Bacillus cereus and application thereof in degradation of feathers

A technology for Bacillus cereus and feathers, which is applied in the application field of environmental resources in aquaculture industry, can solve problems such as undisclosed simultaneous degradation of feathers and the like, and achieve the effects of good degradation and ecological promotion.

Active Publication Date: 2017-08-29
JINLING INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] CN106350558A discloses the use of Bacillus cereus and keratinase to jointly degrade chicken feathers, but it does not disclose that it can simultaneously degrade the feathers of chickens, ducks, geese, and pigeons, and the combined degradation effect is better than that of Bacillus cereus or keratinase alone.
At present, there is no report on the simultaneous degradation of chicken, duck, goose and pigeon feathers by Bacillus cereus at home and abroad

Method used

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  • Bacillus cereus and application thereof in degradation of feathers
  • Bacillus cereus and application thereof in degradation of feathers
  • Bacillus cereus and application thereof in degradation of feathers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Culture identification of strains.

[0049] 1. Obtaining the original strain

[0050] A few healthy pigeons were taken from a pigeon farm in Nanjing, and the pigeons were dissected next to an alcohol lamp, and the contents of their cecum were directly streaked and inoculated on a common agar plate with a sterilized inoculation loop. For the morphology of a single colony, take the diameter of about 6mm, the irregular edge is milky white and opaque, and the surface is rough and white wax-like colony for further isolation and culture, and pure culture is carried out after obtaining a single colony. Bacterial isolation and culture results: Through bacterial isolation and culture, a pure cultured bacterium was obtained, named NJ-02. The isolated bacteria were gram stained and spore stained, and then observed under the oil lens of a light microscope. The results were G+ medium-sized bacilli with spores, most of them were single, a few were 3-5 short chains, the s...

Embodiment 2

[0071] Example 2: Observation of the degradation morphology of the feathers of chickens, ducks, geese and pigeons by bacteria.

[0072] In order to observe whether the isolated and cultured NJ-02 Bacillus cereus can degrade the feathers of chickens, ducks, geese and pigeons, NJ-02 Bacillus cereus was designed to degrade the feathers of chickens, ducks, geese and pigeons. Observation test on the degradation morphology of poultry feathers. Add 5 ml of degraded feather basal medium (K 2 HPO 4 0.4g / L, NaCl 0.5g / L, FeSO 4 0.1g / L initial pH 7.5), and a piece of clean poultry feathers (the feathers of chickens, ducks, geese and pigeons are all treated and observed in the same way), autoclave 15 pounds at 121°C for 15min, after cooling, inoculate an appropriate amount of bacteria isolated and cultured, set at 37 The results were observed after culturing in a ℃ incubator for 5d-15d.

[0073] 1. Degradation of goose feathers: Feather flakes on the 5th day: the feather trunk is no ...

Embodiment 3

[0089] Example 3: Determination of Feather Degradation Residues of Bacteria on Chicken, Duck, Goose, Pigeon and Other Poultry

[0090] Feather meal pretreatment: soak the collected feathers in 0.04% NaOH solution at 20-25°C for 1 hour, rinse with clean water, filter, dry, and pulverize with a particle size between 750 μm and 1200 μm for later use.

[0091] Take 24 conical flasks with a size of 250ml, add 1 gram of chicken feather powder with a particle size of 750μm to 1200μm and 50ml of fermentation medium (fermentation medium formula is as follows: K 2 HPO 4 0.4g / L, FeSO 4 0.1g / L, NaCl 0.5g / L, solvent is water, initial pH 7.5, autoclave), autoclave 15 pounds at 121°C for 15min, and wait for cold use. Then divided into two groups, the first group of 12, each inoculated with an appropriate amount of isolated and cultured bacteria NJ-02 (not less than 10 9 CFU / ml), another group of 12 were used as blank control, placed in a shaking incubator at 37°C at 150 rpm / min on the 3...

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Abstract

The invention discloses Bacillus cereus capable of degrading feathers. The Bacillus cereus is preserved in China Center for Type Culture Collection on March 30th, 2017 with the preservation number of CCTCC NO:M2017155. The invention also discloses application of the bacillus cereus. The new strain disclosed by the invention can be directly applied to degradation of multiple feathers of chickens, ducks, gooses and pigeons, especially the capability of degrading feathers of gooses is the strongest, so that recycling of multiple feathers of chickens, ducks, gooses and pigeons is facilitated, and ecological and recirculating culture is promoted. The new strain belongs to a safe microorganism and can be used in the fields of degradation of multiple feathers of chickens, ducks, gooses and pigeons, poultry farming waste fermentation and biofertilizer.

Description

technical field [0001] The invention relates to the application field of environmental resources in aquaculture, in particular to a Bacillus cereus capable of effectively degrading feathers and its application. Background technique [0002] With the intensive development of the poultry industry, breeding enterprises, especially large and medium-sized livestock farms, produce a large amount of waste such as feces, feathers, bone meal, blood residues, etc. every day while providing meat, poultry and eggs. If not handled properly, it will often pollute the environment and spread pathogens. Feathers, in particular, are more difficult to recycle than other wastes due to their high content of keratin, which is difficult to degrade. According to research reports, the content of keratin in feathers is as high as 80%, and it is rich in lysine, tryptophan, threonine, methionine, histidine, proline, glycine and some other vitamins, macroelements and trace amounts Due to the lack of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/26A23K10/12C05F15/00C12R1/085
CPCC05F1/005C12N1/205C12R2001/085C05F11/08
Inventor 戴鼎震沙奕羽陆林豪方光远谭成成金李萍夏庭轶
Owner JINLING INST OF TECH
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