A kind of preparation method of blood lipid-regulating active polysaccharide
A technology for regulating blood lipids and polysaccharides is applied in the field of preparation of blood lipid-regulating active polysaccharides to achieve the effects of reducing triglycerides and inhibiting pancreatic lipase
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Embodiment 1
[0024] Embodiment 1: the extraction of inhibitor
[0025] The fresh red hair algae was purchased from Putian, Fujian. The extraction is carried out by water extraction and alcohol precipitation method, and the specific operation is as follows: clean the fresh red hair algae, dry them in an oven at 50°C, and then pulverize them with a pulverizer. Add 3 to 5 times the volume of methanol to the dried algae powder, stir continuously, and wash repeatedly three times to ensure sufficient decolorization, and soak overnight. After soaking, centrifuge at 4000r / min for 15 minutes, take out the precipitate, add distilled water and heat it in a 90°C water bath for 2 hours, centrifuge at 4000r / min for 20 minutes, take the supernatant, repeat the hot water extraction of the precipitate for 2 hours and then centrifuge, and combine the supernatants of the two centrifuges Liquid, concentrated by evaporation, the working temperature is 50°C, and the extract is concentrated to 1 / 10 of the origi...
Embodiment 2
[0027] Example 2: Gel Filtration Purification of Polysaccharides
[0028] The crude polysaccharide was purified by Sephadex G75 gel column (C16 / 100), and the specific operation was as follows: the concentration of the sugar solution was 5 mg / mL, the sample volume was 3 mL, and eluted with 0.1 M NaCl solution. Use an automatic collector to receive, receive 50 tubes, each tube is 5mL. Use the phenol sulfuric acid method to track and detect the elution at 490nm. Take the tube number as the abscissa and the absorbance value as the ordinate to make an elution diagram. The eluted peaks were combined, desalted by dialysis, and freeze-dried to obtain a preliminary purified polysaccharide F. The result is as figure 1 As shown, the elution curve of the crude polysaccharide of red hair algae obtained a single symmetrical peak. Since the crude polysaccharide was deproteinized before purification, a single component was eluted, which was named F, indicating that in the F component It is...
Embodiment 3
[0029] Example 3: Anion Exchange Chromatography of Inhibitor F
[0030] The DEAE Cellulose 52 gel column was used to further purify the initially purified polysaccharide F. The specific operation was as follows: the sample was prepared into a 3mg / mL solution, and the sample volume was 5mL, and 0M (distilled water), 0.1M, 0.3M, 0.5M Concentration of NaCl solution gradient elution, each eluate, collect 40 tubes, each tube collected 5mL. Also track and monitor with the phenol-sulfuric acid method, take the tube number as the abscissa, and the absorbance value as the ordinate, and make an elution diagram. The eluted peaks were combined, desalted by dialysis, and freeze-dried to obtain purified polysaccharides SF1, SF2, and SF3. The result is as
[0031] figure 2 As shown, when eluted with distilled water, there is no elution peak, but when the concentration of NaCl solution is 0.1, 0.3, and 0.5M, there is an elution peak, named SF1, SF2, and SF3 respectively.
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