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A current-type electrochemical sensor and its preparation method and application

A sensor and electrochemical technology, applied in the field of amperometric electrochemical sensor and its preparation, can solve the problems of small detection range, unfavorable sensitivity, and low sensitivity

Inactive Publication Date: 2019-05-07
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conductivity of antibodies and blocking agents is not good, which is not conducive to the improvement of sensitivity; for non-standard electrochemical sensors, the change of current is only because the antigen is immobilized on the surface of the electrode, which has problems such as low sensitivity and small detection range

Method used

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  • A current-type electrochemical sensor and its preparation method and application
  • A current-type electrochemical sensor and its preparation method and application
  • A current-type electrochemical sensor and its preparation method and application

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preparation example Construction

[0023] The calcium carbonate nanoparticle probe prepared according to the present invention has poor conductivity and carries CA199 antibody 2 and glucose oxidase, so it can specifically recognize target analyte CA199 and catalyze glucose to produce hydrogen peroxide. When the probe is attached to the substrate, the resistance increases, reducing the electrochemical response current. The substrate is modified with heme and contains gold polymethylene blue as an electrical signal substance, and heme can catalyze the degradation of gold polymethylene blue in the presence of hydrogen peroxide.

[0024] The signal substance of the ordinary sandwich type electrochemical immunosensor is on the probe, but the probe must be modified with antibodies and blocking agents (such as bovine serum albumin), and these proteins have poor conductivity, which is not conducive to the increase of the response current.

[0025] According to the sensor of the present invention, the signal substance g...

preparation Embodiment 1

[0030] 1) Build the sensor

[0031] The glassy carbon electrode was ground with 0.05 micron alumina powder, washed in 1:1 nitric acid, ethanol and secondary water, and finally dried with nitrogen. Then 8 μL of gold polymethylene blue solution at a concentration of 2 mg / mL, 4 μL of gold particle-modified reduced graphene oxide solution at a concentration of 2 mg / mL, and 4 μL of platinum particle-modified sodium alginate solution at a concentration of 2 mg / mL were mixed, and the mixed droplets on the electrode surface. After drying in an oven at 37°C, the electrode was cooled in a refrigerator at 4°C, and then cooled in a concentration of 20nM CaCl 2 Soak in the solution for 10min. After washing with secondary water, 80 μL of CA199 antibody 1 (Ab1) with a concentration of 200 μg / mL was directly dropped on the surface of the electrode and stored in a refrigerator at 4 °C overnight, followed by bovine serum albumin (BSA) solution (mass percentage concentration of 1 %) to block ...

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Abstract

The invention discloses a current type electrochemical sensor, and a production method and an application thereof. The current type electrochemical sensor degrades signal substances in order to effectively improve the sensitivity of the sensor. The above sensor system comprises a sensor and a probe, a mixture comprising gold polymethylene blue or thionine, a reduced graphene oxide-hemin-gold particle and platinum particle modified sodium alginate or polyacrylic acid is crosslinked and immobilized on the surface of an electrode through Ca<2+> to form a substrate, a CA199 antibody 1 (Ab1) is adsorbed and immobilized on the substrate, and remaining active sites are blocked by bovine serum albumin to construct the current electrochemical sensor.

Description

technical field [0001] The invention relates to the technical field of biological immune detection, in particular to an amperometric electrochemical sensor and its preparation method and application. The amperometric electrochemical sensor effectively improves the sensitivity of the sensor by degrading signal substances. Background technique [0002] Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat. About 90% of them are ductal adenocarcinomas originating from the ductal epithelium. Its morbidity and mortality have increased significantly in recent years. The 5-year survival rate is <1%, and it is one of the malignant tumors with the worst prognosis. The early diagnosis rate of pancreatic cancer is not high, the surgical mortality rate is high, and the cure rate is very low. The incidence of this disease is higher in men than in women, with a male to female ratio of 1.5 to 2:1. Male patients are far more common...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N33/574
CPCG01N27/3277G01N27/3278G01N33/57438
Inventor 马占芳李卫祥
Owner CAPITAL NORMAL UNIVERSITY