Preparation method of molecular imprinting sensor for detecting anthocyanin

A technology of molecular imprinting and anthocyanin, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as poor regeneration and reversibility, high detection limit, slow electron transfer speed and response, and achieve high affinity and selectivity Sexuality, the effect of response improvement

Inactive Publication Date: 2017-09-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of graphene-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with double distilled water, dry it, and drop 13 μL of graphite oxide on the surface of the glassy carbon electrode Graphene is a 0.3g / L N,N-dimethylformamide dispersion, placed under an infrared lamp, and after the solvent is evaporated, a graphene-modified glassy carbon electrode is obtained;

[0023] (2) Preparation of anthocyanin molecularly imprinted polymer: In the reactor, add deionized water: 17mL, absolute ethanol: 68mL, ethylene glycol dimethacrylate: 8g, aconitic acid: 6g, 1-propenyl-3-methylimidazolium bromide: 12g, azobisisoheptanonitrile: 1.5g, anthocyanin: 1.0g, stir and dissolve, deoxygenate with inert gas for 20 min, anaerobic atmosphere, 55±2 Stir and react at ℃ for 13 hours, soak the obtained product in a mixed solution of ethanol: acetic acid with a volume ratio of 8:1 for 7 ho...

Embodiment 2

[0026] (1) Preparation of graphene-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with double distilled water, dry it, and drop 12 μL of graphite oxide on the surface of the glassy carbon electrode Graphene is a 0.3g / L N,N-dimethylformamide dispersion, placed under an infrared lamp, and after the solvent is evaporated, a graphene-modified glassy carbon electrode is obtained;

[0027] (2) Preparation of anthocyanin molecularly imprinted polymer: In the reactor, add deionized water: 15mL, absolute ethanol: 80mL, ethylene glycol dimethacrylate: 6g, aconitic acid: 5g, 1-propenyl-3-methylimidazolium bromide: 10g, azobisisoheptanonitrile: 2g, anthocyanin: 2g, stir and dissolve, deoxygenate with inert gas for 20 min, in an oxygen-free atmosphere, stir at 55±2°C After reacting for 14 hours, soak the obtained product in a mixed solution of ethanol: acetic acid with a volume ratio of 8:1...

Embodiment 3

[0030] (1) Preparation of graphene-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, dry it, and drop 15 μL of graphite oxide on the surface of the glassy carbon electrode Graphene is a 0.3g / L N,N-dimethylformamide dispersion, placed under an infrared lamp, and after the solvent is evaporated, a graphene-modified glassy carbon electrode is obtained;

[0031] (2) Preparation of anthocyanin molecularly imprinted polymer: In the reactor, add deionized water: 20mL, absolute ethanol: 63mL, ethylene glycol dimethacrylate: 10g, aconitic acid: 8g, 1-propenyl-3-methylimidazolium bromide: 11g, azobisisoheptanonitrile: 1.0g, anthocyanin: 0.5g, stir and dissolve, deoxygenate with inert gas for 20 min, anaerobic atmosphere, 55±2 Stir and react at ℃ for 12 h, soak the obtained product in a mixed solution of ethanol: acetic acid with a volume ratio of 8:1 for 8 h, remov...

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Abstract

The invention discloses a preparation method of a molecular imprinting sensor for detecting anthocyanin. The preparation method is characterized by including the steps: firstly, modifying a glassy carbon electrode by the aid of graphene oxide; secondly, adding 15-20% of deionized water, 50-60% of absolute ethyl alcohol, 6-10% of ethylene glycol dimethacrylate, 5-8% of aconitic acid, 10-15% of 1-allyl-3-methyl imidazole bromide, 1.0-3% of azobisisoheptonitrile and 0.2-2.0% of anthocyanin into a reactor, performing reaction for 12-15h in a stirring manner at the temperature of 55+/-2 DEG C under oxygen-free atmosphere, soaking a product for 6-8h by the aid of mixed solution of ethyl alcohol and acetic acid, removing template molecules, and performing drying to obtain an anthocyanin molecular imprinting polymer; finally, dispensing the anthocyanin molecular imprinting polymer on modified graphene oxide to obtain the molecular imprinting sensor of the anthocyanin. The volume ratio of the ethyl alcohol to the acetic acid is 8:1. The sensor has high affinity and selectivity, is high in sensitivity, good in specificity and rapid in detection and can be repeatedly used.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to the preparation of a molecularly imprinted sensor for detecting anthocyanins, specifically based on the molecular imprinted specific recognition, for detecting Anthocyanin technology in pharmaceuticals, food, and biological samples. Background technique [0002] Anthocyanidin, also known as anthocyanin, has a molecular formula of C15H11O6 and a relative molecular mass of 287. It is a kind of water-soluble natural pigment widely present in plants in nature, and belongs to flavonoid compounds. It is also the main coloring substance in plant petals, and most of the colorful colors of fruits, vegetables, and flowers are related to it. [0003] Anthocyanins exist in the vacuoles of plant cells and can be converted from chlorophyll. Under the condition of different pH value of plant cell vacuoles, ...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/308G01N27/48
Inventor 李慧芝翟玉博李志英
Owner UNIV OF JINAN
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