Method for detecting single biological marker by using total internal reflection fluorescent microscope

A biomarker and fluorescence microscope technology, applied in the field of biological detection, can solve the problems of unsure of the treatment method, missing the best treatment opportunity, and exacerbation of the disease in sick animals, and achieves convenient and easy detection steps, short detection time, and low background low noise effect
CN107101981AInactive Publication Date: 2017-08-29NANJING AGRICULTURAL UNIVERSITY
4 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2017-08-29
Estimated Expiration
Not applicable Β· inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for detecting a biological marker by using a total internal reflection fluorescent microscope. The method comprises the following steps of utilizing biotin-avidin connection, and antigen and antibody reaction or nucleic acid molecule and probe reaction, using a total internal reflection fluorescent exciting type to excite a detection molecule of a fluorescent marker which is fixed in the 100nm range of the carrier surface, and collecting a fluorescent molecule signal by the fluorescent microscope, so as to obtain the number of to-be-detected biology markers. The method has the advantages that because the free fluorescent molecules in the solution are not excited, the background noise of the collected fluorescent signal is lower, and the signal to noise ratio is very high; the fluorescent signal of the single molecule can be distinguished, so that the detection sensitivity is high; the detection method can be used for detecting various types of biological markers, and an important meaning is realized for the finding of pathogen in diseases and the preventing and controlling of diseases.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of biological detection, in particular to a method for detecting a single biological marker using an inverted microscope based on total internal reflection fluorescence. Background technique

[0002] Many diseases caused by pathogenic microorganisms occur and spread widely in the world today, seriously endangering the health of humans, animals and plants, and affecting the quality of human life. Understanding the properties of pathogenic microorganisms is essential for disease prevention and treatment. There are many methods for detecting pathogens, such as enzyme-linked immunosorbent assay (ELISA), Western blot (Western Blot) and polymerase chain reaction (PCR), etc., but these methods have more or less problems, such as operation Complexity, high cost, low sensitivity and other disadvantages. It is difficult to detect trace pathogens in biological tissues or cells using conventional methods, which makes ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More