Photoelectrochemical aptamer sensor and preparation method and application thereof

An aptamer sensor and photoelectrochemical technology, applied in the field of biosensors, can solve the problems such as the position shift of the valence band, the reduction of the redox ability of photogenerated electron holes, and the detection of unfavorable target molecules, so as to reduce the preparation steps and improve the sensitivity. Effect

Active Publication Date: 2021-05-21
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing g-C 3 N 4 Although semiconductor nanocomposites can enhance electron conversion efficiency to a certain extent, their simple type II structure makes the position of the valence band move up and the position of the conduction band move down, resulting in a decrease in the redox ability of photogenerated electron holes, which is not conducive to target molecules. detection

Method used

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  • Photoelectrochemical aptamer sensor and preparation method and application thereof
  • Photoelectrochemical aptamer sensor and preparation method and application thereof
  • Photoelectrochemical aptamer sensor and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044]A photoelectrochemical aptasensor, including a conductive glass electrode used as a working electrode in a three-electrode system, the reaction end surface of the conductive glass electrode is modified with a composite film, and the composite film is jointly modified by gold nanoparticles / ceria quantum dots Composed of graphite-phase carbon nitride nanosheet composite materials, the surface of the composite film is self-assembled with specific aptamer probes that recognize and capture target molecules (can be selected according to actual needs), in which gold nanoparticles / ceria quantum dots Co-modified graphitic carbon nitride nanosheet composites (CeO 2 QDs / Au / g-C 3 N 4 ), including graphite phase carbon nitride nanosheets loaded with ceria quantum dots, graphite phase carbon nitride nanosheets loaded with ceria quantum dots decorated with gold nanoparticles, graphite phase carbon nitride loaded with ceria quantum dots The nano-sheet is based on graphite-phase carbo...

Embodiment 2

[0064] A photoelectrochemical aptasensor for detecting microcystin (MC-LR), comprising a conductive glass electrode used as a working electrode in a three-electrode system, the reaction end surface of the conductive glass electrode is modified with a composite film, the composite film Composed of graphite-phase carbon nitride nanosheet composites co-modified by gold nanoparticles / ceria quantum dots, the surface of the composite film is self-assembled with specific aptamer probes that recognize and capture target molecules (MC-LR), Among them, the graphitic phase carbon nitride nanosheet composite material (CeO 2 QDs / Au / g-C 3 N 4 ), including graphite phase carbon nitride nanosheets loaded with ceria quantum dots, graphite phase carbon nitride nanosheets loaded with ceria quantum dots decorated with gold nanoparticles, graphite phase carbon nitride loaded with ceria quantum dots The nano-sheet is based on graphite-phase carbon nitride nano-sheet as a carrier, and the graphit...

Embodiment 3

[0085] In order to further verify the photoelectrochemical aptamer sensor (aptamer / CeO 2 QDs / Au / g-C 3 N 4 / ITO) detection effect in practical application, the photoelectrochemical aptamer sensor is used for target detection in actual samples (assay method with reference to embodiment 2), and the recovery rate experiment is carried out

[0086] Adopt photoelectrochemical aptamer sensor (aptamer / CeO2) in embodiment 2 2 QDs / Au / g-C 3 N 4 / ITO) to detect the concentration of MC-LR in Taozi Lake water, Dongting Lake water and laboratory tap water respectively. The specific steps are: after the above water samples are pretreated by filtration, etc., get the supernatant and adjust the pH to 7.5 with phosphate buffer solution. The concentration of the target substance in the sample (containing MC-LR) refers to Table 1, and finally the photoelectrochemical aptasensor is used for target detection in the actual sample (assay method refers to Example 2), and the recovery rate experim...

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Abstract

The invention discloses a photoelectrochemical aptamer sensor and a preparation method and application thereof. The sensor comprises a working electrode, the reaction end face of which is modified with a composite film, wherein the composite film is composed of a graphite phase carbon nitride nanosheet composite material jointly modified by gold nanoparticles/cerium dioxide quantum dots; and a specific aptamer probe is self-assembled on the surface of the composite film. The preparation method comprises the following steps: modification of CeO2QDs/Au/g-C3N4 and fixation of the specific aptamer probe. The photoelectrochemical aptamer sensor disclosed by the invention has the advantages of high stability, long service life, strong anti-interference capability, wide detection range, low detection limit and the like, can realize specific detection of target objects in media such as water bodies and organisms, and is high in utilization rate, high in use value and good in application prospect. The preparation method of the sensor has the advantages of simple process, convenience in operation, safety, low cost, no pollution, high manufacturing efficiency and the like, is suitable for large-scale preparation and is beneficial to industrial application.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a photoelectrochemical aptamer sensor and its preparation method and application, in particular to a graphite-phase carbon nitride nanosheet composite material based on gold nanoparticles / ceria quantum dots co-modified The photoelectrochemical aptasensor and its preparation method and application. Background technique [0002] Microcystins (MCs) are the most common cyclic heptapeptide toxins in the aquatic environment, among which microcystins (MC-LR) have the widest distribution range, the strongest toxicity and the highest frequency of occurrence. MC-LR will not only directly cause harm to aquatic organisms, but also enter farmland soil through fertilization, irrigation, etc., gradually accumulate in aquatic animals and plants, affect their body functions, cause them to be poisoned, and accumulate to animals, plants and humans along the food chain In the body, it mainly manif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327G01N27/36G01N33/543G01N33/531B82Y5/00B82Y30/00B82Y40/00
CPCG01N27/26G01N27/3278G01N27/36G01N33/5438G01N33/54393G01N33/531B82Y30/00B82Y40/00B82Y5/00
Inventor 汤琳欧阳细莲彭博朱旭谭集穗
Owner HUNAN UNIV
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