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A kind of peptide-modified multi-wall carbon nanotube carrier and its preparation method and application

A technology of multi-wall carbon nanotubes and carriers, applied in the field of medicine, to achieve the effects of enhancing solubility, reducing toxicity of biological organisms, and simple operation

Active Publication Date: 2020-07-28
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] At present, there is no peptide-modified multi-walled carbon nanotube, and it can bind to unmethylated cytosine-guanine dinucleotide DNA (CpG DNA) and other immune activators to stimulate the body's anti-tumor immune effect, providing a new way for the prostate The treatment of cancer patients opens up a new treatment strategy, and also provides new theoretical basis and ideas for the application of carbon nanotubes in drug carriers

Method used

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  • A kind of peptide-modified multi-wall carbon nanotube carrier and its preparation method and application
  • A kind of peptide-modified multi-wall carbon nanotube carrier and its preparation method and application
  • A kind of peptide-modified multi-wall carbon nanotube carrier and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1: the synthesis of HR

[0055] 9-peptide HR amino acid sequence: His His His Arg Arg Arg Arg Arg Arg Arg (His, histidine; Arg, arginine) (SEQ ID NO: 1), HR, from Shanghai Jill Biochemical Co., Ltd. using the peptide solid-phase synthesis method It was synthesized and named as HR, and the synthesized HR was purified by preparative high-performance liquid chromatography to make its purity more than 95%. R is arginine, H is histidine, and the amino acids are connected by peptide bonds to form a 9-peptide.

Embodiment 2

[0056] Example 2: Preparation of peptide-modified multi-walled carbon nanotube carrier MHR

[0057] Carboxylation modification was carried out on the surface of multi-walled carbon nanotubes, and then the surface carboxyl groups were activated, and peptide solid-phase synthesis was carried out according to the sequence of HHHRRRRR (according to the mass ratio MWCNT / HR, 1:30), and the reaction was stirred for 24 hours. The carboxyl group and the amino group on histidine can be connected through an amide bond to obtain MHR, and then centrifuged at high speed to measure the electrical path of MHR, and then use a transmission electron microscope to investigate the morphology of the peptide-modified MHR, and use nuclear magnetic resonance spectroscopy and infrared spectroscopy to identify the obtained Structural features of polymers. The results of H NMR spectroscopy are shown in figure 1 , the infrared spectrum results see figure 2 , TEM of carboxylated carbon nanotubes and pep...

Embodiment 3

[0058] Example 3: In vitro transfection efficiency investigation of MHR / pEGFP

[0059] Select the mouse macrophage cell line RAW-264 (purchased from the Cell Culture Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences), add MHR-loaded EGFP plasmids with different nitrogen-to-phosphorus ratios (5, 10, 20, 40), and configure the nanocomposite vortex After spinning for 10 seconds, let it stand for 30 minutes, add it to the well plate, and after culturing for 4 hours, replace it with fresh medium, and continue culturing for 24 hours. Fluorescence microscopy to observe cell transfection, such as Figure 5 shown. Figure 5 It shows that the transfection efficiency of MHR is the best when N / P=10.

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Abstract

The invention relates to the technical field of medicines, and particularly relates to a peptide-modified multi-walled carbon nanotube carrier as well as a preparation method and application thereof. The invention provides the peptide-modified multi-walled carbon nanotube carrier which is formed by a nano-carrier MHR of a multi-walled carbon nanotube modified by short peptide composed of three arginines and six histidines. The nano-carrier provided by the invention can enhance solubility and dispersibility of carbon nanotubes in an aqueous solution, and reduce biological organism toxicity of the multi-walled carbon nanotubes; the prepared nano-carrier forms an MHR-CPG nano immune-activator by electrostatic adsorption of negatively charged immune-activator CpGDNA and the like; the nano-carrier provided by the invention can use MHR-CPG to stimulate the anti-tumor immune efficacy of the body, opens up a new treatment strategy for treatment of patients with prostate cancer, and also provides new theoretical basis and ideas for application of carbon nanotubes in drug carriers.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a peptide-modified multi-walled carbon nanotube carrier and its preparation method and application. Background technique [0002] Prostate cancer is one of the common malignant tumors that seriously threaten the health of men worldwide. According to the latest data from the American Cancer Society in 2016, prostate cancer ranks first in the estimated number of new cases, and ranks second in the estimated number of deaths (after lung cancer). At present, the treatment of advanced, recurrent, and metastatic prostate cancer is facing many problems. How to increase the body's anti-tumor immune effect has become a hot issue in related research. [0003] Tumor is one of the major diseases that seriously endanger human health. It is an important research topic in the medical field to find feasible and efficient tumor treatment methods and to completely conquer tumors. Following surge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K47/64A61K31/7084A61P35/00A61P37/04B82Y40/00B82Y5/00
CPCA61K31/7084A61K2039/55561B82Y5/00B82Y40/00
Inventor 高申夏清明宫春爱顾芬芬胡楚玲高原武鑫张丽娟田泾强磊
Owner SHANGHAI CHANGHAI HOSPITAL
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