A kind of ganoderma lucidum liquid strain culture medium and ganoderma lucidum liquid strain cultivation method

A technology of liquid strains and cultivation methods, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of weak vitality, slow growth of Ganoderma lucidum, inconsistent bacterial age, etc. , The effect of strong disease resistance and stable quality

Active Publication Date: 2020-08-11
江阴市长泾国民育种场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention needs to provide a liquid culture medium of ganoderma lucidum and a method for cultivating ganoderma liquid strains, so as to solve the problem that ganoderma lucidum produced and cultivated by solid strains has slow germination and inconsistent bacterial age, weak disease resistance, weak vitality, and is easily affected by miscellaneous bacteria. Infection and other technical issues

Method used

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  • A kind of ganoderma lucidum liquid strain culture medium and ganoderma lucidum liquid strain cultivation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Make 1000ml liquid culture medium: peptone 6g, glucose 15g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 0.5g, potato starch 1g, corn tapioca starch 3g, vitamin B 1 1 tablet (10mg), agar 1g, purified water 1000ml.

[0021] Vial liquid culture method:

[0022] Prepare a 500ml-capacity triangular flask, fill the triangular flask with 200ml / bottle of liquid culture medium, sterilize the triangular flask in a high-pressure steam cooker, and sterilize at 120°C-126°C for 30 minutes, then take it out to cool, and take it out Ganoderma lucidum slant test tube parent species, pick 1 or 2 pieces the size of broad beans into the triangular bottle, let stand for 24 hours. After 24 hours, put the triangular flask into a rotary shaker and cultivate on the shaker. The characteristics of the shaker will affect the mass transfer effect of oxygen, which will affect the growth of mycelium. Therefore, the rotation speed should be controlled at 120-130r / min , the most suitable t...

Embodiment 2

[0024] Make 60 liters of liquid culture medium: peptone 250g, glucose 1000g, magnesium sulfate 40g, potassium dihydrogen phosphate 20g, potato starch 100g, corn tapioca starch 200g, vitamin B 1 60 tablets, 100g agar, 1 scoop (5g) of antifoaming agent, 60L purified water.

[0025] Access to the cultivation method of the fermenter:

[0026] 1. Prepare a 500ml Erlenmeyer flask, fill the Erlenmeyer flask with 200ml / bottle of liquid culture medium, sterilize the Erlenmeyer flask in a high-pressure steam cooker, sterilize at 120°C-126°C for 30 minutes, then take it out and cool it , Take a ganoderma ganoderma slant test tube parent plant, pick 1 or 2 pieces the size of broad beans and put them in a triangular bottle, and let them stand for 24 hours. After 24 hours, put the triangular flask into a rotary shaker and cultivate on the shaker. The characteristics of the shaker will affect the mass transfer effect of oxygen, which will affect the growth of mycelium. Therefore, the rotat...

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Abstract

The invention discloses a ganoderma lucidum liquid spawn medium formula which comprises 6g of peptone, 15g of glucose, 0.5g of magnesium sulfate, 0.5g of monopotassium phosphate, 1g of potato starch, 3g of corn, 3g of cassava starch, 10mg of vitamin B1, 1g of agar and 1000ml of purified water. The invention further discloses a ganoderma lucidum liquid spawn culture method. The culture method comprises the following steps: by taking the ganoderma lucidum liquid spawn medium formula as a basis, performing high-temperature sterilization, inoculating into stock culture, and culturing in a shake table, thereby obtaining the ganoderma lucidum liquid spawn. The ganoderma lucidum liquid spawn medium formula and the ganoderma lucidum liquid spawn disclosed by the invention have the advantages that the culture steps are reduced, the fermentation period is shortened, the growth period is shortened, the pollution rate is low, the cost is low, the yield is low, and the culture method is applicable to large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a ganoderma liquid strain culture medium and a ganoderma liquid strain cultivation method. Background technique [0002] At present, the cultivation method of Ganoderma lucidum is mainly solid culture, which is a traditional cultivation method. The quality and output of Ganoderma lucidum produced by traditional cultivation methods have been difficult to meet the needs of domestic and foreign markets. The Ganoderma lucidum produced and cultivated by solid strains has the disadvantages of slow germination, inconsistent bacterial age, weak disease resistance, weak vitality, and easy to be infected by miscellaneous bacteria. Contents of the invention [0003] The present invention needs to provide a liquid culture medium of ganoderma lucidum and a method for cultivating ganoderma liquid strains, so as to solve the problem that ganoderma lucidum produced and cultiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 张国民
Owner 江阴市长泾国民育种场
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