Porcine reproductive and respiratory syndrome, swine mycoplasmal pneumonia combined live vaccine and preparation method thereof

A technology for swine mycoplasma pneumonia and respiratory syndrome, which is applied in the direction of pharmaceutical formulations, bacterial antigenic components, viral antigenic components, etc., can solve the problems of decreased immune effect, animal stress and safety issues, and achieve simple preparation methods and strengthen mutual immunity Effect, the effect of high vaccine titer

Inactive Publication Date: 2017-11-17
NANJING DAYAO NETWORK TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Mixed infection of the above two diseases requires multiple injections for a single vaccine, which often brings stress and safety problems to animals, and the immune effect will also decrease

Method used

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  • Porcine reproductive and respiratory syndrome, swine mycoplasmal pneumonia combined live vaccine and preparation method thereof
  • Porcine reproductive and respiratory syndrome, swine mycoplasmal pneumonia combined live vaccine and preparation method thereof
  • Porcine reproductive and respiratory syndrome, swine mycoplasmal pneumonia combined live vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 mycoplasma pneumonia live vaccine (168 strains) antigen and porcine reproductive and respiratory syndrome live vaccine (R98 strain) antigen

[0040] 1. Antigen Preparation of Live Vaccine of Mycoplasma Porcine Pneumonia (168 strains)

[0041] 1.1 Source of the strain:

[0042] The 168 strains of Mycoplasma hyopneumoniae used in the manufacture and testing of this product were provided by Guizhou First Biotechnology Co., Ltd.

[0043]1.2 Preparation of seeds for production Take freeze-dried strains and inoculate them in KM at a volume ratio of 1:100-1000 2 In the culture medium, culture at 37°C for about 3 to 7 days. When the culture changes color and is slightly turbid, take a culture smear for Wright staining and microscopic examination. When the bacterial morphology meets the standard and the pure inspection is qualified, expand the culture. . The number of succession does not exceed 5 generations.

[0044] 1.3 Medium KM for making ...

Embodiment 2

[0059] The preparation of embodiment 2 porcine reproductive and respiratory syndrome (R98 strain), swine mycoplasma pneumonia (168 strain) dual live vaccine

[0060] 1. Vaccine lyoprotectant treatment

[0061] Sucrose-gelatin adjuvant: Dissolve 1.5% gelatin and 12.5% ​​sucrose in water, autoclave at 115°C for 30 minutes, adjust the pH to 7.0 with sterile NaOH, and place in a greenhouse at 37°C for later use.

[0062] 2. Matching seedlings

[0063] Porcine reproductive and respiratory syndrome (R98 strain) antigen and porcine mycoplasma pneumonia (168 strain) antigen were mixed in a ratio of 1:1 by aseptic operation, then added with the same volume of sucrose gelatin adjuvant, and mixed evenly. Make porcine reproductive and respiratory syndrome (R98 strain) antigen content ≥ 10 5.0 TCID 50 / head, mycoplasma pneumonia (168 strains) antigen content ≥ 10 6.0 CCU / Touquan.

[0064] 3. Subpackaging and freeze-drying

[0065] The uniformly mixed vaccine is prepared and the spec...

Embodiment 3

[0067] Example 3 Different antigen content porcine reproductive and respiratory syndrome (R98 strain), swine mycoplasma pneumonia (168 strain) dual live vaccine efficacy test

[0068] 1. Test material

[0069] Have been divided into groups in embodiment 2, vaccine 1 (porcine reproductive and respiratory syndrome (R98 strain) 10 4 TCID 50 / Toufen + Mycoplasma pneumoniae (168 strains) 10 5 CCU / head portion), vaccine 2 (porcine reproductive and respiratory syndrome (R98 strain) 10 5 TCID 50 / Toufen + Mycoplasma pneumoniae (168 strains) 10 6 CCU / head portion) and vaccine 3 (porcine reproductive and respiratory syndrome (R98 strain) 10 6 TCID 50 / Toufen + Mycoplasma pneumoniae (168 strains) 10 7 CCU / Toufen)

[0070] 1-2 week-old Bama pigs free of Mycoplasma hyopneumoniae and PRRS antibodies and antigens.

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Abstract

The invention relates to porcine reproductive and respiratory syndrome, swine mycoplasmal pneumonia combined live vaccine and a preparation method thereof, which belong to the technical field of a veterinary biological product. The vaccine contains swine mycoplasmal pneumonia live vaccine antigen with immunization amount and porcine reproductive and respiratory syndrome with immunization amount, a freeze-drying protective additive is added, and a step of freeze drying is carried out. The antigenic component of the combined live vaccine are non-mutual interference or non-mutual influence, and mutually enhance the immunization effect, the primary immunization can reach the immunization protection, and the method has the advantages of good security, simple preparation method, convenient and fast immunization, and reduced immunization cost.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, and relates to a dual live vaccine of porcine reproductive and respiratory syndrome and swine mycoplasma pneumonia and a preparation method thereof. Background technique [0002] Porcine reproductive and respiratory syndrome, also known as porcine blue ear disease, is caused by porcine reproductive and respiratory syndrome virus. It is clinically characterized by fever, abortion in sows, increased mortality of piglets before and after weaning, and respiratory disorders in pigs of different ages. disease. It is an enveloped, single-stranded positive-sense RNA virus with two serotypes, American and European. At present, the disease is an important disease that seriously affects the development of the pig industry. Vaccination is the most effective way to prevent the disease. [0003] Mycoplasma hyopneumonia, also known as swine asthma, is a contact chronic respiratory infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/12A61K39/02A61K9/19A61K47/26A61K47/42A61P31/04A61P31/14
Inventor 朱燕锋卢业云杨德明
Owner NANJING DAYAO NETWORK TECH CO LTD
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