Preparation method of carbon quantum dots and application of carbon quantum dots in biofilm imaging

A technology of carbon quantum dots and microbial biofilms, which is applied in the field of nanomaterials and biology, can solve the problems of destroying biofilms, long incubation time, and the need for cleaning, and achieve the effects of short fermentation time, short incubation time, and low cost

Active Publication Date: 2017-12-01
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing carbon quantum dots using Lactobacillus plantarum fermentation liquid as a raw material, and use it as a fluorescent probe for biofilm imaging to solve the problem of high cost of traditional commercial biofilm dyes and the need for cleaning and incubation Long time, destruction of biofilm and other problems, providing a biofilm dye with low price and easy operation

Method used

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  • Preparation method of carbon quantum dots and application of carbon quantum dots in biofilm imaging
  • Preparation method of carbon quantum dots and application of carbon quantum dots in biofilm imaging
  • Preparation method of carbon quantum dots and application of carbon quantum dots in biofilm imaging

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Experimental program
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Embodiment 1

[0041] The preparation of embodiment 1 lactobacillus plantarum fermented liquid

[0042] Lactobacillus plantarum LCC-605 preserved in a glycerol tube is inoculated in a liquid MRS medium at a ratio of 1-3% by volume, placed at 25-42°C for static culture for 12-24 hours, and the obtained fermentation broth is set aside.

Embodiment 2

[0043] The preparation of embodiment 2 carbon quantum dot solution

[0044] Get 30mL of the fermented liquid prepared in Example 1 and place it in a 100mL hydrothermal reactor. Put it in an oven at 120-240°C, and react for 12-48 hours. See Table 1 for specific reaction conditions. After the reaction, the residue was removed, and the remaining liquid was filtered for later use. When the reaction temperature is 200°C and the reaction time is 24h, the quantum yield of the obtained carbon quantum dots is 12%.

[0045] Table 1 Preparation of carbon quantum dots under different reaction conditions

[0046] serial number

Reaction temperature (°C)

Reaction time (h)

1

120

48

2

200

24

3

240

12

[0047] Quantum yield measurement method:

[0048] Measure the absorbance of the carbon quantum dot solution to be measured and the reference substance quinine sulfate, measure the fluorescence intensity of the carbon quantum dot solut...

Embodiment 3

[0051] Transmission electron microscope observation of embodiment 3 carbon quantum dots

[0052] Transmission electron microscope observation of the carbon quantum dots prepared in Example 2: the filtered carbon quantum dot solution was diluted 10 times with deionized water, and 10uL was dropped on a 400-mesh copper grid, and a transmission electron microscope (JEM-2100, JEOL Ltd., Japan) for observation. The results show that the carbon quantum dots have an approximate spherical structure ( figure 1 ), the distribution is uniform, and the average particle size is about 1.6nm ( figure 2 ).

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Abstract

The invention belongs to the field of biotechnologies and nano materials and provides a preparation method of carbon quantum dots and application of the carbon quantum dots in biofilm imaging. The carbon quantum dots are prepared by taking lactobacillus plantarum fermentation liquid as a raw material through a hydrothermal reaction one-step method. The carbon quantum dots adopt a simple preparation technology, are relatively low in cost, environmentally friendly and easy to implement; the quantum yield of the prepared carbon quantum dots can reach 12%; and the prepared carbon quantum dots have the advantages of relatively high photostability, good water solubility and the like and can be applied to biofilm microbial imaging. Compared with a commercialized biofilm microbial dye, the biofilm microbial imaging process of the carbon quantum dots has the advantages that the carbon quantum dots do not need to be kept in a dark place, are short in incubating time and free of cleaning, long-term imaging observation can be achieved, a biofilm is not destroyed, and red and green double-channel imaging can be carried out at the same time.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and in particular relates to a preparation method of carbon quantum dots using Lactobacillus plantarum fermentation broth as a raw material, and the application of the carbon quantum dots in microbial biofilm imaging. Background technique [0002] Biomembranes are dense network structures composed of exopolysaccharides, proteins, nucleic acids, and even lipids. The formation of biofilm is a dynamic process, which mainly includes the following four stages: the colonization stage of reversible microbial adhesion, the accumulation stage of irreversible adhesion, the maturation stage of biofilm, and the shedding and recolonization stage of microorganisms. Biofilm is ubiquitous and involves all aspects of human existence. Once biofilm is formed, it will cause cross-contamination of food, medical supplies, food and pharmaceutical processing environments, and equipment. The formation of b...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/65G01N21/6428G01N2021/6439
Inventor 林凤鸣李程程
Owner SOUTHEAST UNIV
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