Composite bacterium agent and use for pyrethroid pesticide residues in degraded soil

The technology of a compound bacterial agent and fenvalerate, which is applied in the field of microorganisms, achieves the effects of reducing the dosage of drugs, quickly adapting to the environment, and being simple and easy to operate.

Active Publication Date: 2017-12-15
JIANGSU LV HUA BIOLOGY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In natural environments such as soil and water, the degradation of residual pesticides is often the result of the joint action of multiple microorganisms. However, most of the previous studies focused on the degradation of a single pesticide by a single bacterium, and the simultaneous degradation of multiple pyrethroids by mixed bacteria. Pesticides are less reported

Method used

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  • Composite bacterium agent and use for pyrethroid pesticide residues in degraded soil
  • Composite bacterium agent and use for pyrethroid pesticide residues in degraded soil
  • Composite bacterium agent and use for pyrethroid pesticide residues in degraded soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of composite bacterial agent, it is prepared according to the following steps:

[0034] Take fenvalerate 100mg, glucose 50g, crop straw powder 40g, soybean meal 20g, K 2 HPO 4 2g, KH 2 PO 4 2g, MgSO 4 0.5g, MnSO 4 0.2g, FeSO 4 Add 0.1g to water, adjust the pH to 6.8, and dilute to 1L to prepare the acclimatization medium;

[0035] Sphingomonas seed solution (1 × 10 8 individuals / ml) according to the inoculation amount of 5% (volume ratio), transferred to the acclimatization medium, and cultivated at 30°C for 6h; then the Bacillus licheniformis seed liquid (1×10 8 individual / ml), Rhodopseudomonas palustris seed solution (1×10 8 individual / ml) and Micrococcus luteus seed solution (1×10 8 individual / ml) were mixed according to the volume ratio of 3:2:2, and then transferred to the acclimatization medium according to the inoculation amount of 6% (volume ratio), cultivated at 30°C for 6h, and then added 0.02wt% fenvalerate; finally Enterobacter cloacae s...

Embodiment 2

[0040] A kind of composite bacterial agent, it is prepared according to the following steps:

[0041] Take fenvalerate 100mg, glucose 50g, crop straw powder 40g, soybean meal 20g, K 2 HPO 4 2g, KH 2 PO 4 2g, MgSO 4 0.5g, MnSO 4 0.2g, FeSO 4 Add 0.1g to water, adjust the pH to 6.8, and adjust the volume to 1L to prepare the acclimatization medium;

[0042] Sphingomonas seed solution (1 × 10 8 individuals / ml) according to the inoculation amount of 5% (volume ratio), transferred to the acclimatization medium, and cultivated at 30°C for 9h; then the Bacillus licheniformis seed solution (1×10 8 individual / ml), Rhodopseudomonas palustris seed solution (1×10 8 individual / ml) and Micrococcus luteus seed solution (1×10 8 individual / ml) were mixed according to the volume ratio of 5:3:3, and then transferred to the acclimatization medium according to the inoculation amount of 6% (volume ratio), cultivated at 30°C for 9h, and then added 0.02wt% fenvalerate; finally Enterobac...

Embodiment 3

[0047] Degradation test of pyrethroid pesticide residues in soil:

[0048] Sample selection: Sample 1: the contents of bifenthrin, fenpropathrin and cypermethrin are all 100mg / kg; sample 2: the contents of bifenthrin, fenpropathrin and cypermethrin are all 200mg / kg; sample 3: The contents of bifenthrin, fenpropathrin and cypermethrin were all 400mg / kg.

[0049] Operation process: Taking Example 1 as an example, add 100 g of the compound microbial agent into 1000 kg of soil sample, stir evenly, and ensure that the water content is about 20-30%. The contents of bifenthrin, fenpropathrin and cypermethrin were detected at 12h, 24h, 48h, 72h and 96h respectively. figure 1 It can be seen that the degradation effects of the compound bacterial agents on bifenthrin, fenpropathrin and cypermethrin are not much different, the degradation rate can reach more than 35% in 24 hours, and the degradation is basically completed in 96 hours; Figure 2-3 It can be seen that the degradation rate...

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Abstract

The invention belongs to the technical field of microorganism, and discloses a composite bacterium agent. The composite bacterium agent is prepared according to the following steps: 1, preparation of acclimation medium, 2, acclimation of strains, 3, preparation of carrier, and 4, stirring, drying and packing. According to the composite bacterium agent, the highly concentrated pyrethroid pesticide residues in soil can be degraded, the degradation speed is fast, and the application prospect is broad.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite bacterial agent and its application in degrading pyrethroid pesticide residues in soil. Background technique [0002] Pyrethroid pesticides are one of the main types of pesticides that replace organochlorine pesticides and other highly toxic and long-residue pesticides in China. Its varieties and usage are second only to organophosphorus pesticides, accounting for the second place in the insecticide market. Since the pyrethroid pesticides after the second generation are characterized by the decomposition of light and heat, the pyrethroid residues in the natural environment are mainly removed by the activities of microorganisms, so foreign workers have screened some strains that can degrade pyrethroids And further study the degradation mechanism of pesticides. In China, it mainly focuses on the research on the environmental behavior and residue dig...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36B09C1/10C12R1/10C12R1/01C12R1/38C12R1/265
CPCB09C1/10C12N1/20C12N1/36
Inventor 李金奎李夏琳李俊迪
Owner JIANGSU LV HUA BIOLOGY ENG
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