A complete set of molecular markers for detecting the chromosome arms of T. villosa and its application

A chromosomal arm, tufted wheat technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. Insufficient utilization, etc.

Active Publication Date: 2020-05-08
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, the research and utilization of diploid Trichotilloides is not enough and needs to be strengthened. For example, there are few reports on the cloning of specific DNA fragments on specific chromosomes and the development of molecular markers.
There have been some reports on the specific markers of T. villosa, but most of them are concentrated on chromosome 6VS. Currently, markers distributed on chromosomes 1V to 7V mainly include SSR, ISSR, and EST-STS markers. These markers are used to identify a large number of structural variant chromosomes of T. villosa , which is far from meeting the needs of a large number of molecular genetics and breeding studies on the transfer of excellent genes from Trichosanthes villosa to wheat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A complete set of molecular markers for detecting the chromosome arms of T. villosa and its application
  • A complete set of molecular markers for detecting the chromosome arms of T. villosa and its application
  • A complete set of molecular markers for detecting the chromosome arms of T. villosa and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1. Development of a set of primers for detecting the chromosomal arm of T. villosa

[0107] Trichophyllum for testing: Trichophyllum No.1026, Wheat-Macrophyllum 1V-7V addition line (DA1V-7V), Sclerophyllum amphiploid.

[0108] Transcriptome sequencing: the leaves of T. villosa were taken, RNA was extracted, and transcriptome sequencing was performed to obtain the transcriptome sequence.

[0109] Sequence Alignment: Submit the transcriptome sequence to https: / / urgi.versailles.inra.fr / blast / for comparison, and search for sequences mapped to the genomes of wheat groups 1-7.

[0110] Primer design: According to the sequence compared to the corresponding genome of wheat, primers were designed using primer premier 6, and the primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.

[0111] Preliminary screening of primers: using the genomic DNA of Chinese Spring, Wheat-Tympus villosa 1V-7V addition line (DA1V-7V), and T. villosa No.1026 as templates, P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a complete set of molecular markers for detecting the chromosome arms of Haynaldia villosa and application thereof. The invention provides the complete set of molecular markers for detecting the chromosome arms of Haynaldia villosa, and the complete set of molecular markers are composed of all the or a part of primer pairs (14 primer pairs as shown in sequence 1 to 28 in a sequence table) used for detecting 2V, 3V, 4V, 6V and 7V chromosome arms of Haynaldia villosa. According to the invention, RNA-seq technology is employed for acquiring the transcriptome sequences of Haynaldia villosa; primers are designed on the basis of the transcriptome sequences; and specific markers capable of accurately identifying each chromosome of Haynaldia villosa are screened out, and corresponding amplification primers are designed. Experimental results show that the complete set of primers can realize specific detection of each chromosome of Haynaldia villosa and are successively applied to detection of Haynaldia villosa chromosomes in Triticum durum-Haynaldia villosa amphidiploid and wheat hybrid and backcross generation materials. The complete set of molecular markers in the invention provide a more convenient and effective tool for further research and utilization of Haynaldia villosa.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a set of molecular markers for detecting chromosomal arms of T. villosa and an application thereof. Background technique [0002] Dasypyrum villosum (L.) P.Candargy (syn. Haynaldia villosa Schur) is an annual or perennial diploid plant (2n=2x=14, Sears will The genome is named VV). It originates in the northeastern part of the Mediterranean, from southern France in southern Europe to the Caspian Sea, southwestern Asia, Russia and the Caucasus, and is a weedy plant that grows in harsh, arid environments. It is named because of the clusters of hairs on the glume ridges and the tops of the lemmas. Dust wheat has the characteristics of resistance to stripe rust, leaf rust, stem rust, powdery mildew and wheat shuttle mosaic virus and other major wheat diseases and insect pests. It also has cold resistance, strong tillering ability, luxuriant growth, many small flowers, high protein conten...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/16
Inventor 叶兴国李仕金林志珊王静王坤杨王轲陈竞男杜丽璞刘畅
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products