Preparation method of nano barium carbonate by using red blood cells as bioreactor

A nano-barium carbonate and bioreactor technology, applied in the direction of barium carbonate, calcium carbonate/strontium/barium, nanotechnology, etc., to achieve good biocompatibility, uniform particle size, and good dispersibility

Inactive Publication Date: 2017-09-01
HENAN NORMAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Most of the relevant reports use microorganisms as reactors, such as: yeast, Escherichia coli, Shewanella, e

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  • Preparation method of nano barium carbonate by using red blood cells as bioreactor
  • Preparation method of nano barium carbonate by using red blood cells as bioreactor

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Embodiment 1

[0021] Remove the upper layer of plasma by centrifugation from whole pig blood and wash with normal saline for 3 times to obtain red blood cell pellet, suspend 0.5mL red blood cell pellet in 5mL containing 40mM BaCl 2 Add 0.5g of glucose to normal saline, react in an ice-water bath for 3 hours, then centrifuge and wash to fully wash away the BaCl outside the cells 2 , collected the centrifuged cell pellet and resuspended in 5 mL containing 40 mM Na 2 CO 3 placed in a shaker (oscillating rate of 90rpm) at 10°C for 3 hours, then centrifuged and washed to obtain functionalized erythrocytes containing barium carbonate. The functionalized erythrocytes were pulverized, separated and purified to obtain erythrocytes with an average particle size of 4.2nm. Barium Carbonate Nanoparticles.

[0022] figure 1 It is the XRD spectrogram of the barium carbonate nanoparticle that the present embodiment makes, and it is consistent with the standard collection of illustrative plates (JCPDSNO....

Embodiment 2

[0024] Centrifuge the whole sheep blood to remove the upper layer of plasma and wash with normal saline for 3 times to get red blood cell pellet, suspend 0.5mL red blood cell pellet in 5mL containing 30mM BaCl 2 Add 0.5g of glucose to normal saline, react in an ice-water bath for 2 hours, then centrifuge and wash to fully wash away the BaCl outside the cells 2 , collected the centrifuged cell pellet and resuspended in 5 mL containing 30 mM Na 2 CO 3 placed in a shaker (oscillation rate of 50rpm) at 4°C for 5 hours, then centrifuged and washed to obtain functionalized erythrocytes containing barium carbonate. The functionalized erythrocytes were pulverized, separated and purified to obtain an average particle size of about 4.2nm barium carbonate nanoparticles.

Embodiment 3

[0026] Centrifuge the upper layer of plasma from the whole rabbit blood and wash it with normal saline for 3 times to obtain the red blood cell pellet. Suspend 0.5mL red blood cell pellet in 5mL containing 20mM BaCl 2 Add 0.5g of glucose to normal saline, react in an ice-water bath for 2 hours, then centrifuge and wash to fully wash away the BaCl outside the cells 2 , collected the centrifuged cell pellet and resuspended in 5 mL containing 20 mM Na 2 CO 3 placed in a shaker (oscillating rate of 120rpm) at 25°C for 2 hours, then centrifuged and washed to obtain functionalized erythrocytes containing barium carbonate. The functionalized erythrocytes were pulverized, separated and purified to obtain erythrocytes with an average particle size of 4.2nm. Barium Carbonate Nanoparticles.

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Abstract

The invention discloses a preparation method of nano barium carbonate by using red blood cells as bioreactor, which belongs to the technical field of synthesis of inorganic nano materials. The technical proposal of the invention comprises the following main points that the upper plasma is removed from animal blood or human blood by centrifugation and the remainder is washed with physiological saline several times to obtain erythrocyte sedimentation, the erythrocyte sedimentation is added to the physiological saline containing soluble barium salt and the reaction is carried out under the condition of ice-water bath, then the mixture obtained by the reaction is centrifuged and washed after the reaction, the erythrocyte sedimentation obtained by the centrifugation is collected and resuspended in the physiological saline containing sodium carbonate to carry out the reaction, and functional red blood cells containing the barium carbonate are obtained by centrifugation after the reaction, then the barium carbonate nanoparticles with an average particle size of 4.2 nm are prepared by the crush, separation and purification of the functional red blood cells. The invention has a series of advantages of green environmental protection, simple operation, low cost and good biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of synthesis of inorganic nanometer materials, and in particular relates to a method for preparing nanobarium carbonate by using red blood cells as a bioreactor. Background technique [0002] At present, the existing chemical synthesis methods of inorganic nanomaterials have the disadvantages of poor stability and easy agglomeration; or environmental pollution, high synthesis cost, complex and difficult to control synthesis process, poor repeatability, and harsh reaction conditions. . With the development of science and technology, the focus of chemical synthesis has changed from traditional chemical methods to a new green synthesis method, and biosynthesis methods have emerged as the times require. Nano-biosynthesis technology mainly utilizes the biologically active molecules of organisms (such as proteins, polysaccharides, etc.) to synthesize new nanomaterials. It has the characteristics of low energy co...

Claims

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Application Information

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IPC IPC(8): C01F11/18B82Y40/00
CPCB82Y40/00C01F11/188C01P2002/72C01P2004/04C01P2004/64
Inventor 杨林谢晓晓陈会锋茹祥利
Owner HENAN NORMAL UNIV
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