Preparation method of molecular imprinting sensor for detecting anthocyanin
A technology of molecular imprinting and anthocyanin, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as poor regeneration and reversibility, high detection limit, slow electron transfer speed and response, and achieve high affinity and selectivity Sexuality, the effect of response improvement
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Embodiment 1
[0022] (1) Preparation of graphene-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with double distilled water, dry it, and drop 13 μL of graphite oxide on the surface of the glassy carbon electrode Graphene is a 0.3g / L N,N-dimethylformamide dispersion, placed under an infrared lamp, and after the solvent is evaporated, a graphene-modified glassy carbon electrode is obtained;
[0023] (2) Preparation of anthocyanin molecularly imprinted polymer: In the reactor, add deionized water: 17mL, absolute ethanol: 68mL, ethylene glycol dimethacrylate: 8g, aconitic acid: 6g, 1-propenyl-3-methylimidazolium bromide: 12g, azobisisoheptanonitrile: 1.5g, anthocyanin: 1.0g, stir and dissolve, deoxygenate with inert gas for 20 min, anaerobic atmosphere, 55±2 Stir and react at ℃ for 13 hours, soak the obtained product in a mixed solution of ethanol: acetic acid with a volume ratio of 8:1 for 7 ho...
Embodiment 2
[0026] (1) Preparation of graphene-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with double distilled water, dry it, and drop 12 μL of graphite oxide on the surface of the glassy carbon electrode Graphene is a 0.3g / L N,N-dimethylformamide dispersion, placed under an infrared lamp, and after the solvent is evaporated, a graphene-modified glassy carbon electrode is obtained;
[0027] (2) Preparation of anthocyanin molecularly imprinted polymer: In the reactor, add deionized water: 15mL, absolute ethanol: 80mL, ethylene glycol dimethacrylate: 6g, aconitic acid: 5g, 1-propenyl-3-methylimidazolium bromide: 10g, azobisisoheptanonitrile: 2g, anthocyanin: 2g, stir and dissolve, deoxygenate with inert gas for 20 min, in an oxygen-free atmosphere, stir at 55±2°C After reacting for 14 hours, soak the obtained product in a mixed solution of ethanol: acetic acid with a volume ratio of 8:1...
Embodiment 3
[0030] (1) Preparation of graphene-modified glassy carbon electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, dry it, and drop 15 μL of graphite oxide on the surface of the glassy carbon electrode Graphene is a 0.3g / L N,N-dimethylformamide dispersion, placed under an infrared lamp, and after the solvent is evaporated, a graphene-modified glassy carbon electrode is obtained;
[0031] (2) Preparation of anthocyanin molecularly imprinted polymer: In the reactor, add deionized water: 20mL, absolute ethanol: 63mL, ethylene glycol dimethacrylate: 10g, aconitic acid: 8g, 1-propenyl-3-methylimidazolium bromide: 11g, azobisisoheptanonitrile: 1.0g, anthocyanin: 0.5g, stir and dissolve, deoxygenate with inert gas for 20 min, anaerobic atmosphere, 55±2 Stir and react at ℃ for 12 h, soak the obtained product in a mixed solution of ethanol: acetic acid with a volume ratio of 8:1 for 8 h, remov...
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