Chondroitin sulfate A nano-selenium micelle particle, and preparation method and application thereof

A chondroitin sulfate and nanomicelle technology, which can be used in sulfur/selenium/tellurium active ingredients, bone diseases, pharmaceutical formulations, etc., can solve problems such as low biocompatibility, and achieve low cytotoxicity, good dispersion, Toxicity reduction effect

Active Publication Date: 2017-10-20
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is reported in the prior art that artificially synthesized chemical product chondroitin sulfate, although the cost of artificially synthesized chondroitin sulfate is reduced, but due to the problem of oversulfation, the generated chondroitin sulfate product cannot be metabolized in the body due to the lack of corresponding metabolic enzymes. May have adverse effects on the body, such as the body's immune response, allergic reaction, gastrointestinal discomfort and other symptoms, compared with bioactive chondroitin sulfate, the biocompatibility is lower

Method used

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  • Chondroitin sulfate A nano-selenium micelle particle, and preparation method and application thereof
  • Chondroitin sulfate A nano-selenium micelle particle, and preparation method and application thereof
  • Chondroitin sulfate A nano-selenium micelle particle, and preparation method and application thereof

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Effect test

Embodiment 1

[0034] 1) Weigh 0.495g chondroitin sulfate A and dissolve it in 3ml distilled water, stir well to obtain an aqueous solution of chondroitin sulfate A; 0.011g sodium selenite (Na 2 SeO 3 ) was dissolved in 1ml of water to obtain an aqueous solution of sodium selenite; under ultrasonic conditions of 60 Hz and 30°C, the aqueous solution of selenium compound was added to the aqueous solution of chondroitin sulfate A to obtain a mixed solution;

[0035] 2) Add 0.033g of L-ascorbic acid and 2ml of water to fully stir until dissolved to obtain an aqueous solution of L-ascorbic acid, and then add the aqueous solution of L-ascorbic acid dropwise to the mixed solution prepared in step 1) under stirring, 60Hz, 30 ℃, sonicate for 1 hour, then put the obtained solution into a dialysis bag, put it into a deionized aqueous solution for dialysis overnight, replace the deionized water on the second day and continue dialysis, and replace the deionized water every 4 hours until the dialysis bag ...

Embodiment 2

[0038] 1) Take by weighing 0.490g chondroitin sulfate A and dissolve in 3ml distilled water, fully stir, and obtain the chondroitin sulfate A aqueous solution; 0.022g sodium selenite (Na 2 SeO 3 ) was dissolved in 1ml of water to obtain an aqueous solution of sodium selenite; under ultrasonic conditions of 60 Hz and 30°C, the aqueous solution of selenium compound was added to the aqueous solution of chondroitin sulfate A to obtain a mixed solution;

[0039] 2) Add 0.066g of L-ascorbic acid and 2ml of water to fully stir until dissolved to obtain an aqueous solution of L-ascorbic acid, and then add the aqueous solution of L-ascorbic acid dropwise to the mixed solution prepared in step 1) under stirring, 60Hz, 30 ℃, sonicate for 1 hour, then put the obtained solution into a dialysis bag, put it into a deionized aqueous solution for dialysis overnight, replace the deionized water on the second day and continue dialysis, and replace the deionized water every 4 hours until the dial...

Embodiment 3

[0042] 1) Take by weighing 0.475g chondroitin sulfate A and dissolve it in 3ml distilled water, fully stir to obtain the chondroitin sulfate A aqueous solution; 0.055g sodium selenite (Na 2 SeO 3 ) was dissolved in 1ml of water to obtain an aqueous solution of sodium selenite; under ultrasonic conditions of 60 Hz and 30°C, the aqueous solution of selenium compound was added to the aqueous solution of chondroitin sulfate A to obtain a mixed solution;

[0043] 2) Add 0.165g of L-ascorbic acid and 2ml of water to fully stir until dissolved to obtain an aqueous solution of L-ascorbic acid, and then add the aqueous solution of L-ascorbic acid dropwise to the mixed solution prepared in step 1) under stirring, 60Hz, 30 ℃, sonicate for 1 hour, then put the obtained solution into a dialysis bag, put it into a deionized aqueous solution for dialysis overnight, replace the deionized water on the second day and continue dialysis, and replace the deionized water every 4 hours until the dia...

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Abstract

The invention discloses a chondroitin sulfate A nano-selenium micelle particle, and a preparation method and application thereof, which belong to the technical field of medical nanometer materials. The preparation method comprises the steps of adopting chondroitin sulfate A as a carrier, combining nano-selenium on the chondroitin sulfate A through physical adsorption so as to form the nano-selenium micelle particle, wherein the mass fraction of selenium is 0.1 percent to 25 percent. The chondroitin sulfate A nano-selenium micelle provided by the invention combines dual pharmacological activity of the chondroitin sulfate A and the nano-selenium, so that the toxicity of inorganic selenium is reduced, and the bioavailability is improved; meanwhile, by combining the biological activity of the chondroitin sulfate A, the chondroitin sulfate A nano-selenium micelle particle can be better developed to be a novel nanomicelle preparation for preventing and improving osteoarthrosis.

Description

technical field [0001] The invention belongs to the technical field of medical nanomaterials, and relates to a chondroitin sulfate A nano-selenium micelle particle and a preparation method and application thereof. Background technique [0002] Selenium is an essential trace element for the human body. Sodium selenite preparations can be used clinically in the prevention and treatment of diseases such as hypertension, cancer, myocarditis, coronary heart disease, Keshan disease and Kaschin-Beck disease. However, selenium and its compounds are highly toxic, and the maximum safe daily dose of selenium is 400 μg. Excessive selenium can cause poisoning. It has been reported that the toxicity of organic nano-selenium is less than that of inorganic selenium, and its biological activity is higher than that of inorganic selenium, thus overcoming the weakness that the effective dose of inorganic selenium-containing substances is not much different from the toxic dose. Therefore, with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K9/19A61K33/04A61K47/22A61P19/02A61P19/08A61P19/04A61K31/737
CPCA61K9/1075A61K9/19A61K31/737A61K33/04A61K47/22A61K2300/00
Inventor 韩晶郭雄
Owner XI AN JIAOTONG UNIV
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