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Xylaria fungus strain E68 and application thereof

A technology of the genus Anthracera and fungi, which is applied in the fields of fungi, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of pregnant women who are prone to miscarriage, the content exceeds the safe value, and destroys the natural environment, and achieves Important industrial application value, good market prospect, easy industrialization effect

Active Publication Date: 2017-09-19
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that excessive herbicide residues in plants can cause various diseases such as cancer, leukemia, kidney necrosis and abortion in pregnant women through eating, and can also cause abortion or even death in animals; the abundance of herbicides in the environment A large amount of soil and water are polluted and destroyed by herbicides, and their content exceeds the safe value; the herbicides left in the soil cause phytotoxicity of subsequent crops, which in turn leads to reduced production, and severe areas even cause production failure. In some areas, soil compaction due to the use of herbicides is no longer suitable for farming

Method used

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  • Xylaria fungus strain E68 and application thereof
  • Xylaria fungus strain E68 and application thereof
  • Xylaria fungus strain E68 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 : Isolation, screening and identification of Xylaria sp. E68

[0021] 1.1 Experimental materials

[0022] Inorganic salt medium: 1 g of ammonium nitrate, 1.5 g of dipotassium hydrogen phosphate, 0.5 g of potassium dihydrogen phosphate, 0.1 g of crystalline magnesium sulfate, 45.72 mg of ferrous sulfate (7 water), 1 L of water, 40 g of agar.

[0023] LB medium: 10g tryptone, 5g yeast powder, 10g sodium chloride, 1L water, 40g agar.

[0024] PDA medium: 200g potatoes, 20g glucose, 500-600mL water, boiled for about half an hour, passed through gauze, dilute to 1 liter, add 40g agar.

[0025] The degradation solution is a mixture of inorganic salt medium and dimethyltetrachloride.

[0026] 1.2 Separation and identification methods

[0027] Xylaria sp. E68 of the present invention is obtained from Mingyang, Guangxi sampling, isolation and screening, and is preserved by the Guangdong Microbial Culture Collection Center, and its preservation number is GDMCC No.6...

Embodiment 2

[0041] Example 2 : Study on the Effect of Xylaria sp. E68 on the Degradation of Dimethyltetrachloride

[0042] 2.1 Cultivation of degrading bacteria

[0043] Prepare PDB medium, sterilize it, and put it in sterilized 30mL vials with caps, 10mL per bottle, scrape the purified fungal hyphae in PDA medium with an inoculation loop, and insert them into PDA medium vials respectively, To obtain expanded cultured cells, the culture condition is to cultivate at 28°C for 3-4 days and wait for use.

[0044] 2.2 Screening of degrading bacteria

[0045] Prepare the degradation test medium of degrading bacteria, and distribute it in sterile 30mL vials with caps, 10mL per bottle. Add 0.55g / L (by dry weight) to expand the cultured cells and mix with the subpackaged degrading bacteria degradation test medium. Before mixing, wash with the degradation bacteria degradation test medium for 3 times and then mix with the degradation test medium , three parallel treatments and a blank control w...

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Abstract

The invention belongs to the technical field of plant endophyte degradation pesticides and particularly relates to a Xylaria fungus strain E68 and application thereof in the degradation of a herbicide 2-methyl-4-chlorophenoxy acetic acid. The Xylaria fungus strain E68 is taxonomically named as Xylaria sp. E68 and is preserved in Guangdong Microbial Culture Collection Center on April 19th, 2017, and the preservation number of the Xylaria sp. E68 is GDMCC No. 60162. The Xylaria sp. E68 can degrade the herbicide 2-methyl-4-chlorophenoxy acetic acid in soil or water and is low in cost, easy to achieve industrialization, promising in market prospect and high in industrial application value.

Description

technical field [0001] The invention belongs to the technical field of plant endophytic bacteria degrading pesticides, and specifically relates to a fungus strain E68 of the genus Anthracera and its application in degrading the herbicide dimethyltetrachloride. Background technique [0002] With the development of China's agricultural modernization, changes in the structure of agricultural planting, the development of agricultural management models in the direction of scale, industrialization, and intensification, and the reduction of rural labor force, the demand for herbicides in agriculture will continue to increase. At the same time, changes in agricultural cultivation methods have also contributed to a surge in the demand for herbicides. [0003] The use of herbicides has greatly increased crop yields, reduced the burden on farmers, and provided a guarantee for agricultural labor-saving and mechanized production. At the same time, the use of herbicides has also had a ser...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C02F3/34C02F101/36C12R1/645
CPCB09C1/105C02F3/347C02F2101/306C02F2101/36C12N1/145C12R2001/645
Inventor 王彦辉封国君李红红曾东强唐文伟
Owner GUANGXI UNIV