very important tumor marker, carcinoembryonic antigen plays an important role in the clinical diagnosis and treatment of cancer. In recent years, various analytical methods have been developed for the detection of carcinoembryonic antigen, such as fluorescence, electrochemistry, electrochemiluminescence, colorimetry and other analytical methods. Although these methods can realize the detection of carcinoembryonic antigen, they have disadvantages such as complex operation, low sensitivity, time-consuming, and high cost. As a new analytical method, photoelectrochemical analysis has attracted widespread attentio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N27/327G01N27/416B82Y30/00B82Y40/00B82Y5/00
CPCB82Y5/00B82Y30/00B82Y40/00G01N27/3278G01N27/416G01N33/57473G01N33/57484
Inventor 杨红梅张彦李丽徐金梦于京华葛慎光颜梅
Owner UNIV OF JINAN
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