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Embedding method for oxytocin based on squid cuttlebone

The technology of sea octopus and hormones is applied in the directions of pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc. and other problems, to achieve the effect of long anesthesia maintenance time, long release time and reducing stress response

Active Publication Date: 2017-12-15
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The prior art, such as the Chinese invention patent application publication number CN 106719178 A, discloses a method for artificially promoting maturation of cephalopods. In this method, oxytocin hormone is implanted into the orbital sinuses of cephalopods, so that the hormone is released slowly and promotes cephalopod ripening. cephalopod breeding, but the preparation process of the hormone slow-release agent in this method is complicated, and the stress response of cephalopods is large during anesthesia

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  • Embedding method for oxytocin based on squid cuttlebone

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Embodiment 1

[0014] An oxytocin embedding method based on cuttlebone octopus, comprising the following steps: first, anesthesia: scoop out the squid with a dipping net, place it in an anesthetic container for 10 minutes, and observe the anesthesia of the squid at all times; then embedding : Use a sterilized knife to cut open the muscular mantle of the squid, the length of the opening is about 25mm, use a twist drill to drill a hole on the exposed seaweed octopus, put the hormone slow-release agent in, and transfer the squid to fresh in sea water. The preparation method of the hormone sustained-release agent is as follows: take 10 parts of chitosan quaternary ammonium salt in a container, add 900 parts of distilled water, stir for 10 minutes, then add 0.1 parts of GnRH powder, stir for 10 minutes, then add 5 parts of azobis Formamide, stirred for 4 minutes, then added 2 parts of sodium tripolyphosphate, stirred for 30 minutes, set the relative centrifugal force of the centrifuge to 2000g, c...

Embodiment 2

[0016] A method for embedding oxytocin based on squid cuttlebone, comprising the following steps:

[0017] 1) Anesthesia: place the squid in an anesthetic for 9 minutes. The prepared anesthetic induces rapidly and smoothly, and maintains anesthesia for a long time. ;

[0018] 2) Embedding: cut the muscular mantle of the squid, drill holes in the exposed cuttlebone, put the hormone slow-release agent in, and transfer the squid into fresh seawater after 1 min. The preparation method of the hormone sustained-release agent is: take 15 parts of chitosan quaternary ammonium salt, add 1000 parts of distilled water, stir evenly, then add 0.2 parts of GnRH powder, stir for 15 minutes, then add 7 parts of azodicarbonamide, stir After 6 minutes, 3 parts of sodium tripolyphosphate were added, stirred for 35 minutes, the liquid was ultracentrifuged, and the obtained solid mixture was freeze-dried to obtain a hormone sustained-release preparation. Squid octopus is a calcareous endoskelet...

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Abstract

The invention discloses an embedding method for oxytocin based on squid cuttlebone. Firstly, placing squids into a narcotic, and performing anaesthetization; and then embedding an oxytocin low-release agent into the cuttlebone. The method has the beneficial effects that the embedded oxytocin is located in calcareous cuttlebone of the squids, thus the embedded oxytocin has small stimulation effects on the squids, the cuttlebone has a loose and porous structure, and therefore the oxytocin can be easily released into bodies to play roles; and a certain amount of L-medetomidine is added to R-medetomidine, thus the prepared narcotic has long anaesthetization hold time and a certain analgesic effect, the stress reaction of the squids to an embedding process is greatly reduced, and ink spraying is avoided; and the GnRH low-release agent prepared by using chitosan quaternary ammonium salt as a carrier has good biocompatibility, long release time and a uniform release speed.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to an oxytocin embedding method based on squid cuttlefish and octopus. Background technique [0002] Squid was once one of the four major fisheries in my country's fishing grounds. It has strong adaptability to temperature and is widely distributed in my country's coastal areas. It is a traditional fishery species with high economic value. Due to overfishing and ecological deterioration, since the 1970s, there have been successive declines. In order to alleviate this dilemma, artificial breeding of squid is an effective way to solve the above problems. In recent years, the breeding and augmentation of squid has developed well in China, but the artificial breeding of squid still relies on its natural reproduction and spawning, and it is urgent to increase the number of squid through artificial induction. [0003] The prior art, such as the Chinese invention patent application p...

Claims

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Application Information

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IPC IPC(8): A01K61/00A61K38/09A61K47/36A61K47/18A61P15/08
CPCA01K61/00A61K9/0002A61K38/09A61K47/18A61K47/36Y02A40/81
Inventor 龚理窦灿丰刘立芹吕振明迟长凤王天明黄伟吴常文
Owner ZHEJIANG OCEAN UNIV
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