Fermentation method of low volatile acid wine
A fermentation method and technology for sour wine, applied in the field of fermentation of low volatile acid wine, can solve problems such as reducing volatile acid, and achieve the effects of reducing the generation of volatile acid, reducing osmotic stress, and avoiding regulation and reaction
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Embodiment 1
[0058] This embodiment provides a fermentation method for low volatile acid wine (see the process figure 1 ), specifically including the following steps:
[0059] Fed-batch fermentation: Prepare 800ml of commercially available Chardonnay grape juice. The sugar concentration is 233.7g / L and the pH is 3.5. The grape juice is sugared by adding equal amounts of glucose and fructose. The concentration reaches 320g / L, add 250g / L(NH 4 ) 2 SO 4 Replenish nitrogen source and sterile filter for later use.
[0060] Take 50ml of the aseptically filtered grape juice in a 1L glass bottle; put 0.24g of dry yeast in 37℃ warm water, mix carefully, let it stand to rehydrate and activate for 20 minutes to obtain 50ml of yeast activation solution. Put the yeast activation liquid into a glass bottle and mix with grape juice with a sugar concentration of 320 g / L to obtain a mixed fermentation broth. At this time, the sugar concentration is diluted to 160 g / L. Fermentation is carried out in a constant t...
Embodiment 2
[0062] This embodiment provides a fermentation method that simulates low acidity wine (see the process figure 1 ), specifically including the following steps:
[0063] Fed-batch fermentation (fed-batch fermentation): prepare 800ml of simulated medium, whose ingredients are (g / L): D-glucose 175, D-fructose 175, Yeast Extract 1, citric acid 0.3 , Malic acid 3, tartaric acid 5, (NH 4 )SO 4 2. MgSO 4 0.4, KH 2 PO 4 5. Adjust pH=3.5 with 5mol / L NaOH, autoclave at 121℃ for 20min.
[0064] Take 50ml of the sterile filtered simulated grape juice into a 1L glass bottle, put 0.24g of dry yeast in 37℃ warm water, mix carefully, let it stand to rehydrate and activate for 20 minutes to obtain 50ml of yeast activation solution , Put the yeast activation liquid into a glass bottle and mix with a simulated medium with a sugar concentration of 350g / L to obtain a mixed fermentation broth. At this time, the sugar concentration is diluted to 175g / L. Fermentation is carried out in a constant temper...
Embodiment 3
[0066] This embodiment provides a fermentation method for low volatile acid wine (see the process figure 1 ), specifically including the following steps:
[0067] Fed-batch fermentation: Prepare 800ml of commercially available Chardonnay grape juice. The sugar concentration is 233.7g / L and the pH is 3.5. The grape juice is sugared by adding equal amounts of glucose and fructose. The concentration reaches 250g / L, add 250g / L (NH 4 ) 2 SO 4 Replenish nitrogen source and sterile filter for later use.
[0068] Take 80ml of the aseptically filtered grape juice into a 1L glass bottle; put 0.16g of dry yeast in 37℃ warm water, mix carefully, let it stand to rehydrate and activate for 30 minutes to obtain 50ml of yeast activation solution. Put the yeast activation liquid into a glass bottle and mix with grape juice with a sugar concentration of 250 g / L to obtain a mixed fermentation broth. At this time, the sugar concentration is diluted to 153.8 g / L. Fermentation is carried out in a const...
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