A method for removing diester-type alkaloids in Clematis aureus extract

A diester-type alkaloid and extract technology, which can be applied in drug combinations, blood diseases, and pharmaceutical formulas, can solve the problems of limited health care and treatment functions, affecting efficacy and taste, and high content of A. Easy to operate, less toxic and side effects, and low operating costs

Active Publication Date: 2020-07-03
JIAYING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is the high toxicity of diester alkaloids such as aconitine, and the high content in clematis, which greatly limits the health care and therapeutic functions of clematis.
[0006] Since the extract of Auranthus clematis is used for the health care and treatment of living bodies, it is not possible to add some toxic and side effect chemical substances to remove diester alkaloids such as aconitine, even if some substances with little toxic and side effects can be added to a certain extent. It will affect the efficacy and taste. Moreover, various substances in the extract of Aurantia clematis are dissolved in one body, and their separation and purification are very difficult.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The extract in the present embodiment is the extract of Clematis clematis prepared with water as the solvent. After testing, the content of total alkaloids (calculated as ephedrine hydrochloride, the same below) in the solution is 34.36 mg / L, and the diester type The content of alkaloids (calculated as aconitine) is 17.70 mg / L.

[0021] The implementation process is as follows: In a 2-liter round-bottomed flask, add 1 liter of Clematis clematis extract and 100 milliliters of an aqueous solution containing 20 mg of glycyrrhizic acid, heat to boiling, keep warm and reflux for 1.5 hours, cool to room temperature, and filter. The filter residue is recovered and used for other purposes, and the filtrate is the extract of Auranthus clematis after removing diester alkaloids.

[0022] After testing, the total alkaloid content in the extract of Auranthus clematis treated with glycyrrhizic acid was 15.63 mg / L, and the monoester alkaloid content (calculated as aconitine, the same ...

Embodiment 2

[0025] The extract in this example is the Aurantia clematis extract prepared with ethanol as a solvent. The total alkaloid content in the solution is 53.68 mg / L, and the diester alkaloid content is 33.17 mg / L.

[0026] The implementation process is as follows: In a 2-liter round-bottomed flask, add 1 liter of Clematis clematis extract and 100 milliliters of ethanol solution containing 34 mg of glycyrrhetinic acid respectively, heat to boiling, keep warm and reflux for 1.5 hours, cool to room temperature, and filter . The filter residue is recovered and used for other purposes, and the filtrate is the extract of Auranthus clematis after removing diester alkaloids.

[0027] After testing, the total alkaloid content in the Aureus aureus extract treated with glycyrrhetinic acid was 24.61 mg / L, the monoester alkaloid content was 6.81 mg / L, and no diester aconitine was detected; recovered Diester-type aconitine and other alkaloids were detected in the filter residue.

[0028] In t...

Embodiment 3

[0030] The extract in the present embodiment is the Auranthus clematis extract prepared by using 50% ethanol (ethanol-water volume ratio, the same below) as the solvent. After testing, the total alkaloid content in the solution is 46.45 mg / liter, double The content of ester alkaloids is 24.27 mg / L.

[0031] The implementation process is as follows: in 2 liters of round-bottomed flasks, add 1 liter of Clematis clematis extract, 100 milliliters of 50% ethanol solution containing 25 mg of oleanolic acid respectively, heat to boiling, heat preservation and reflux reaction for 1.5 hours, and cool to room temperature, filter. The filter residue is recovered and used for other purposes, and the filtrate is the extract of Auranthus clematis after removing diester alkaloids.

[0032] After testing, the total alkaloid content in the extract of Auranthus clematis treated with oleanolic acid was 21.65 mg / L, the monoester type alkaloid content was 4.69 mg / L, and no diester type aconitine ...

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PUM

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Abstract

The invention discloses a method for removing diester-type alkaloids from an anoectochilus roxburghii extracting solution. The method comprises the steps of calculating the content of the diester-type alkaloids in the anoectochilus roxburghii extracting solution according to the content of aconitine, adding triterpenes organic acid, which is 1 to 1.5 times the mass of the aconitine, into the anoectochilus roxburghii extracting solution, heating the solution, performing refluxing reaction for 1.5 to 2 hours, naturally cooling a reaction product to room temperature, and filtering the reaction product, thus obtaining filtrate which is an anoectochilus roxburghii extracting solution without the diester-type alkaloids. According to the method, the diester-type alkaloids in the anoectochilus roxburghii extracting solution is effectively removed; the method is easy and convenient to operate; used equipment is simple; the operation cost is low.

Description

technical field [0001] The invention relates to a method for removing diester-type alkaloids in the extract of Auranthus clematis. Background technique [0002] Anoectochilus roxburghii (Wall.) Lindl.], also known as Anoectochilus roxburghii (Wall.) Lindl.], also known as Anoectochilus roxburghii (Wall.) Lindl.], also known as Anoectochilus roxburghii (Wall.) Lindl. Orchid orchid is a rare medicinal plant, a perennial herb, mainly distributed in subtropical and tropical regions such as Guangdong, Fujian, and Taiwan. [0003] Aurantia clematis is known as "King of Medicine" and "God Grass" among the people, and has a wide range of medicinal value. "Dictionary of Chinese Medicine", "Cihai of Chinese Medicine", "Supplements to Materia Medica", "Chinese Materia Medica", "Chinese Economic Flora" and other famous classics have records of Aurantia clematis. Its nature and taste are sweet and flat, and it has the effects of clearing heat and cooling blood, expelling wind and dampn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/898A61P3/10A61P3/06A61P9/12A61P1/16A61P29/00A61P25/20A61P7/10A61P39/06A61P35/00
CPCA61K36/898
Inventor 吕鑑泉
Owner JIAYING UNIV
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