Method for promoting germination of Cynanchum acuminata seeds through fungi

A technology of fungi and seeds, which is applied in the field of promoting Cynomorium seed germination, can solve the problems of affecting seed germination, long cycle of Cynomorium seed germination, and high reagent cost, so as to promote the germination of Cynomorium seed, accelerate the rapid reproduction of Cynomorium, improve The effect of seed germination rate

Active Publication Date: 2017-12-05
SHANXI UNIV
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Problems solved by technology

(4) The presence of host plant inducers such as strigolactone is the fourth major difficulty affecting seed germination
[0004] Up to now, there have been reports on promoting the germination of Cynomorium seeds by means of physical, chemical and biological hormones. Compared with natural germination, the germination efficiency of seeds has been effectively improved. Long, high cost of reagents in the germination method, polluting seeds and the environment, complex operation, risk factors, poor sustainability, etc.

Method used

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  • Method for promoting germination of Cynanchum acuminata seeds through fungi

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the influence of fermented liquid stoste on Cynomorium cynomorium seed germination

[0020] 1) Surface treatment of Cynomorium seeds

[0021] Pick plump wild Cynomorium seeds, soak them in 1mol / L sodium hydroxide solution for 5 hours, then filter off the solution to obtain peeled Cynomorium seeds; immerse the peeled Cynomorium seeds into 80% methanol solution , stirred at 25°C for 24h, filtered out the methanol solution, and rinsed Cynomorium seeds repeatedly with sterile water.

[0022] 2) Preparation of strains and their fermentation broth stock solution

[0023] Under sterile conditions, culture CGMCC No.13875 fungus on a PDA plate at 25-30°C for 5 days, take 4 pieces of 0.5cm bacterial blocks from the edge of the colony, and then inoculate them into 250mL conical tubes filled with 100ml of culture solution. In the bottle, ensure that the bacteria blocks are dispersed in the culture medium, keep the constant temperature at 25°C, shake the table at 180...

Embodiment 2

[0027] Embodiment 2: the influence of fermented liquid stoste concentrate on Cynomorium cynomorium seed germination

[0028] 1) the surface treatment of Cynomorium seed, with embodiment 1;

[0029] 2) According to step 2) of Example 1, prepare strains and their fermented broth stock solution, and concentrate the obtained fermented broth stock solution to 0.5 times the original volume (concentrated solution);

[0030] 3) Put the cynomorium seeds treated in step 1) evenly in a petri dish (n=3) covered with two layers of sterile filter paper, and add the above-mentioned concentrated solution dropwise at 8:00 am and 8:00 pm every day to keep the filter paper moist. Wet, cultivated in dark at a temperature of 28°C, regularly observe, record and calculate the germination time and germination rate of Cynomorium cynomorium seeds.

[0031] After 5 days, the germination rate reached 48.72%, see Table 1.

Embodiment 3

[0032] Embodiment 3: the influence of fermented liquid stoste dilution liquid on Cynomorium cynomorium seed germination

[0033] 1) the surface treatment of Cynomorium seed, with embodiment 1;

[0034] 2) According to step 2) of Example 1, prepare the bacterial strain and its fermented broth stock solution, and dilute the obtained fermented broth stock solution with 0.5 times the volume of the diluted solution with sterile water.

[0035] 3) Put the Cynomorium yang seeds treated in step 1) evenly in a petri dish (n=3) covered with two layers of sterile filter paper, and add the above-mentioned dilution solution dropwise at 8:00 am and 8:00 pm every day to keep the filter paper moist. Wet, cultivate in dark under the condition of temperature 28°C, regularly observe, record and calculate the germination time and germination rate of Cynomorium seeds

[0036] After 10 days, the germination rate reached 35.7%, see Table 1.

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Abstract

The invention provides a method for promoting germination of Cynanchum acuminata seeds through fungi and concretely provides a method for inducing fast germination of Cynanchum acuminata seeds through a fermentation broth of Fusarium redolens having the preservation number CGMCC No. 13875. When the fermentation broth of Fusarium redolens is concentrated to 1 / 2 the original volume, the germination rate is the highest and is 48.72% and germination time is only 5 days. The method is simple in operation and low in cost, has no pollution and is an effective method for promoting rapid germination of Cynanchum yunnanensis seeds.

Description

technical field [0001] The invention relates to the breeding technology of Chinese herbal medicine, and specifically belongs to a method for promoting the germination of Cynomorium cynomorium seeds by using the fermented liquid of Nitraria endophytic fungi. Background technique [0002] Cynomorium songaricum is a single-family, single-genus, and single-species plant of Cynomoriaceae (Cynomoriaceae), which mostly parasitizes the roots of plants of the genus Nityaria (Zygophyllaceae) and is a specific all-parasitic seed. Plants are mainly distributed in Gansu, Xinjiang, Qinghai, Inner Mongolia and other provinces. Lock Yang is warm, sweet, and has the effects of nourishing kidney yang, benefiting essence and blood, moistening intestines and laxative. In recent years, studies have shown that Cynomorium also has important medical value in cancer prevention, anti-cancer, immune regulation, anti-aging, prevention and treatment of cardiovascular diseases, and treatment of leukopen...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01N63/04A01P21/00C12N1/14C12R1/77
CPCA01C1/00A01N63/30C12N1/14C12N1/145C12R2001/77
Inventor 崔晋龙张艳艳
Owner SHANXI UNIV
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