Preparation method of carbon quantum dots and application of carbon quantum dots in biofilm imaging

Abstract

The invention belongs to the field of biotechnologies and nano materials and provides a preparation method of carbon quantum dots and application of the carbon quantum dots in biofilm imaging. The carbon quantum dots are prepared by taking lactobacillus plantarum fermentation liquid as a raw material through a hydrothermal reaction one-step method. The carbon quantum dots adopt a simple preparation technology, are relatively low in cost, environmentally friendly and easy to implement; the quantum yield of the prepared carbon quantum dots can reach 12%; and the prepared carbon quantum dots have the advantages of relatively high photostability, good water solubility and the like and can be applied to biofilm microbial imaging. Compared with a commercialized biofilm microbial dye, the biofilm microbial imaging process of the carbon quantum dots has the advantages that the carbon quantum dots do not need to be kept in a dark place, are short in incubating time and free of cleaning, long-term imaging observation can be achieved, a biofilm is not destroyed, and red and green double-channel imaging can be carried out at the same time.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and in particular relates to a preparation method of carbon quantum dots using Lactobacillus plantarum fermentation broth as a raw material, and the application of the carbon quantum dots in microbial biofilm imaging. Background technique [0002] Biomembranes are dense network structures composed of exopolysaccharides, proteins, nucleic acids, and even lipids. The formation of biofilm is a dynamic process, which mainly includes the following four stages: the colonization stage of reversible microbial adhesion, the accumulation stage of irreversible adhesion, the maturation stage of biofilm, and the shedding and recolonization stage of microorganisms. Biofilm is ubiquitous and involves all aspects of human existence. Once biofilm is formed, it will cause cross-contamination of food, medical supplies, food and pharmaceutical processing environments, and equipment. The formation of b...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing carbon quantum dots using Lactobacillus plantarum fermentation liquid as a raw material, and use it as a fluorescent probe for biofilm imaging to solve the problem of high cost of traditional commercial biofilm dyes and the need for cleaning and incubation Long time, destruction of biofilm and other problems, providing a biofilm dye with low price and easy operation

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