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Method for screening and separating chlorine-resistant bacteria and Gordonia terrae strain obtained with same

A separation method and chlorine-resistant technology, applied in the directions of microorganism-based methods, separation microorganisms, biochemical equipment and methods, etc., can solve problems such as screening and separation of chlorine-resistant bacteria, and achieve the effect of ensuring biological safety.

Active Publication Date: 2017-12-22
SHANDONG WATER & WASTEWATER MONITORING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem that the current inspection method for the total number of bacterial colonies cannot screen and isolate chlorine-resistant bacteria in water, and provides a method for screening and separating chlorine-resistant bacteria. Chlorine screening, isolation and purification of chlorine-resistant bacteria, chlorine-resistant verification and other steps to screen and isolate chlorine-resistant bacteria in the water supply system

Method used

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  • Method for screening and separating chlorine-resistant bacteria and Gordonia terrae strain obtained with same
  • Method for screening and separating chlorine-resistant bacteria and Gordonia terrae strain obtained with same
  • Method for screening and separating chlorine-resistant bacteria and Gordonia terrae strain obtained with same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Taking pipe network water as an example, screening and separating chlorine-resistant bacteria therein

[0031] (1) Take 1L of water sample from a pipe network, enrich and concentrate the water sample through a 0.22μm polycarbonate membrane, then add 5mL of normal saline to elute, centrifuge at 3500rpm, discard the supernatant, collect the bacteria, and the collected bacteria Add 5 mL of nutrient broth and place in a water-proof incubator at 37°C for 24 hours. Centrifuge the cultured bacterial solution at 3500 rpm for 10 min, discard the supernatant, add 10 mL of normal saline and vortex to prepare a bacterial suspension for later use.

[0032] (2) Chlorination test was carried out on the bacterial suspension, the theoretical amount of chlorine added was 9mg / L, and HPC was measured at 30min, 1h, and 2h respectively to ensure that the residual chlorine in water was greater than 0.5mg / L during HPC measurement. Using R 2 A medium plate coating method was used fo...

Embodiment 2

[0044] This specific example uses the following steps:

[0045] (1) Weigh 10g of biological sand in a sand filter tank of a water plant, add 50mL of normal saline, and then 40KHz ultrasonic elution for 10min, 800rpm, centrifuge for 10min, take the supernatant and repeat the above centrifugation steps twice, take the supernatant and centrifuge at 3500rpm for 10min and discard it Add 10 mL of normal saline to the supernatant and vortex to prepare a bacterial suspension for later use.

[0046] (2) Carry out chlorination test to bacterial suspension, the theoretical concentration of chlorine after chlorination is 9mg / L, carry out HPC measurement respectively in action 30min, 1h, 2h, guarantee that the residual chlorine amount in water is greater than 0.5mg / L when HPC is measured .

[0047] (3) Using R 2 A medium plate coating method is used for HPC determination. After the coated plate is placed at 22°C for 7 days, the growth of the colony on the plate is observed, and the morph...

Embodiment 3

[0052] This specific example uses the following steps:

[0053] (1) Weigh 10g of biological activated carbon from a charcoal filter of a water plant, add 50mL of normal saline, then 40KHz ultrasonic elution for 10min, 800rpm, centrifuge for 10min, take the supernatant and repeat the above centrifugation steps twice, take the supernatant and centrifuge at 3500rpm for 10min, discard the supernatant Add 10 mL of normal saline and vortex to prepare a bacterial suspension for later use.

[0054] (2) carry out chlorination test to bacterium suspension, the theoretical concentration of chlorine after chlorination is 9mg / L, carry out HPC measurement (attachment) in effect 30min, 1h, 2h respectively Figure 5 ), to ensure that the amount of residual chlorine in the water is greater than 0.5mg / L when HPC is measured.

[0055] (3) Using R 2 A medium plate coating method is used for HPC determination. After the coated plate is placed at 22°C for 7 days, the growth of the colony on the p...

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Abstract

The invention discloses a method for screening and separating chlorine-resistant bacteria and a Gordonia terrae strain JN724 obtained with the same. The method disclosed by the invention comprises the following steps: enriching thalli in a sample, and then with chlorine resistance screening, separating and purifying of chlorine-resistant bacteria, and verifying of chlorine resistance, screening and separating the chlorine-resistant bacteria in a water supply system, thereby further obtaining a chlorine-resistant strain, wherein the homology of the strain with multiple Gordonia strains is 100%. By screening and separating the chlorine-resistant bacteria, the quantity of bacteria in water and composition of bacterial population can be grasped more accurately, more accurate theoretical basis is provided for microorganism safety assessment of urban water supply, and support is provided for improvement of a water plant disinfection technology, so that biological safety of drinking water is better guaranteed.

Description

technical field [0001] The invention relates to a method for screening and isolating chlorine-resistant bacteria in a water supply system and a strain of Gordonella terrae isolated from pipe network water by the method. Background technique [0002] At present, chlorine disinfection is still a widely used disinfection process in domestic water plants. The process of adding chlorine is also a process of selecting the chlorine resistance of bacteria. Bacteria with high tolerance to chlorine are called "chlorine-resistant bacteria". The chlorine-resistant bacteria existing in the water supply system have great harm to the safety of drinking water. On the one hand, some chlorine-resistant bacteria themselves are pathogenic bacteria or conditional pathogenic bacteria, such as mycobacteria, Legionella, Pseudomonas aeruginosa, aureus Staphylococcus, etc.; on the other hand, chlorine-resistant bacteria lead to the failure of chlorine disinfection, triggering the regrowth of microorg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/02C12R1/01
CPCC12N1/02C12N1/20C12Q1/02C12N1/205C12R2001/01
Inventor 贾瑞宝孙韶华逯南南褚福敏许燕
Owner SHANDONG WATER & WASTEWATER MONITORING CENT
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