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A kind of sericin wound dressing and its preparation method and application

A technology of sericin and wound dressing, which is applied in the fields of tumor/cancer cells, bandages, animal husbandry, etc. It can solve the problems of natural structure damage of sericin, change of natural biological activity, complex preparation process, etc., and achieve good biocompatibility Sexuality and cell adhesion, high biocompatibility, good air permeability

Active Publication Date: 2019-10-15
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, based on the research and development of sericin materials, the sericin used is processed through separation and purification and a series of subsequent physical and chemical treatments. In these processes, it is inevitable to cause the natural structure of sericin Disruption (molecular weight and native conformation) and alteration of native biological activity
At the same time, the preparation process of sericin wound dressing is relatively complicated, the cost is high and the mechanical properties are not good.

Method used

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  • A kind of sericin wound dressing and its preparation method and application
  • A kind of sericin wound dressing and its preparation method and application
  • A kind of sericin wound dressing and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 prepares silkworm sericin wound dressing

[0035] (1) Select silkworm species

[0036] A mutant silkworm variety with a lack of silk fibroin (preserved and commercially provided by the Institute of Sericulture, Chinese Academy of Agricultural Sciences) is selected, and the silk protein produced by this type of silkworm variety only contains sericin and does not contain silk fibroin.

[0037] (2) Preparation of silkworm spinning platform

[0038] Select 6 pieces of cardboard with a thickness of 1cm and a length and width of 50cm to make a surface area of ​​1.5m 2 cube cardboard box. Place the carton on a flat table, and cover the upward side with 3 layers of high-temperature sterilized newsprint with a thickness of 0.4 mm. Then cover a layer of cotton gauze (10 mesh) on the paper, and tighten the four sides of the cotton gauze.

[0039] (3) Breeding silkworm varieties

[0040] Silkworm silk fibroin-deficient mutant varieties (185Nds or 140Nds) were sele...

Embodiment 2

[0054] Example 2 Bombyx mori sericin wound dressing is used as a cell carrier

[0055] (1) Sericin wound dressing cell viability assay of mouse skin fibroblasts (NIH3T3) and human skin keratinocytes (Hacat)

[0056] (1) First cut the sericin wound dressing into pieces, then wash it three times with sterilized PBS, then soak it with 75% ethanol for 1 hour, and finally wash it once with sterilized PBS, and keep it at room temperature for later use.

[0057] (2) The cells collected from the cell culture flask were suspended, blown off, and planted in a 96-well cell culture plate. After the cells adhered to the wall, the sericin wound dressing pretreated in step (1) was transferred to the cell culture plate containing the cells. A piece of sericin wound dressing was placed in each well of the culture plate. Cell culture wells without sericin wound dressings were used as controls. The medium used for cell culture was DMEM high-glucose medium, and the cells were placed in a cell c...

Embodiment 3

[0068] Example 3 Bombyx mori sericin wound dressing is used for mouse skin injury repair

[0069] (1) First cut the sericin wound dressing into a circular piece with a diameter of 1 cm, then wash it three times with sterilized PBS, then soak it with 75% ethanol for 1 hour, and finally wash it once with sterilized PBS, and dry it Then keep it at room temperature for later use.

[0070] (2) A mouse model of scald was prepared, and the selected animals were 6-week-old Kunming rats. The first choice is to anesthetize and depilate the mouse; then use a metal rod (a cylinder with a diameter of 1 cm) at a temperature of 100° C. to stick to the back of the mouse for 5 seconds. Then the scalded skin was cut off, the sericin wound dressing pretreated in step (1) was placed on the wound surface, and the mice not treated with the wound dressing were used as controls.

[0071] Experimental results such as Figure 15 As shown, after 2 days, the wound area of ​​the mice in the sericin wou...

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Abstract

The invention discloses a sericin wound dressing, and a preparation method and application thereof. The sericin wound dressing capable of being used for wound repair can be obtained by using fibroin deficient mutant silkworm varieties, combining a silkworm breeding technology and changing the spinning physiological habits and characteristics of silkworms. The wound dressing has natural structures and activity of sericin; the original ecology characteristic of the sericin is maintained. The sericin wound dressing is of a porous structure and has good air permeability; good mechanical strength is realized; the surgical suture is convenient; high biocompatibility is realized; the skin wound healing can be effectively promoted; the sericin wound dressing can be used for skin tissue wound repair. The sericin wound dressing has good biocompatibility and cell adhesivity, and can support the survival, propagation and migration of cells for a long time. The dressing is also a three-dimensional porous sericin biological scaffold, can be used for a extracellular matrix for supporting the cell growth and promoting the nutrient substance exchange, and can be applied to various tissue wound repair and disease treatment.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to sericin, in particular to a sericin wound dressing and a preparation method and application thereof. Background technique [0002] Sericin is a natural animal protein secreted by the silkworm, containing 18 kinds of amino acids, not only has degradable, antibacterial, anticancer, antioxidative, anticoagulant, hydrophilic, moisturizing, non-immunogenic and promotes cell adhesion And proliferation and other biological activities, and rich in amino, hydroxyl, carboxyl and other active groups that are conducive to modification or transformation, sericin has become a new type of tissue engineering material that has attracted much attention. [0003] In recent years, different types of tissue engineering materials have been developed based on sericin, such as hydrogels, sericin membranes, sericin sponge-like materials, sericin nanofibers and nanoparticles, etc. However, so far, based...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/40A61L15/32A61L15/42A01K67/04C12N5/09
Inventor 张业顺张国政方瑷吴堂凤陆莹霞李木旺梁培生何小柏李聪
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD