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Nano-copper/graphene-modified electrode ginsenoside Rg1 molecular imprinting sensor

A technology of ginsenoside and molecular imprinting, which is applied in the direction of material analysis, instruments, and scientific instruments through electromagnetic means, can solve the problems of high detection limit, difficult control of imprinted film thickness, slow electron transfer speed and response, and achieve high affinity. Harmony and selectivity, response enhancement effect

Inactive Publication Date: 2017-09-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high degree of cross-linking makes the electron transfer speed and response slow, and the lower limit of detection is high, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of nano-copper / graphene modification solution: In the reactor, add deionized water: 83 mL, copper acetate: 4 g, graphene oxide: 8 g, ultrasonically for 20 min at room temperature, disperse evenly, and then add ascorbic acid: 5g, heated to 60±2°C, stirred and reacted for 60min to obtain nano-copper / graphene modification solution;

[0025] (2) Preparation of nano-copper / graphene modified electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with double-distilled water, wash with ethanol, dry it, and drop it on the surface of the glassy carbon electrode Add 22 μL of nano-copper / graphene modification solution, place it under an infrared lamp, and evaporate the solvent to obtain a nano-copper / graphene modified electrode preparation;

[0026] (3) Preparation of ginsenoside Rg1 molecularly imprinted polymer: In the reactor, add methanol: 84 mL, divinyl styrene glycol dimethacrylic acid: 8 g,...

Embodiment 2

[0029] (1) Preparation of nano-copper / graphene modification solution: In the reactor, add deionized water: 86 mL, copper acetate: 2 g, graphene oxide: 6 g, ultrasonically for 20 min at room temperature, disperse evenly, and then add ascorbic acid: 6g, heated to 60±2°C, stirred and reacted for 60min to obtain nano-copper / graphene modification solution;

[0030](2) Preparation of nano-copper / graphene modified electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, wash with ethanol, dry it, and drop it on the surface of the glassy carbon electrode Add 23 μL of nano-copper / graphene modification solution, place it under an infrared lamp, and evaporate the solvent to obtain a nano-copper / graphene modified electrode preparation;

[0031] (3) Preparation of ginsenoside Rg1 molecularly imprinted polymer: In the reactor, add methanol: 78 mL, divinyl styrene glycol dimethacrylic acid: 10 g, ...

Embodiment 3

[0034] (1) Preparation of nano-copper / graphene modification solution: In the reactor, add deionized water: 80 mL, copper acetate: 5 g, graphene oxide: 12 g, ultrasonically for 20 min at room temperature, disperse evenly, and then add ascorbic acid: 3g, heated to 60±2°C, stirred and reacted for 60min to obtain nano-copper / graphene modification solution;

[0035] (2) Preparation of nano-copper / graphene modified electrode: Polish the glassy carbon electrode with 0.2 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, wash with ethanol, dry it, and drop it on the surface of the glassy carbon electrode Add 20 μL of nano-copper / graphene modification solution, place it under an infrared lamp, and evaporate the solvent to obtain a nano-copper / graphene modified electrode preparation;

[0036] (3) Preparation of ginsenoside Rg1 molecularly imprinted polymer: In the reactor, add methanol: 88 mL, divinyl styrene glycol dimethacrylic acid: 6 g,...

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Abstract

The invention discloses a preparation method of a nano-copper / graphene-modified electrode ginsenoside Rg1 molecular imprinting sensor. The method comprises the steps of firstly modifying a glassy carbon electrode by using copper acetate and graphene oxide to obtain a nano-copper / graphene-modified electrode; adding the following components in percentages by weight: 62-70% of methanol, 6-12% of diethylbenzene glycol dimethacrylate, 4-8% of itaconic acid, 7-16% of 1-allyl-3-ethyl imidazole bromide, 1.0-3.0% of azodiisobutyronitrile and 8-12% of ginsenoside Rg1 to a reactor, reacting in an anaerobic atmosphere, and removing a template molecule by using a mixed solution of methanol and acetic acid to obtain a ginsenoside Rg1 molecular imprinting polymer; and dispensing the ginsenoside Rg1 molecular imprinting polymer on a nano-copper / graphene-modified electrode to obtain the ginsenoside Rg1 molecular imprinting sensor. The sensor has high compatibility and selectivity, has the advantages of being high in sensitivity, good in specificity and fast in detection, and can be repeatedly used.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of a nano-copper / graphene modified electrode ginsenoside Rg1 molecularly imprinted sensor, which is used to detect medicines, food, and biological samples Ginsenoside Rg1 in. Background technique [0002] Ginsenoside is a sterol compound, triterpene saponin. It is mainly found in ginseng medicinal materials. Ginsenosides are considered to be the active ingredients in ginseng and thus have been the subject of research. Because ginsenosides affect multiple metabolic pathways, their efficacy is also complex, and the monomeric components of various ginsenosides are difficult to separate and detect. Ginseng contains a variety of active ingredients. Ginsenoside is the main active ingredient in ginseng. Ginsenoside monomers have been isolated and identified 46 species, of which...

Claims

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Application Information

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IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 李慧芝李冬梅杨秋苹
Owner UNIV OF JINAN
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