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Trehalose single-enzyme production monitoring method

A technology of trehalose and trehalose synthase, applied in various fluid ratio control, ratio control, chemical variable control, etc., to achieve accurate and reliable results, reduce monitoring and production costs, and save labor

Active Publication Date: 2017-12-26
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to change the frequent detection of trehalose content manually in the process of single-enzyme production of trehalose into near-infrared automatic detection and automatically control the amount of alkali added according to its changes to achieve the purpose of automatic regulation of production optimization , that is, to provide a method: apply near-infrared real-time detection of trehalose content in the process of single-enzyme production of trehalose, establish a model and correct it by calculating and analyzing the relationship between the change of its content and the change of pH value, and then determine the time and time of adding alkali The amount of alkali is used to stabilize the pH value of the reaction solution so that it is in the optimum pH range with the highest activity of trehalose synthase, so as to achieve the purpose of automatic regulation of production optimization

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1 Monitoring system installation: such as figure 1 As shown, the near-infrared real-time monitoring and control system is installed, including reaction tank (1), throttle valve (2), detection pool (3), optical fiber (4), near-infrared spectrometer (5), computer (6), plus Alkaline pool (7) and throttle valve (8), the throttle valve (2) is set at the outlet of the reaction tank (1), the detection pool (3) is connected to the optical fiber (4) on both sides, the optical fiber (4) and the near-infrared spectrometer (5) connection, the near-infrared spectrometer (5) is connected with the computer (6), and the computer is connected with the alkali-adding pool (7) at the same time, and the alkali-adding pool (7) is connected with the reaction tank (1) through the throttle valve (8).

Embodiment 2

[0016] Example 2 Catalytic reaction: put 90% pure maltose into maltose syrup with a concentration of 20% and put it into the reaction tank (1), set the temperature of the reaction liquid at 34-36°C, and add the Streptomyces griseus ( Streptomyces griseus ) The trehalose synthase of CCTCC AA92094 catalyzes the reaction, and the optimal pH range of the trehalose synthase is 7.2-7.8.

Embodiment 3

[0017] Example 3 Actual pH measurement: Time-sharing sampling was carried out for the two catalytic reactions without pH adjustment and pH adjustment respectively in the reaction process, and the pH value of each sample was measured in real time with a pH meter. The results are shown in Table 2.

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Abstract

The invention discloses a trehalose single-enzyme production monitoring method. The trehalose single-enzyme production monitoring method comprises, mounting a near-infrared real-time monitoring and control system, collecting samples, and associating data such as the pH of every sample which is detected in real time and the content of trehalose of the corresponding sample which is measured through high performance liquid chromatography with near-infrared spectrum information of every sample, and establishing and correcting a quantitative model of change per unit time of trehalose content and pH during a single-enzyme production process; through the corrected quantitative model, performing rapid detection on the change per unit time of trehalose content and pH during the single-enzyme production process, and stabilizing the pH to be within the optimal activity range of trehalose synthase. The trehalose single-enzyme production monitoring method can achieve real-time monitoring of reaction liquid during the single-enzyme production process and meanwhile can automatically control of pH and trehalose content to achieve accurate and reliable results.

Description

technical field [0001] The present invention belongs to an invention about trehalose, more specifically, the present invention relates to a method for monitoring and controlling the production of trehalose by a single enzyme method. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two molecules of glucose connected by α, α-1, 1-glycosidic bonds. The large-scale production methods of trehalose” and “new sugars in the 21st century” have received widespread attention; the production methods of trehalose include natural biological extraction, microbial fermentation, chemical synthesis, genetic engineering and enzymatic conversion; At present, industrial production basically adopts enzymatic conversion method, which is mainly divided into single-enzyme method and double-enzyme method; in the production process of trehalose by single-enzyme method, trehalose synthase converts maltose into trehalose, and the nature of trehalose synthase determines ...

Claims

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Application Information

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IPC IPC(8): G05D21/02G01N21/359G01N21/3577
CPCG01N21/3577G01N21/359G05D21/02
Inventor 谢定郑瑞娜杨倩圆易翠平
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY