Chitosan-platinum nano particle simulation oxidase

A technology of platinum nanometer and chitosan, which is applied in the nanometer field to achieve the effect of efficiently simulating the activity of oxidase, and the preparation process is simple and fast

Active Publication Date: 2017-09-15
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still relatively few reports on oxidase-like nanomaterials, mainly including nano-cerium oxide, nano-cobalt tetroxide, and nano-cobalt ferrite.

Method used

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  • Chitosan-platinum nano particle simulation oxidase
  • Chitosan-platinum nano particle simulation oxidase
  • Chitosan-platinum nano particle simulation oxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Weigh 0.1 g of chitosan and dissolve it in 50 mL of acetic acid with a concentration of 1% (v / v), and stir for 15 minutes to completely dissolve the chitosan to obtain a chitosan solution with a concentration of 0.2% (m / v). Add 2 mL of 10 mmol / L chloroplatinic acid to 47 mL of 0.2% (m / v) chitosan solution, stir for 30 minutes, and then add 1 mL of freshly prepared 0.2 mol / L chitosan solution dropwise. Sodium borohydride solution (completely added within 5 minutes), placed in the dark and stirred for 90 minutes to obtain a dark brown chitosan-platinum nanoparticle solution. Place chitosan-platinum nanoparticles and platinum nanoparticles without chitosan protection (i.e. bare platinum nanoparticles) at room temperature for 7 days, the results are as follows: figure 1 shown. As can be seen from the figure, after being placed at room temperature for 7 days, the chitosan-platinum nanoparticle solution was uniform and stable without precipitation (see figure 1 Panel A in t...

Embodiment 2

[0036] The chitosan-platinum nanoparticle solution prepared in Example 1 was drop-coated on a copper grid for transmission electron microscope testing. The results showed that the chitosan-platinum nanoparticles had an average particle size of 2.1 ± 0.3 nm (see figure 2 ). The interplanar spacing of the resulting nanoparticles is 0.224 nm, corresponding to the 111 crystal plane of the platinum crystal (see figure 2 illustration in ).

Embodiment 3

[0038] The chitosan-platinum nanoparticle solution obtained in Example 1 is freeze-dried to obtain powder, and the obtained powder is taken for elemental analysis (see image 3 ). The results showed that the obtained nanoparticles contained platinum element.

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Abstract

The invention discloses chitosan-platinum nano particle simulation oxidase. With chitosan used as a stabilizer and sodium borohydride used as a reducing agent, the chitosan-platinum nano particle simulation oxidase is synthesized through one step. The chitosan-platinum nano particle simulation oxidase can catalyze oxygen to oxidize 3,3',5,5'-tetramethyl benzidine hydrochloride to render color. The chitosan-platinum nano particle simulation oxidase has high affinity for the 3,3',5,5'-tetramethyl benzidine hydrochloride, and the Michaelis constant of the chitosan-platinum nano particle is 0.0179 mmol / L.

Description

technical field [0001] The invention relates to a platinum nano material with chitosan as a stabilizer and a preparation method thereof, which has the activity of simulating oxidase, and belongs to the field of nano technology. Background technique [0002] Enzymes have the functions of reducing the activation energy of chemical reactions, changing the reaction rate, and controlling the progress of reactions, and play a very important role in organisms and even in nature. Most natural enzymes are proteins with high efficiency and specificity, but their structure and function are easily affected by physical and chemical factors in the environment, and they need to be able to exert their activity at a specific pH and temperature. Compared with natural enzymes, nanomaterial-like enzymes have the advantages of simple preparation process, easy large-scale preparation, stable properties, reusable properties, strong tolerance to the external environment, and low manufacturing and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F1/02B22F9/24B82Y40/00
CPCB82Y40/00B22F9/24B22F1/07B22F1/16
Inventor 陈伟林秀玲邓豪华彭花萍刘爱林
Owner FUJIAN MEDICAL UNIV
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