Feed enterococcus faecalis and livestock and poultry heat stress control microecological preparation and preparation method thereof
A technology of microecological preparations and Enterococcus faecalis, which is applied in the field of Chinese herbal medicine microecological preparations, can solve the problems of tea fermentation probiotic strains lacking tea polyphenols, failure to achieve application effects, tea polyphenols oxidation loss, etc., to extend shelf life time, increasing the content of free tea polyphenols, and reducing the loss of tea polyphenols
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Embodiment 1
[0028] Embodiment 1--Isolation and identification of Enterococcus faecalis BD-1
[0029] 1. Isolation, enrichment and purification of Enterococcus faecalis BD-1
[0030] Under aseptic operation conditions, farmers in Xia Village, Zherong County, Ningde City, Fujian Province (coordinates: 119 。 51′59.7″E, 27 。 13′34.0″N) Caecum mucosal segment of self-supporting healthy adult pigs, scrape the mucosal mucus with a sterilized glass slide and put it in the EP tube, dilute it with sterile normal saline for 10 2 Take 0.1mL and spread it on the MRS agar plate containing bromocresol purple indicator (0.01%, g / mL). The colonies that turned yellow were streaked and purified, and the strains that were positive for Gram staining under the microscope and without spores were selected to contain bromocresol purple (0.01%, g / mL), tea polyphenols (0.01%, g / mL) and garlic (0.01%, g / mL) on the MRS agar plate for re-screening, and finally select the colony with obvious yellow color, smooth and...
Embodiment 2
[0041] Embodiment 2--anaerobic and aerobic fermentation of Enterococcus faecalis BD-1 in MRS liquid medium
[0042] (1) Activation of Enterococcus faecalis BD-1: Wash the slant of Enterococcus faecalis BD-1 test tube with sterile saline in a sterile environment, wash off the bacterial lawn on the surface of the medium, and then inoculate it into MRS liquid medium medium, 37°C static culture for 48h;
[0043] (2) Anaerobic static fermentation of Enterococcus faecalis BD-1 in MRS liquid medium:
[0044] Inoculate the activation solution of Enterococcus faecalis BD-1 obtained in step (1) into an anaerobic serum bottle containing MRS liquid medium in an anaerobic box according to the inoculation amount of 3% (volume ratio), and incubate statically at 37°C for 48 hours. At the end of the culture, the number of viable bacteria and the pH value of the fermentation broth were measured;
[0045] (3) Aerobic fermentation of Enterococcus faecalis BD-1 in MRS liquid medium:
[0046] Pu...
Embodiment 3
[0049] Embodiment 3--The effect of Pseudostellaria radix extract on the growth and pH of Enterococcus faecalis BD-1
[0050] (1) Activation of Enterococcus faecalis BD-1: same as step (1) in Example 2;
[0051] (2) Inoculate the activation solution of Enterococcus faecalis BD-1 obtained in step (1) into an anaerobic serum bottle containing MRS liquid medium in an anaerobic box according to the inoculation amount of 3% (volume ratio), in which the anaerobic serum The gradient of Pseudostellaria Pseudostellaria extract in the MRS liquid medium in the bottle was set in sequence (g / mL): 0%, 0.10%, 0.25%, 0.5%, and cultured at 37°C for 48 hours, and the pH of the fermentation broth was measured at the end of the culture value and the number of live bacteria, the results are shown in Table 3;
[0052]
[0053] (3) Inoculate the activation solution of Enterococcus faecalis BD-1 obtained in step (1) into a 500 mL Erlenmeyer flask containing 100 mL of MRS liquid medium according to...
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