Chaetomium globosum LJ-S2L1 strain and application thereof

A technology of LJ-S2L1 and Chaetomium globosa, which is applied in the fields of application, fungi, biocides, etc., can solve the problems of unreported prevention and control of pepper wilt, does not involve isolation, and antagonizes the lack of species of Chaetomium to achieve growth The conditions are easy to control, the yield is large, and the effect of strong inhibition

Inactive Publication Date: 2017-09-15
贵州省植物保护研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few kinds of antagonistic chaetomes studied at present. Although two strains of Chaetomium globosa have been isolated and used to prevent and treat fungal diseases of ginseng and apples, Chinese patents: CN1046...

Method used

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  • Chaetomium globosum LJ-S2L1 strain and application thereof
  • Chaetomium globosum LJ-S2L1 strain and application thereof
  • Chaetomium globosum LJ-S2L1 strain and application thereof

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Embodiment 1

[0020] Embodiment 1 of the present invention: Screening of Chaetomium globosum LJ-S2L1 bacterial strain: select the capsicum plot that farmyard manure is applied continuously for more than two years after pepper fusarium wilt takes place seriously, apply the 7th day after farmyard manure decomposes, utilize The five-point method was used to collect the rhizosphere manure of healthy plants. Utilize the dilution separation method then, each soil sample takes by weighing 10g and puts into the Erlenmeyer flask that sterilized glass beads and 90mL sterile water are housed, vibrates at 160r / min for 30min to obtain 10 -1 sample suspension. Dilute the soil sample suspension with sterile water to 10 -2 、10 -3 , 10 -4 and 10 -5 different concentrations. Take 0.1mL of the soil suspension of the last three concentrations, disperse and drop it on the PDA plate, spread it evenly with a sterilized triangular glass rod, let it stand for 20 minutes, and then put it under the dark conditio...

Embodiment 2

[0028]Embodiment 2 of the present invention: Inhibitory effect of Chaetomium globosum LJ-S2L1 bacterial strain on pepper wilt pathogen

[0029] Use the plate confrontation method to screen bio-control bacteria and determine the inhibition rate: use a puncher to punch out the target pathogenic bacteria and the bacteria cakes to be tested with a diameter of 5mm, and connect the two cakes to the two sides of the same PDA plate with a distance of 5.5cm. Each group was repeated three times, with no test bacteria as the control, cultured at 25°C for 7 days, measuring the colony radius of the pathogenic bacteria towards the test bacteria, and calculating the inhibition rate of the test bacteria. The strains with obvious inhibitory effect on the pathogenic bacteria of Fusarium wilt of pepper were selected for re-screening. Growth inhibition rate (%)=(control colony diameter-treated colony diameter / control colony diameter-5mm)×100.

[0030] Validation of the inhibition zone method: us...

Embodiment 3

[0035] Embodiment 3 of the present invention: Inhibitory effect of fermentation liquid of Chaetomium globosum LJ-S2L1 strain on pepper wilt pathogen

[0036] Fermentation broth preparation: Inoculate the activated strain LJ-S2L1 into PDB medium, culture at 160r / min at 25°C for 7 days, filter the bacterial fluid with double-layer sterile gauze, and then put the filtrate into a centrifuge at 10,000r / min After 3 minutes, the obtained supernatant was divided into two parts, one was subjected to high-pressure sterilization, and the other was not subjected to any treatment. Using Fusarium wilt of capsicum as indicator bacteria, the following two methods were used to determine the antibacterial properties of the two fermentation broths of strain LJ-S2L1.

[0037] Colony diameter method: Add the two fermentation broths of the strain LJ-S2L1 to the PDA medium cooled to about 50°C at a ratio of 1:4 in volume, mix well to make a plate containing the fermentation broth of biocontrol bacte...

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Abstract

The invention discloses a chaetomium globosum LJ-S2L1 strain and an application thereof. The preservation number of the strain is CCTCCM 2016649. The strain is simple in culture requirement, high in yield and good in antibacterial effect, growth conditions are easily controlled, and capsicum fusarium wilt pathogenic bacterium hyphae can excessively shrink, bend and partially expand through the strain. Toxicity measurement results show that the ratio of fermentation liquor of the strainS2L1 for inhibiting capsicum fusarium wilt pathogenic bacteria can reach 72.10% in 1000 micrograms/mL, median inhibiting concentration is 300.63 micro-liters/mL, and pot experiment control effect can reach 71.8% and is equal to that of 500 times dilution of 80% of carbendazim. The strain has wide application prospect in the prevention aspect of the capsicum fusarium wilt, both the fermentation liquor and sterile fermentation filtering liquid of the strain have high inhibiting effect, and the strain can serve as biological agents for the capsicum fusarium wilt.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chaetomium globosa LJ-S2L1 strain and application thereof. Background technique [0002] Fusarium wilt is a serious soil-borne disease in pepper production in recent years. The pathogenic bacteria Fusarium oxysporum not only has a wide range of hosts, but also can survive in the soil for a long time. With the expansion of the planting area of ​​pepper and the improvement of the multiple cropping index, Fusarium wilt of pepper accumulates and spreads in the field year by year, resulting in the widespread occurrence of the disease, and in severe cases, the yield can be reduced by 70% to 80%. Not only that, the occurrence of fusarium wilt weakens the immunity of the pepper plant itself after the disease, making it easier for other soil-borne pathogens to invade, and even causes the whole field of pepper to wither and die when the disease is severe. [0003] The current control method...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A61P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 卯婷婷陶刚赵玳琳顾金刚
Owner 贵州省植物保护研究所
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