Quick preparation method for candida albicans specimen for field emission scanning electron microscope

Abstract

The invention belongs to the bio-technical field, and particularly discloses a quick preparation method for a candida albicans specimen for field emission scanning electron microscope. The quick preparation method is characterized in that candida albicans thalluses are added into a glutaraldehyde fixing solution for being fixed; sterile deionized water is adopted to clean; and tertiary butanol with different concentration gradients is adopted for dehydration; a bacteria solution is dropped into pure tertiary butanol to freeze in a refrigerator of 4 DEG C; and after a dried specimen is sprayed with gold in an ion sputtering mode, the morphological structure of a thallus biological membrane can be observed through a scanning electron microscope by regulating and setting parameters. According to the quick preparation method for the candida albicans specimen for field emission scanning electron microscope provided by the invention, excreta of the candida albicans is reduced to affect image-forming quality of the specimen; a phenomenon that some samples are covered and shielded by phosphate particles is eliminated to the greatest extent; tiny deformation of the specimen is reduced and the biological membrane is prevented from dropping slightly; the surface of the thallus biological membrane is prevented from cracks or shrinkage sinking of varying degrees and even breakage; and meanwhile, damages on the surfaces of the thualuses are eliminated.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a rapid preparation method for Candida albicans field emission scanning electron microscope samples. Background technique [0002] The biofilm of Candida albicans is also called biofilm. In nature, the vast majority of bacteria exist in biofilms. Studies have shown that the existence of some Candida albicans biofilms is related to the severity of their pathogenesis. Therefore, exploring the biofilm structure of Candida albicans has gradually become a focus of scientific researchers. [0003] Scanning electron microscopy has become a powerful tool for studying the microscopic world, and many major discoveries and research results are obtained through observation. However, in the actual observation operation of the instrument, because the scanning electron microscope is a high-precision instrument, the structure is complex, and there are many factors that affect its imaging. In orde...

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Problems solved by technology

[0009] The traditional method uses different concentrations of gradient ethanol for dehydration, and the different dehydration time has a great influence on the surface structure of the sample. The observation results show that when the sample is dehydrated in a gradient of 10 minutes per step, the surface of the bacterial biofilm is cracked, or shrunk and sunken to varying degrees. is broken (as in Figure 4 ), and the damage

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