Thermophilic C-phycocyanin and method for extracting thermophilic C-phycocyanin from synechococcus cyanobacteria

A phycocyanin and cyanobacteria technology, applied in the field of thermophilic C-phycocyanin, can solve problems such as low temperature tolerance, and achieve the effect of significant application advantages

Inactive Publication Date: 2017-11-17
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the C-phycocyanin currently used in the market comes from spirulina, and most of them are room temperature proteins, which have relatively low temperature tolerance, which leads to defects in some high-temperature or temperature-requiring industrial fields.

Method used

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  • Thermophilic C-phycocyanin and method for extracting thermophilic C-phycocyanin from synechococcus cyanobacteria
  • Thermophilic C-phycocyanin and method for extracting thermophilic C-phycocyanin from synechococcus cyanobacteria
  • Thermophilic C-phycocyanin and method for extracting thermophilic C-phycocyanin from synechococcus cyanobacteria

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Embodiment 1

[0031] This embodiment provides a thermophilic C-phycocyanin, which has the amino acid sequence shown in Sequence 1 and figure 1 in the structure.

[0032] It is worth noting that the thermophilic C-phycocyanin of this example has a significantly different amino acid sequence from the thermophilic phycocyanin of P72509 queried by NCBI (ie, the National Center for Biotechnology Information, USA), which is especially shown in Amino acids at the 6 characteristic positions at positions 21, 28, 33, 37, 42 and 145; in the thermophilic C-phycocyanin of this embodiment, the amino acids at the above 6 characteristic positions correspond to asparagine (Asn, N), aspartic acid (Asp, D), arginine (Arg, R), serine (Ser, S), arginine (Arg, R) and glutamine (Gln, Q), while in P72509 In the thermophilic phycocyanin, the amino acids at the above six characteristic positions correspond to serine (Ser, S), phenylalanine (Phe, F), glutamine (Gln, Q), glycine (Gly, G) , lysine (Lys, K) and aspart...

Embodiment 2

[0066] In the description of Embodiment 2, the similarities with Embodiment 1 will not be repeated here, and only the differences with Embodiment 1 will be described. The difference between embodiment 2 and embodiment 1 is that in step S1, Synechococcus cyanobacteria are placed in a mixed solution of Bg-11 medium and water-soluble bicarbonate solution for cultivation; in other words, In Example 2, the mixed solution of Bg-11 medium and water-soluble bicarbonate solution is used as the compound medium; the rest refer to the description in Example 1 to obtain the amino acid sequence as shown in Sequence 1 and figure 1 The structure of thermophilic C-phycocyanin.

[0067] Preferably, the concentration of the water-soluble bicarbonate solution is 0.1mol L -1 ~0.3mol·L -1 ; Preferably 0.1mol L in the present embodiment -1 Sodium bicarbonate solution.

[0068] During the extraction process of the thermophilic C-phycocyanin of the present embodiment, the change of the absorbance ...

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Abstract

The invention discloses thermophilic C-phycocyanin. The thermophilic C-phycocyanin has an amino acid sequence which is shown as a first sequence, and amino acid at three or less characteristic positions of the 21th, 28th, 33th, 37th, 42th and 145th positions of amino acid sequence can be adjusted. The invention further provides a method for extracting the thermophilic C-phycocyanin from synechococcus cyanobacteria. According to the method for extracting the thermophilic C-phycocyanin from synechococcus cyanobacteria, the obtained thermophilic C-phycocyanin has good thermal stability and acid-base tolerance; compared with the phycocyanin which is extracted through spirulina and the like in the prior art, the thermophilic C-phycocyanin has good thermal stability and acid-base tolerance so that the thermophilic C-phycocyanin can have wider application range in the industrial application and shows more remarkable application advantages.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation engineering, and in particular relates to a thermophilic C-phycocyanin and a method for extracting the thermophilic C-phycocyanin from Synechococcus cyanobacteria. Background technique [0002] Synechococcus cyanobacteria are single-celled cyanobacteria, one of the most important species of the phylum Cyanobacteria, and are widely studied worldwide. Synechococcus and Prochlorococcus are the most abundant members of water microalgae (concentrations up to 10 5 cells mL -1 ~10 6 cells mL -1 ), and is considered to be the CO in the ocean area 2 Pin the most important players. Synechococcus can exist in a variety of habitats, including freshwater, seawater, and brackish water environments. The optimal growth temperature of some Synechococcus species is 55°C-60°C, and the cyanobacteria grown in such a high-temperature environment are still rich in a very important phycobiliprotein. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/14C12N1/20C12R1/01
CPCC07K14/795C12N1/20
Inventor 梁园梅江东毛睿慈
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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