Method for quickly screening pathogenic microorganisms in haze
A technology for pathogenic microorganisms and haze, applied in the directions of microorganism-based methods, biochemical equipment and methods, and microorganism determination/inspection, etc., can solve the problems of increasing the number of microorganisms, weakening the intensity of ultraviolet rays, weakening the sterilization effect, etc., to overcome the time-consuming , sample size increase, the effect of fast process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] Taking the haze air of Huayuan Farmers Market in Nankai District as a sample, the method of the present invention is used to quickly screen 7 kinds of pathogenic microorganisms. The preparation method is as follows:
[0042] (1) Using a six-stage filtration mode air microbial sampler, collect the microbes in the haze at a fixed point within 20 minutes, and culture the collected plates in a 36°C incubator overnight;
[0043] (2) Elute the bacterial lawn with 1 mL of sterile phosphate buffer in a 2 mL centrifuge tube, centrifuge at 13200 rpm for 10 minutes, discard the supernatant, and dissolve the precipitate in 100 μL deionized water, seal the membrane, and then boil it in a boiling water bath for 10 minutes. Frozen in the refrigerator at -20°C for 10 minutes, remove the sealing film, centrifuge at 13200 rpm for 3 minutes, the supernatant is DNA;
[0044] Real-time fluorescent PCR detection:
[0045] 1. Real-time fluorescent PCR amplification of 7 food-borne pathogenic bacteri...
Embodiment 2
[0073] Taking the haze air from the Jizhuangzi Farmers Market in Hexi District as a sample, the method of the present invention is used to quickly screen 7 kinds of pathogenic microorganisms. The preparation method is as follows:
[0074] (1) Using a six-stage filtration mode air microbial sampler, collect the microbes in the haze at a fixed point within 20 minutes, and culture the collected plates in a 36°C incubator overnight;
[0075] (2) Elute the bacterial lawn with 1 mL of sterile phosphate buffer in a 2 mL centrifuge tube, centrifuge at 13200 rpm for 10 minutes, discard the supernatant, and dissolve the precipitate in 100 μL deionized water, seal the membrane, and then boil it in a boiling water bath for 10 minutes. Frozen in the refrigerator at -20°C for 10 minutes, remove the sealing film, centrifuge at 13200 rpm for 3 minutes, the supernatant is DNA;
[0076] Real-time fluorescent PCR detection:
[0077] 1. Real-time fluorescent PCR amplification of 7 food-borne pathogenic ...
Embodiment 3
[0105] Taking the haze air at the Jinjing Highway toll station in Xiqing District as a sample, the method of the present invention is used to quickly screen 7 kinds of pathogenic microorganisms. The preparation method is as follows:
[0106] (1) Using a six-stage filtration mode air microbial sampler, collect the microbes in the haze at a fixed point within 20 minutes, and culture the collected plates in a 36°C incubator overnight;
[0107] (2) Elute the bacterial lawn with 1 mL of sterile phosphate buffer in a 2 mL centrifuge tube, centrifuge at 13200 rpm for 10 minutes, discard the supernatant, and dissolve the precipitate in 100 μL deionized water, seal the membrane, and then boil it in a boiling water bath for 10 minutes. Frozen in the refrigerator at -20°C for 10 minutes, remove the sealing film, centrifuge at 13200 rpm for 3 minutes, the supernatant is DNA;
[0108] Real-time fluorescent PCR detection:
[0109] 1. Real-time fluorescent PCR amplification of 7 food-borne pathogen...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More