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A kind of preparation method of blood lipid-regulating active polysaccharide

A technology for regulating blood lipids and polysaccharides is applied in the field of preparation of blood lipid-regulating active polysaccharides to achieve the effects of reducing triglycerides and inhibiting pancreatic lipase

Active Publication Date: 2021-05-25
珠海横琴澳瑞医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the side effects of western medicine, such as abdominal pain, constipation, flatulence, fatigue, headache, etc. have gradually attracted people's attention

Method used

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  • A kind of preparation method of blood lipid-regulating active polysaccharide
  • A kind of preparation method of blood lipid-regulating active polysaccharide
  • A kind of preparation method of blood lipid-regulating active polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the extraction of inhibitor

[0025] The fresh red hair algae was purchased from Putian, Fujian. The extraction is carried out by water extraction and alcohol precipitation method, and the specific operation is as follows: clean the fresh red hair algae, dry them in an oven at 50°C, and then pulverize them with a pulverizer. Add 3 to 5 times the volume of methanol to the dried algae powder, stir continuously, and wash repeatedly three times to ensure sufficient decolorization, and soak overnight. After soaking, centrifuge at 4000r / min for 15 minutes, take out the precipitate, add distilled water and heat it in a 90°C water bath for 2 hours, centrifuge at 4000r / min for 20 minutes, take the supernatant, repeat the hot water extraction of the precipitate for 2 hours and then centrifuge, and combine the supernatants of the two centrifuges Liquid, concentrated by evaporation, the working temperature is 50°C, and the extract is concentrated to 1 / 10 of the origi...

Embodiment 2

[0027] Example 2: Gel Filtration Purification of Polysaccharides

[0028] The crude polysaccharide was purified by Sephadex G75 gel column (C16 / 100), and the specific operation was as follows: the concentration of the sugar solution was 5 mg / mL, the sample volume was 3 mL, and eluted with 0.1 M NaCl solution. Use an automatic collector to receive, receive 50 tubes, each tube is 5mL. Use the phenol sulfuric acid method to track and detect the elution at 490nm. Take the tube number as the abscissa and the absorbance value as the ordinate to make an elution diagram. The eluted peaks were combined, desalted by dialysis, and freeze-dried to obtain a preliminary purified polysaccharide F. The result is as figure 1 As shown, the elution curve of the crude polysaccharide of red hair algae obtained a single symmetrical peak. Since the crude polysaccharide was deproteinized before purification, a single component was eluted, which was named F, indicating that in the F component It is...

Embodiment 3

[0029] Example 3: Anion Exchange Chromatography of Inhibitor F

[0030] The DEAE Cellulose 52 gel column was used to further purify the initially purified polysaccharide F. The specific operation was as follows: the sample was prepared into a 3mg / mL solution, and the sample volume was 5mL, and 0M (distilled water), 0.1M, 0.3M, 0.5M Concentration of NaCl solution gradient elution, each eluate, collect 40 tubes, each tube collected 5mL. Also track and monitor with the phenol-sulfuric acid method, take the tube number as the abscissa, and the absorbance value as the ordinate, and make an elution diagram. The eluted peaks were combined, desalted by dialysis, and freeze-dried to obtain purified polysaccharides SF1, SF2, and SF3. The result is as

[0031] figure 2 As shown, when eluted with distilled water, there is no elution peak, but when the concentration of NaCl solution is 0.1, 0.3, and 0.5M, there is an elution peak, named SF1, SF2, and SF3 respectively.

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Abstract

The invention discloses a method for extracting blood-lipid-regulating active polysaccharides from red hair algae, which comprises the following steps: step 1, extraction: fresh red hair algae are cleaned, dried, and crushed to obtain algae powder, added with distilled water, and extracted Concentrate to obtain a concentrated solution; Step 2, alcohol precipitation: add ethanol dropwise, centrifuge, take the precipitate, and freeze-dry to obtain a crude polysaccharide; Step 3, gel chromatography: dissolve the crude polysaccharide in distilled water, load the sample, wash Remove, track and detect, collect elution peaks, dialyze and desalt, and freeze-dry to obtain preliminary purified polysaccharides; Step 4, ion exchange chromatography: dissolve the preliminary purified polysaccharides in distilled water, load samples, elute, track and monitor, collect The peak is eluted, desalted by dialysis, and freeze-dried to obtain a purified polysaccharide, that is, a polysaccharide with blood lipid regulating activity. The active polysaccharide prepared by the invention is a natural blood lipid-regulating substance, and its blood-lipid regulating effect is obvious.

Description

technical field [0001] The invention relates to the technical field of active polysaccharides, in particular to a preparation method of blood lipid-regulating active polysaccharides. Background technique [0002] Blood lipids refer to lipids in the blood, including neutral fats and lipids, in which triglycerides and cholesterol belong to neutral fats, and phospholipids, sterols, and steroids belong to lipids. They are widely present in the human body and are essential substances for the basic metabolic functions of human life cells. Hyperlipidemia is a type of systemic disease in which the metabolism or transport of lipids in the human body is abnormal, causing the content of one or more lipids in the blood to exceed the normal range. [0003] With the development of modern medicine, the pathogenesis and effects of hyperlipidemia have been more deeply understood. Therefore, it is of great significance to study practical and effective lipid-lowering methods for the preventio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P3/06
CPCC08B37/0003
Inventor 姜泽东余刚宋田源倪辉杨远帆李利君朱艳冰杜希萍蔡慧农
Owner 珠海横琴澳瑞医疗科技有限公司
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