Unlock instant, AI-driven research and patent intelligence for your innovation.

The gene encoding mannitol-1-phosphatase in kelp, its protein and its application

A technology of mannitol and phosphatase, applied in the field of genetic engineering, can solve the problems of cumbersome refining process, low recovery rate, limited production process, etc., and achieve the effect of good catalytic effect and high affinity

Active Publication Date: 2020-11-10
OCEAN UNIV OF CHINA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the recovery rate of this method is low, and the refining process is cumbersome, and the source of raw materials is limited by the region and other factors, which greatly restricts this traditional production process.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The gene encoding mannitol-1-phosphatase in kelp, its protein and its application
  • The gene encoding mannitol-1-phosphatase in kelp, its protein and its application
  • The gene encoding mannitol-1-phosphatase in kelp, its protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Acquisition of SjaM1P2 gene in embodiment 1 true kelp

[0042] According to the SjaM1P2 gene sequence shown in SEQ ID NO: 1, the full sequence of the SjaM1P2 gene was synthesized (entrusted to Shanghai Xuguan Biotechnology Development Co., Ltd. to synthesize), and at the same time, BamH I and EcoR I restriction enzyme sites and The corresponding protected bases were used to obtain the SjaM1P2 gene.

Embodiment 2

[0043] Acquisition of SjaM1P2 gene in embodiment 2 true kelp

[0044] Collect kelp, use the Trizol method to extract the total RNA of kelp female gametophytes, use TAKARA's PrimeScriptII 1st Strand cDNA Synthesis kit to use the first-strand cDNA reverse-transcribed from the total RNA of kelp female gametophytes as a template, clone the SjaM1P2 gene by PCR method, and amplify it by PCR Primers were: 5'-gga tcc atggagcag gcaaccgccaacaa-3' and 5'-gaa ttc tta ggc ctt gcc gtg gac cc-3'. The PCR amplification program was: 94°C, 3min; 94°C, 30s, 58°C, 30s, 72°C, 1min, 35 cycles; 72°C, 10min.

[0045] After the PCR products were detected by 1% agarose gel electrophoresis, the gel blocks containing the target bands were cut under ultraviolet light, and the target fragments were recovered using an agarose gel extraction kit, and stored at -20°C.

Embodiment 3

[0046] The vector construction and expression purification of embodiment 3SjaM1P2 gene

[0047] The SjaM1P2 gene synthesized in Example 1 or the SjaM1P2 gene recovered through PCR amplification in Example 2 was connected between the BamH I and EcoR I sites of the pGEX-6P1 vector (purchased from Novagen) to obtain pGEX-6P1 -SjaM1P2 recombinant vector; transform the obtained pGEX-6P1-SjaM1P2 recombinant vector into Escherichia coli E.coli BL21 (DE3) competent cells (purchased from Takara Company), and spread on LB solid culture containing 100 μg / mL ampicillin Cultivate overnight at 37°C on a plate; inoculate positive clones into LB solid medium containing 100 μg / mL ampicillin, culture at 37°C until the OD600 of the bacterial solution is 0.4, add a final concentration of 0.5 mM IPTG, and incubate at 20°C and 160 rpm Cultivate under the induction conditions for 16 hours to induce the expression of the target protein; after the induction, take 2 mL of the bacterial liquid and centr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of genetic engineering and more particularly relates to mannitol-1-phosphatase encoding gene in Saccharina japonica, and a protein and application of the mannitol-1-phosphatase encoding gene. The invention discloses mannitol-1-phosphatase encoding gene SjaM1P2 in Saccharina japonica, and a sequence of the gene SjaM1P2 is shown as in SEQ ID NO: 1. An encoding product of the gene is mannitol-1-phosphatase, an amino acid sequence of the mannitol-1-phosphatase is shown as in SEQ ID NO: 2, and the mannitol-1-phosphatase is active in catalyzing conversion of mannitol-1-phosphoric acid into mannitol. The encoding product of the gene SjaM1P2 disclosed herein is the mannitol-1-phosphatase, and the mannitol-1-phosphatase has great ability to bind with a substrate, has high catalytic efficiency and is applicable to the production of mannitol.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and more specifically relates to a gene encoding mannitol-1-phosphatase in kelp, its protein and application. Background technique [0002] Mannitol is a hexavalent sugar alcohol (C6H14O6), which is isomers with Sorbitol, Iditol and Talitd. Mannitol is white or colorless needle-like crystal or crystalline powder, odorless, sweet, and its sweetness is equivalent to 40%-50% of sucrose. It is stable to dilute acids and alkalis, and is the only polyol that does not absorb moisture. [0003] Mannitol widely exists in bacteria, fungi, protozoa, higher plants and algae. In 1806, Proust isolated mannitol from honeydew tree for the first time. In 1884, Stenhouse discovered mannitol from brown algae, which was also the first crystalline sugar alcohol found in nature. Mannitol is more abundant in edible fungi, lichens and carrots, and also abundant in brown algae. [0004] The various characteristics ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/55C12N9/16C12N1/21C12N1/19C12N5/10C12P7/18
CPCC12N9/16C12P7/18C12Y301/03022
Inventor 刘涛池姗刘翠
Owner OCEAN UNIV OF CHINA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com