A kind of method utilizing immobilized enzyme to prepare Jerusalem artichoke fructo-oligosaccharide
A technology of fructooligosaccharides and immobilized enzymes, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve the problem of low purity of fructooligosaccharides , to achieve the effect of continuous operation, lower production cost and high purity
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Embodiment 1
[0029] Embodiment 1: Preparation of inulin refined liquid.
[0030] Get 500g of fresh Jerusalem artichoke, wash, peel, grind and crush; add the crushed Jerusalem artichoke to the Tris-HCl buffer solution containing pectinase, the concentration of the Tris-HCl buffer solution is 1mol / L, and the pH is 6.8 , the amount of pectinase added is 1000U / L, stirred at 35 degrees Celsius for 3 hours, then heated the extract to 100 degrees Celsius, stirred for 5 hours; filtered the inulin extract to obtain a crude inulin extract;
[0031] Carry out decolorization, debittering, and desalination treatment to the inulin crude extract, pass the inulin crude extract through activated carbon, chitosan, cationic macroporous resin, and nanofiltration membrane successively, the cationic macroporous resin is H103, and the The nanofiltration membrane used is DL8040F to obtain the inulin refined solution.
Embodiment 2
[0033] The endo-inulinase used in the present invention is purchased from Novozymes, and the preparation method of chitosan / silica immobilized endo-inulinase is as follows:
[0034] (1a) Take 1-10g of mesoporous silica and 5-20g of chitosan in 100-150mL ethanol, react at 50-100°C for 10-24h, filter, wash with ethanol, and dry to obtain chitosan-modified silica carrier;
[0035] (2a) adding the chitosan-modified silica carrier obtained in step (1a) into a glutaraldehyde aqueous solution with a volume fraction of 1% to 4%, reacting for 2 to 10h, filtering, washing the filtrate with water, and then Add the filtrate to an aqueous phosphoric acid solution with an endo-inulinase concentration of 1-5 mg / L, react at 30-40°C for 1-10 hours, and filter with suction to obtain chitosan / silicon dioxide immobilized inulin Dicer.
Embodiment 3
[0036] Example 3: Effect of glutaraldehyde concentration on the enzyme activity of immobilized inulinase.
[0037] The preparation of the immobilized carrier and the immobilization method refer to Examples 1-3, the difference is that when the enzyme is immobilized, the volume concentration of glutaraldehyde is 0, 1%, 2%, 3%, 4%, 5%.
[0038] Table 1 Effect of glutaraldehyde concentration on the enzyme activity of immobilized inulinase
[0039]
[0040]
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