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Leccinum extremiorientale antioxidant polysaccharides and extracting and separating process thereof

A technology for oxidizing polysaccharides and boletus, which is applied in the fields of anti-virus, food science, drug combination, etc., to achieve the effect of product safety

Active Publication Date: 2017-11-10
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are relevant reports on the research on the chemical components of Boletus fruticosa, the polysaccharide research of this species has not been reported yet. The present invention uses the fruiting body of Boletus fruticosa as material to extract the antioxidant polysaccharides. Conditions and processes are optimized

Method used

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  • Leccinum extremiorientale antioxidant polysaccharides and extracting and separating process thereof
  • Leccinum extremiorientale antioxidant polysaccharides and extracting and separating process thereof
  • Leccinum extremiorientale antioxidant polysaccharides and extracting and separating process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Optimization of Polysaccharide Extraction and Separation Conditions of Boletus fareastica Boletus

[0029]1. Pretreatment of raw materials: Weigh 2g of Boletus farrescens fruiting bodies, dry, crush, pass through a 100-mesh sieve, add 4 times the volume of 80% ethanol (w / v=1:4), and place in a water bath at 60°C Bath in medium water for 2 hours, centrifuge at 3500 rpm for 5 minutes, take the precipitate, and evaporate to dryness in a water bath at 60°C to obtain defatted boletus fruiting body powder, which is stored for future use.

[0030] After extracting the defatted boletus fruiting body powder with hot water, centrifuge at 3500rpm for 10 minutes to obtain the polysaccharide supernatant, concentrate it to 1 / 3 of the original volume with a rotary evaporator, add 4 times the volume of 80% ethanol at 35-40°C, and heat at 4°C Precipitate with alcohol for 24 hours, centrifuge at 3500rpm for 10-15min, take the polysaccharide supernatant, mix the supernatant with...

Embodiment 2

[0051] Example 2 Detection of antioxidant activity of boletus polysaccharide LEP-2

[0052] 1. Determination of scavenging·OH activity: prepare 9mmol / L FeSO 4 (Preparation now), 9mmol / L salicylic acid-ethanol solution, 20mmol / L H 2 o 2 (Liu, 2016). Add each reaction solution sequentially according to Table 4, react at 37°C for 1 hour, and measure OD 510nm value, without sample, denoted as A 0 ; There is a sample, denoted as A 1 ;no H 2 o 2 , denoted as A 2 ;V C as a positive control.

[0053] OH scavenging rate (%) = [1-(A 1 -A 2 ) / A 0 ]×100

[0054] Table 4 Determination of hydroxyl radical scavenging ability of polysaccharides

[0055] components

[0056] 2. Clear O 2 - Determination of activity: prepare 50 mM Tris-HCl (pH=8.2), 0.05-1 mg / mL polysaccharide solution, and 25 mM pyrogallol solution (Liu, 2015). Add the reaction solution according to Table 5, and react at 25°C for 5 minutes. The reaction was stopped with 1 mL of 8 mM HCl. Measure OD ...

Embodiment 3

[0065] Example 3 Structural Analysis of the Antioxidant Polysaccharide LEP-2 of Boletus fareasterensis

[0066] 1. Analysis of monosaccharide components

[0067] (1) Pretreatment for determination of monosaccharide composition: Weigh 8.2mg of fibrous LEP-2 and place it in a high temperature resistant glass tube, add 2mL of 2mol / L trifluoroacetic acid (TFA), and blow N 2 , After removing the air in the glass tube, seal it quickly. Hydrolyze at 121°C (oil bath) for 2h. The sample solution was diluted 50 times and injected.

[0068] (2) Chromatographic conditions: use high performance liquid chromatography to analyze the monosaccharide composition of LEP-2, and measure the OD 250nm value; keep the temperature of the column at room temperature; and the elution flow rate is 0.5mL / min; the mobile phase is as follows:

[0069] time (min)

water(%)

250mM NaOH(%)

0

98

2.0

30

98

2.0

[0070] (3) Preparation of monosaccharide standard sub...

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Abstract

The invention provides an extracting and separating process of leccinum extremiorientale antioxidant polysaccharides. The method includes the following steps of firstly, conducting degreasing treatment on leccinum extremiorientale sporocarp to obtain leccinum extremiorientale sporocarp powder; secondly, extracting the degreased leccinum extremiorientale sporocarp powder through hot water, sequentially conducting concentration, alcohol precipitation, deproteinization, depigmentation and dialysis on aqueous extracts in sequence, and then conducting drying to obtain crude polysaccharide products; thirdly, conducting ion-exchange column chromatography and sieve chromatography to purify the crude polysaccharide products, collecting eluant, and conducting freezing and drying to obtain the leccinum extremiorientale antioxidant polysaccharides. Through in-vitro free radical scavenging capacity experiments, the result shows that polysaccharide components with molecular weight of 342375 Da have a remarkable anti-oxidation effect. The developed products are safe, and the leccinum extremiorientale antioxidant polysaccharides can be widely used as food additives.

Description

technical field [0001] The invention relates to an antioxidant substance in edible fungi and an extraction process thereof, in particular to an anti-oxidation polysaccharide of Boletus verrucosus and its extraction and separation process. Background technique [0002] Free radicals are an intermediate metabolite produced by organisms in the process of metabolism. Like other reactive oxygen species (ROS), there is a dynamic balance, but when there is an excess in the body, it will cause oxidative damage and cause some chronic diseases (Liu et al., 2016). In addition, free radicals can also react with oxidative damage to important biological macromolecules in the body, such as DNA, protein, carbohydrates, etc., which will reduce biological metabolic reactions and cause related diseases. The most effective way to eliminate ROS is to use antioxidants. But synthetic antioxidants can affect the health of the body. Therefore, eliminating ROS through diet has become a relatively ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06A23L33/125A23L29/30
CPCA23L29/30A23L33/125C08B37/0003
Inventor 于晓丹吴双艳郭洪波王琴李伟吕淑霞马镝林英张良
Owner SHENYANG AGRI UNIV
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