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A kind of silk protein bioscaffold and its preparation method and application

A biological scaffold and silk protein technology, applied in the field of biomedical materials, can solve the problems of declining economic value of silk protein, high production cost, destruction of silk protein, etc., and achieve good biocompatibility and cell adhesion, low cost, The effect of promoting the exchange of nutrients

Active Publication Date: 2019-10-15
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, silkworm chrysalis has become a delicacy on people's dining tables. We know that silkworms pupate in silkworm cocoons, that is, silkworm chrysalis are wrapped in silkworm cocoons. At present, silkworm chrysalis production mainly adopts manual cutting to take out silkworm chrysalis from silk cocoons, which is laborious. Time-consuming, high production cost, and the silk protein is also destroyed in the process of cutting cocoons and taking pupae, which can no longer be used for silk reeling, and the economic value of silk protein has dropped significantly

Method used

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  • A kind of silk protein bioscaffold and its preparation method and application
  • A kind of silk protein bioscaffold and its preparation method and application
  • A kind of silk protein bioscaffold and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 prepares silkworm silk protein bioscaffold

[0031] (1) Select silkworm species

[0032] A wild-type silkworm variety (preserved by the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences and provided commercially) is selected, and the silk proteins produced by this type of silkworm variety include sericin and silk fibroin.

[0033] (2) Preparation of silkworm spinning platform

[0034] Use dry wood or cardboard with a thickness of 2 cm to make a cylinder (base) with a diameter and a height of 50 cm. Place the pedestal on a flat table, and cover its upward side with 4 layers of high-temperature sterilized newsprint with a thickness of 0.5 mm. Then cover a layer of cotton gauze (10 mesh) on the paper, and fix the edges of the cotton gauze.

[0035] (3) Breeding silkworm varieties

[0036] The wild-type silkworm species (Jingsong A or Baiyu) were selected and fed with fresh mulberry leaves under standard conditions (1-2 ages at ...

Embodiment 2

[0048] Example 2 Bombyx mori silk protein bioscaffold used as cell carrier

[0049] (1) Viability detection of mouse skin fibroblast (NIH3T3) silk protein bioscaffold

[0050] (1) First cut the fibroin bioscaffold into pieces, then wash it three times with sterilized PBS, then soak it with 75% ethanol for 1 hour, and finally wash it once with sterilized PBS, and keep it at room temperature for later use.

[0051] (2) The cells collected from the cell culture flask are suspended, blown off, and planted in a 96-well cell culture plate. After the cells are attached to the wall, the silk protein bioscaffold pretreated in step (1) is transferred to the cell containing the cell. In the culture plate, a piece of silk protein bio-scaffold is placed in each well. Cell culture wells without fibroin bioscaffolds were used as controls. The medium used for cell culture was DMEM high-glucose medium, and the cells were placed in a cell culture incubator (37°C, CO 2 The concentration is 5%...

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Abstract

The invention discloses a fibroin biological scaffold as well as a preparation method and an application thereof. The spinning physiological characteristics of silkworms are changed through a silkworm breeding technology by adopting a wild silkworm variety to obtain the fibroin biological scaffold for wound healing. The biological scaffold is capable of keeping the natural structure and the activity of fibroin and has the original ecological characteristics of the fibroin. The fibroin biological scaffold has a porous structure and good air permeability and has very high mechanical strength and high biocompatibility. The fibroin biological scaffold has good biocompatibility and cell adhesiveness, is capable of supporting cell survival, proliferation and migration for a long period of time. The fibroin biological scaffold can be applied to an extracellular matrix for supporting cell growth and promoting nutrient exchange, can be used as a medical filling material and can be applied to repairing of multiple tissue damages and disease treatment.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a silk protein, in particular to a silk protein biological scaffold and a preparation method and application thereof. Background technique [0002] Silk protein (including silk fibroin and sericin) is a natural animal protein secreted by silkworms. It contains 18 kinds of amino acids. It not only has degradable, antibacterial, anticancer, antioxidative, anticoagulant, hydrophilic, moisturizing, It has low immunogenicity and biological activities such as promoting cell adhesion and proliferation, and is rich in active groups such as amino, hydroxyl, and carboxyl groups that are conducive to modification or transformation. Nowadays, silk protein is widely used in the field of medicine as an important tissue engineering material . [0003] In recent years, people have developed different types of tissue engineering materials based on silk protein, such as silk protein hydrogels, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/56A61L27/58A01K67/04C12N5/00
Inventor 张国政张业顺方瑷吴堂凤梁培生李思儒何小柏夏定国布塔库孜·木拉提别克
Owner JIANGSU UNIV OF SCI & TECH