Unlock instant, AI-driven research and patent intelligence for your innovation.

Simultaneous Determination of Free Amino Acids and Amadori Compounds in Tobacco by LC-MS/MS

A technology of free amino acids and compounds, applied in measuring devices, material separation, instruments, etc., can solve problems such as high-throughput analysis of unfavorable samples, narrow gradient range, damage, etc., and achieve good separation effect and good linear relationship

Inactive Publication Date: 2019-08-30
GUIZHOU TOBACCO SCI RES INST
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nonafluorovaleric acid will continue to accumulate residues in the chromatographic column, causing changes and damage to the properties of the chromatographic column. Long-term use will also pollute the mass spectrometer, and the use of ion-pairing reagents requires longer chromatographic column balance and elution time. Higher requirements for experimental conditions and stability of instruments and equipment, which is not conducive to long-term high-throughput analysis of samples
Cao Changchun et al. used the hydrophilic interaction chromatography (HILIC) method to simultaneously determine 2 kinds of amino acids and their corresponding Amadori compounds and other 5 substances. Limitations in the analysis of multiple target compounds in

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Simultaneous Determination of Free Amino Acids and Amadori Compounds in Tobacco by LC-MS/MS
  • Simultaneous Determination of Free Amino Acids and Amadori Compounds in Tobacco by LC-MS/MS
  • Simultaneous Determination of Free Amino Acids and Amadori Compounds in Tobacco by LC-MS/MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of working solution

[0033] Weigh 16 kinds of amino acid compound reference substances, and use ultrapure aqueous solution to prepare mixed amino acid reference substance stock solutions with a concentration of 5 μmol / mL for future use. Accurately weigh the appropriate amount of 6 kinds of Amadori compound standard substances, and prepare the mixed stock solution of 6 kinds of reference substances with ultrapure water, the concentration of which is 1000 μg / mL. Weigh an appropriate amount of 2-ABA and Val-d8 internal standards, use water as the diluent, and prepare primary internal standard mixed stock solutions with concentrations of 39mmol / mL and 16mmol / mL, respectively. Store in a refrigerator at 4°C.

[0034] Using water as the diluent, prepare a series of mixed standard solutions of 16 kinds of amino acids with concentrations of 0.01, 0.05, 0.10, 0.5, 1, 5, 10, 25, 50, 75, 125, and 250 μmol / L; prepare a Pro concentration of 500 μmol / L series of s...

Embodiment 2

[0044] Due to the strong polarity of free amino acids and Amadori compounds, according to the method of Example 1 of the present invention, the present invention compared the commonly used water, 0.1% formic acid, 0.1% acetic acid, 0.1% HCl, and 20% methanol water ( Both are volume ratios) and other polar extraction solvents to the extraction effect of amino acids and Amdori compounds. The results in Table 2 show that for most of the target compounds, the extraction efficiency of water is slightly higher than that of several other extraction solvents. When using 0.1% HCl as the extraction solvent, Arg, Tyr, Ile, Leu, Fru-Pro, Fru-Val The measured content is obviously reduced, only 30.4%-65.4% of the water extraction content, so the present invention selects water as the extraction solvent.

[0045] Table 2 The influence of different extraction solvents on the extraction results

[0046]

[0047]

Embodiment 3

[0049] According to the method of Example 1 of the present invention, water is used as the extraction solvent, and the extracts are respectively extracted for 10, 20, and 30 minutes, and the influence of the extraction time on the extraction results is compared. The results in Table 3 show that the extraction is complete when the extraction time is 10min, so the present invention preferably selects 10min as the optimum extraction time.

[0050] The influence of table 3 different extraction time on extraction result

[0051]

[0052]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for simultaneously measuring free amino acid and an Amadori compound in tobacco by virtue of LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry). A sample is extracted with a solvent, and is qualitatively and quantitatively determined by LC-MS / MS analysis, wherein a liquid chromatographic column is an Acclaim Explosive E2 analytical chromatographic column. According to the method, adoption of a tedious derivatization step and a complicated liquid chromatographic condition is avoided, and the method has the characteristics of simplicity for pretreatment, high sensitivity and high specificity, and is applied to quantitative analysis of a target compound in a high-throughput tobacco sample.

Description

technical field [0001] The invention belongs to the field of component analysis in tobacco, in particular to a method for simultaneously measuring free amino acids and Amadori compounds in tobacco by LC-MS / MS. Background technique [0002] Maillard reaction is a series of non-enzymatic browning reactions between carbonyl compounds and amino compounds. It occurs during the ripening and curing of tobacco leaves and has a significant impact on the quality and color of flue-cured tobacco. Amino acids and Amadori compounds (1-oxyl-1-deoxy-2-ketose) are important reaction substrates and primary products of the Maillard reaction, and the study of their content and their changing rules can be used to evaluate the quality of tobacco and strengthen the role of these compounds in tobacco production. It plays a guiding role in the monitoring of tobacco leaf preparation, alcoholization, cigarette processing, storage and other processes, as well as in optimizing cigarette formula design a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张婕耿召良杨慧朱文静向章敏葛永辉
Owner GUIZHOU TOBACCO SCI RES INST