Adaptive optical focusing interference compensation method and system

Abstract

The invention discloses an adaptive optical focusing interference compensation method and system. Fluorescent light excited in a sample is received by a photomultiplier tube and the light intensity of a focusing center at this time is marked as a threshold; a digital micro-mirror device is arranged at a conjugate surface of a rear pupil surface of an objective lens, region division is carried out, each divided region reflects an incident light beam of the region to a side light path, so that the light after phase compensation can return to a front side light path and the light beams of other divided regions are kept to be unchanged; the photomultiplier tube records focusing center light intensities after light beam phase changes of the divided regions; the divided regions with the light intensities larger than threshold are marked to obtain a compensated divided region; and after the digital micro-mirror device loads the compensated divided region, the light beams are focused again and a light speckle with the higher central light intensity is formed in the sample. Therefore, on the basis of the optical interference principle, the quality of the focusing speckle inside the scattering medium can be improved rapidly; and a new idea is provided for the optogenetics and the living-body deep high-resolution optical microimaging technology.

Description

technical field [0001] The invention belongs to the field of optogenetics and optical microscopic imaging, and in particular relates to an adaptive optical focusing interference compensation method and system, and is applied to a new generation of non-invasive optogenetic light stimulation and in vivo deep penetration optical microscopic imaging . Background technique [0002] In the field of biomedical optics, optical scattering is the main factor restricting the quality of optical imaging. Most optical techniques for deep tissue imaging (eg, confocal laser imaging, two-photon microscopy, and optical coherence tomography) primarily utilize non-scattered photons (ie, ballistic photons) for imaging. The number of ballistic photons decays exponentially with depth, thus limiting the optical focus to a depth of 1 mm. [0003] Optogenetic technology requires photostimulation of specific neurons to study its neural circuit mechanism. However, the traditional optical fiber impla...

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Problems solved by technology

[0006] In order to solve the problems existing in the background technology, the purpose of the present invention is to use a digital micromirror device with a higher image refresh rate to solve the problem of spatial light modulation in traditional adaptive optogenetics The problem of taking a long time

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