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A system and method for measuring the antioxidant capacity of humic substances

A technology of anti-oxidation ability and humus, applied in the field of environmental electrochemistry, can solve the problems of storage time impact, high energy consumption, overestimation of EDC, etc.

Active Publication Date: 2020-03-06
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Among them, the chemical method has the following disadvantages: First, in the measurement, Zn and H 2 O will react to form H 2 , while H 2 itself is a reducing agent, thereby overestimating the EDC of HSs; secondly, the reaction rate of HSs with chemical reducing agents or oxidizing agents is very slow, and HSs and K 3 [Fe(CN) 6 ] The reaction can reach equilibrium after 24h, so it is difficult to determine whether the measured EDC is exactly the value to reach equilibrium; finally, the use of chemical reagents may cause side reactions of HSs, such as complexation or covalent modification, etc., Changed the chemical composition or structure of HSs, thus causing errors in the measurement
Compared with the antioxidant capacity measured by chemical methods, the electrochemical method has the following advantages: HSs is rapidly oxidized at the potential of the working electrode, and the method is simple and fast; the working electrode is used as the acceptor for the oxidation of HSs to generate electrons, which avoids possible damage caused by chemical reagents. However, it also has certain defects: First, in order to ensure the accuracy of EDC measurement, it is necessary to control the constant potential with the help of an electrochemical workstation, and the economic investment and energy consumption of supporting instruments are large; secondly, it cannot be realized Real-time measurement, while the physical and chemical properties of HSs samples taken outdoors will change with time or geographical changes, and longer storage time will affect the true EDC of HSs

Method used

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  • A system and method for measuring the antioxidant capacity of humic substances
  • A system and method for measuring the antioxidant capacity of humic substances
  • A system and method for measuring the antioxidant capacity of humic substances

Examples

Experimental program
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Effect test

Embodiment 1

[0083] (1) System for measuring the antioxidant capacity of humic substances:

[0084] see Figure 2~3 , figure 2 It is a schematic diagram of the structure and working process of the system for measuring the antioxidant capacity of humic substances provided in Example 1 of the present invention, wherein, 1 is an anode chamber, 2 is an anode chamber electrolyte, 3 is an anode chamber electrode, 4 is a cathode chamber, and 5 is a cathode Chamber electrolyte, 6 is the cathode chamber electrode, 7 is the proton exchange membrane, 8 is the load resistance, and 9 is the voltmeter; image 3 The physical picture of the system for measuring the antioxidant capacity of humic substances provided in Example 1 of the present invention.

[0085]The anode chamber and cathode chamber of the system are made of plexiglass, and the overall size of the two is 40mm×40mm×60mm; the anode chamber and cathode chamber are both cylindrical sealed anaerobic structures, with an inner diameter of 15mm ...

Embodiment 2

[0095] (1) System for measuring the antioxidant capacity of humic substances:

[0096] Using the system provided in Example 1, the difference is that: the electrolyte in the anode chamber is a phosphate buffer solution containing 0.1M KCl, that is, no ABTS is added;

[0097] (2) The method for measuring the antioxidant capacity of humic substances:

[0098] Humus solution to be tested: prepare AHA into a 2g / L aqueous solution, adjust the pH to 7, and expose to N before use 2 Deoxygenation for 30 minutes;

[0099] Add 3mL of phosphate buffer solution containing 0.1M KCl to the anode chamber, cover the soft plug to seal, at the same time, add 3mL cathode chamber electrolyte to the cathode chamber, cover the soft plug to seal; wait for the voltammeter data to be stable, connect the external load resistance (R=1000Ω ), the voltage value of the voltammeter was stable again (U≈700mV), and 5 μL, 10 μL, 20 μL, 40 μL, 60 μL, and 80 μL of the humic substances to be tested were added t...

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Abstract

The invention provides a system and a method for determining the anti-oxidization capability of humus. The system for determining the anti-oxidization capability of the humus comprises an anode chamber, a cathode chamber, a proton exchange membrane, a load resistor and a voltmeter, wherein the anode chamber is provided with anode electrolyte and an anode chamber electrode; the cathode chamber is provided with cathode electrolyte and a cathode chamber electrode; the proton exchange membrane is used for separating the anode chamber and the cathode chamber; the load resistor is externally connected with the anode chamber electrode and the cathode chamber electrode; the voltmeter is connected with the load resistor in parallel; the anode electrolyte comprises a phosphoric acid buffering solution containing potassium chloride; the cathode electrolyte is an acidic potassium permanganate solution. Compared with the prior art, the system for determining the anti-oxidization capability of the humus, provided by the invention has the characteristics that a matched instrument of an electrochemical method is simplified on the basis of guaranteeing the accuracy and outdoor sample measurement can be met, so that rapid and real-time determination of the anti-oxidization capability of the humus is realized, the accuracy is high, the cost is low and the energy consumption is small.

Description

technical field [0001] The invention relates to the technical field of environmental electrochemistry, and more specifically, relates to a system and method for measuring the anti-oxidation ability of humic substances. Background technique [0002] Humic Substances (HSs for short) are a class of polymer compounds that are transformed from animal and plant residues in water and soil through long-term physical, chemical, and biological actions. Under anaerobic conditions, HSs can act as an electron mediator to accept electrons from microbial respiration, and has the characteristics of accelerating electron shuttle between microorganisms and extracellular electron acceptors; under aerobic conditions, HSs can release electrons and react with oxidative substances , thus possessing antioxidant properties. The antioxidant properties of HSs are mainly due to the phenols in their structure. Phenol is a group formed by direct linking of hydroxyl group (-OH) and aromatic hydrocarbon ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/28
CPCG01N27/28
Inventor 袁勇蔡茜茜周丽华
Owner GUANGDONG UNIV OF TECH