Loach protein polypeptide and application thereof

A technology of fish protein and loach, which is applied in the field of bioengineering, can solve problems such as the lack of reports on rich protein resources, and achieve the effects of extending the loach industry chain, high purity, and high yield

Inactive Publication Date: 2017-09-19
深圳知本康业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on loach mainly focuses on loach polysaccharides, trace elements, etc., but there are few reports on the rich protein resources in loach.

Method used

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  • Loach protein polypeptide and application thereof
  • Loach protein polypeptide and application thereof

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preparation example Construction

[0051] A loach fish protein polypeptide is a loach fish antihypertensive peptide with a molecular weight between 1000-1300Da. The loach fish antihypertensive peptide is prepared by microwave-assisted enzymolysis technology. The preparation method includes the following steps:

[0052] Preprocessing steps:

[0053] Keep fresh loach in clean water for 24 hours, remove the head and viscera, and use a meat grinder to grind it into a puree;

[0054] Steps for protein modification:

[0055] Weigh loach meat paste, add pure water according to the solid-to-liquid ratio of 1:20, then adjust the pH value to 3.0-5.0 with hydrochloric acid, and treat it with microwave for 20 minutes at 40-50°C, the frequency of microwave is 2500MHz, and the power is 15KW; Then keep it warm for 30-45 minutes, keep the pH value below 5.0, and obtain the modified loach fish protein solution; preferably, adjust the pH value to 3.5 with hydrochloric acid, and treat with microwave at 45°C.

[0056] Steps for ...

Embodiment 1

[0090] A loach fish protein polypeptide is a loach fish antihypertensive peptide with a molecular weight between 1000-1300Da. The loach fish antihypertensive peptide is prepared by microwave-assisted enzymolysis technology. The preparation method includes the following steps:

[0091] Preprocessing steps:

[0092] Keep fresh loach in clean water for 24 hours, remove the head and viscera, and use a meat grinder to grind it into a puree;

[0093] Steps for protein modification:

[0094] Weigh loach meat paste, add pure water according to the solid-to-liquid ratio of 1:20, then adjust the pH value to 3.5 with hydrochloric acid, treat with microwave for 20 minutes at 45°C, the frequency of microwave is 2500MHz, and the power is 15KW; after that, keep warm for 40min, The pH value is kept below 5.0 to obtain a modified loach fish protein solution;

[0095] Steps for an enzymatic hydrolysis:

[0096] Use the modified loach fish protein solution as the substrate, adjust the pH valu...

Embodiment 2

[0108] A loach fish protein polypeptide is a loach fish antihypertensive peptide with a molecular weight between 1000-1300Da. The loach fish antihypertensive peptide is prepared by microwave-assisted enzymolysis technology. The preparation method includes the following steps:

[0109] Preprocessing steps:

[0110] Keep fresh loach in clean water for 24 hours, remove the head and viscera, and use a meat grinder to grind it into a puree;

[0111] Steps for protein modification:

[0112] Weigh the loach meat paste, add pure water according to the solid-to-liquid ratio of 1:20, then adjust the pH value to 3.0 with hydrochloric acid, treat it with microwave for 20 minutes at 40°C, and then keep it warm for 30 minutes, keeping the pH value below 5.0 to obtain the modification Loach fish protein solution;

[0113] Steps for an enzymatic hydrolysis:

[0114] Use the modified loach fish protein solution as the substrate, adjust the pH value to 3.0 with hydrochloric acid, add the com...

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Abstract

The invention discloses a loach protein polypeptide and application thereof. The loach protein polypeptide is an antihypertensive peptide with the molecular weight being 1000-1300Da, and the antihypertensive peptide is prepared by the microwave-assisted enzymolysis technology. A preparation method of loach protein polypeptide includes: preprocessing, modifying protein, performing primary enzymolysis, performing secondary enzymolysis, deactivating enzymes, separating and purifying, performing high-pressure steam sterilizing, and concentrating and drying. By improving the enzymolysis process and technology of the loach protein polypeptide and using the microwave-assisted enzymolysis technology, the polypeptide with antihypertensive activity is prepared. The prepared loach protein polypeptide is high in yield and purity, can be used as the additive or active drug to be added to various industry and fields such as human food, drugs and healthcare products, and is significant to loach industrial chain prolonging, comprehensive increasing of loach industry comprehensive benefits, agriculture efficiency increasing, farmer income increasing, countryside prosperity and the promotion of one belt and one road construction.

Description

technical field [0001] The invention relates to a bioengineering technology, in particular to a loach fish protein polypeptide and its application. Background technique [0002] Loach (Misgurnus anguillicaudatus), also known as loach and loach, is a small fish belonging to Cyprinidae Cyprinidae and Loach family. It is distributed in fresh water all over my country, with large natural output and wide distribution. It is a common aquatic product. Class public food. Loach meat is tender, delicious, nutritious, and has important medicinal value. It is recorded in detail in Chinese medicine books such as "Compendium of Materia Medica" and "Dictionary of Traditional Chinese Medicine". The synovial fluid secreted by the whole loach or the skin can be used as medicine. The effect of invigorating qi and dispelling dampness can also cure thirst, edema and jaundice, clear away heat and detoxify, hemorrhoids, impotence, etc. It also has a significant effect on improving liver function i...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/16C07K1/14C12G3/04A61K38/01A61P9/12A61K36/24
CPCA61K36/24A61K38/012C07K1/14C07K1/16C12G3/04C12P21/06A61K2300/00
Inventor 不公告发明人
Owner 深圳知本康业有限公司
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