Embedding method for oxytocin based on squid cuttlebone
The technology of sea octopus and hormones is applied in the directions of pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc. and other problems, to achieve the effect of long anesthesia maintenance time, long release time and reducing stress response
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Embodiment 1
[0014] An oxytocin embedding method based on cuttlebone octopus, comprising the following steps: first, anesthesia: scoop out the squid with a dipping net, place it in an anesthetic container for 10 minutes, and observe the anesthesia of the squid at all times; then embedding : Use a sterilized knife to cut open the muscular mantle of the squid, the length of the opening is about 25mm, use a twist drill to drill a hole on the exposed seaweed octopus, put the hormone slow-release agent in, and transfer the squid to fresh in sea water. The preparation method of the hormone sustained-release agent is as follows: take 10 parts of chitosan quaternary ammonium salt in a container, add 900 parts of distilled water, stir for 10 minutes, then add 0.1 parts of GnRH powder, stir for 10 minutes, then add 5 parts of azobis Formamide, stirred for 4 minutes, then added 2 parts of sodium tripolyphosphate, stirred for 30 minutes, set the relative centrifugal force of the centrifuge to 2000g, c...
Embodiment 2
[0016] A method for embedding oxytocin based on squid cuttlebone, comprising the following steps:
[0017] 1) Anesthesia: place the squid in an anesthetic for 9 minutes. The prepared anesthetic induces rapidly and smoothly, and maintains anesthesia for a long time. ;
[0018] 2) Embedding: cut the muscular mantle of the squid, drill holes in the exposed cuttlebone, put the hormone slow-release agent in, and transfer the squid into fresh seawater after 1 min. The preparation method of the hormone sustained-release agent is: take 15 parts of chitosan quaternary ammonium salt, add 1000 parts of distilled water, stir evenly, then add 0.2 parts of GnRH powder, stir for 15 minutes, then add 7 parts of azodicarbonamide, stir After 6 minutes, 3 parts of sodium tripolyphosphate were added, stirred for 35 minutes, the liquid was ultracentrifuged, and the obtained solid mixture was freeze-dried to obtain a hormone sustained-release preparation. Squid octopus is a calcareous endoskelet...
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