Medium without animal source components for cultivating human mesenchymal stem cells

Abstract

The invention relates to a medium without animal source components for cultivating human mesenchymal stem cells. The medium comprises the following components: 1-100[mu]g / ml of recombinant human insulin, 1-50ng / ml of basic fibroblast growth factor, 1-50[mu]g / ml of human transferrin, 1-80[mu]g / ml of seleninic acid, 1-300[mu]g / ml of serine, 1-200[mu]g / ml of choline chloride, 1-60[mu]g / ml of adenine, 1-100[mu]g / ml of single acetamide, 0.01-10mmol / ml of o-phosphorylethanolamine 1-40pg / ml of progesterone, 1-50pg / ml of three iodine thyroid, 1-100[mu]l / ml of human blood plasma. The medium has substantially better culture efficiency than traditional mediums in cultivating human adipose-derived stem cells and human umbilical cord mesenchymal stem cells, and the medium has characteristics of maintenance of good stem cell characters, cell proliferation capability, and high biological performance; the medium reduces dependence of stem cells for fetal calf serum, reduces bad influences of fetal calf serum for stem cell characteristics and differentiation, thoroughly removes fetal calf serum, avoids pollution risk of virus due to bad quality of animal serum, and more accords with clinic applications.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to an animal-source component-free culture medium for culturing human mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells [mesenchymal stem cells, MSC] is an important member of the stem cell family, derived from the mesoderm and ectoderm in the early development, and belongs to pluripotent stem cells. MSC was first discovered in the bone marrow because of its multi-directional differentiation potential, hematopoietic Supporting and promoting stem cell engraftment, immune regulation and self-replication have attracted increasing attention. For example, mesenchymal stem cells can be differentiated into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells under specific induction conditions in vivo or in vitro, after continuous subculture and cryopreservation It still has multi-directional ...

AI Technical Summary

Problems solved by technology

However, these media are expensive, and it is necessary to use auxiliary products to coat the cell culture bottles during the process of culturing MSCs

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