Gene microfluidic chip for detecting 26 animal-derived components at same time and application thereof

Abstract

The invention provides a gene microfluidic chip for detecting 26 animal-derived components at the same time and application thereof, and belongs to the technical field of animal food detection. 32 combinations each of which consists of a pairs of primers and a probe are fixed to the gene microfluidic chip, and the nucleotide sequences are shown as SEQ ID NO.1-96; fluorescence quantitative PCR detection is performed on a sample to be detected, and according to a Ct value, whether the sample to be detected contains any of the 26 animal-derived components is judged. By a detection method provided by the invention, rapid identification detection of the 26 animal-derived components can be completed within 3 hours; the detection method has the advantages of accurate detection, high sensitivity, strong specificity, simpleness, convenience and rapidness; the lowest detection limit is 1pg; requirements on large-batch and rapid identification detection of the 26 animal-derived components can be met; adulteration of meat products can be effectively resisted so as to enhance protection of the interests of consumers, so that the social benefits are relatively high.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a gene microfluidic chip for detecting 26 kinds of animal-derived components in food and its application. Background technique [0002] The identification of animal-derived ingredients in food is an important part of today's food safety testing. In 2013, the incident of beef mixed with horse meat caused a sensation in Europe. Maternity fakes are also common in the Chinese market. Duck meat is often used. Cheap meat such as chicken is sold as beef and mutton, and there have even been incidents of poisoning consumers by mixing rat meat poisoned by rat poison in meat products. These incidents have seriously disrupted the order of the food market and violated the interests of consumers. In severe cases, they may cause ethnic problems and disrupt social harmony. [0003] With the continuous development of the market, the technical means of meat adulteration are also constantly...

AI Technical Summary

Problems solved by technology

At present, there are reports of 16 kinds of animal-derived components using aldehyde-based glass slides as solid-phase carriers to make gene chips (Zong Hui, 2011 application number: 201110110348.2). This method requires PCR reaction first, and then the probes on the chip Perform hybridization, and finally perform fluorescence scanning and data processing on the hybridized chip, with many steps and cumbersome methods

And during the reaction, all the probes are placed in the same liquid environment for the reaction, and the probes are susceptible to environmental interference, thereby reducing sensitivity and specificity

When multiple probes are integrated on the chip, their specificity is often lower than that of a single probe

In addition, this kind of chip is easy to break, which is not conducive to long-term storage

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