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Extraction method of casein phosphopeptide

A technology of casein phosphopeptide and sodium casein phosphate, which is applied in the field of biologically active peptides, can solve the problems of increasing the difficulty and production cost of industrial production in the purification steps, and the complicated process of casein phosphopeptide, so as to reduce the difficulty of preparation and reduce the production cost. Effects of cost, many phosphoserine residues, and high calcium holding capacity

Inactive Publication Date: 2018-01-09
TIANJIN TANGCHAO FOOD IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The process for preparing casein phosphopeptides in Comparative Document 1 and Comparative Document 2 is complex, especially the purification steps increase the difficulty and production cost of industrial production

Method used

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  • Extraction method of casein phosphopeptide

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment one: casein phosphopeptide extraction method, comprises the following steps:

[0031] Step (1): Add 100Kg of water into the enzymolysis tank, stir at 42°C and 50r / min, then add 33.5Kg of sodium casein phosphate into the enzymolysis tank to obtain a suspension of sodium casein phosphate;

[0032] Step (2): adding compound protease for enzymolysis to obtain a casein phosphopeptide solution; the mass ratio of the compound protease is chymotrypsin:flavor protease:papain=1.5:3:2; enzymatic hydrolysis conditions are: adding compound protease The amount is 0.5% of the mass of casein sodium phosphate, the pH value is 6.8, the reaction temperature is 42°C, the stirring speed is 50r / min, and the reaction time is 6h; step (3): the casein phosphopeptide solution is subjected to high-speed centrifugation in sequence and spray drying to obtain powdered casein phosphopeptides with a CPPs content of ≥85%, a molecular weight of 1000-1750Da, no bitter taste, good water solubil...

Embodiment 2

[0033] Embodiment two: casein phosphopeptide extraction method, comprises the following steps:

[0034] Step (1): Add 100Kg of water into the enzymolysis tank, stir at 45°C and 45r / min, then add 66.5Kg of sodium casein phosphate into the enzymolysis tank to obtain a suspension of sodium casein phosphate;

[0035] Step (2): Add compound protease for enzymolysis to obtain casein phosphopeptide solution; the mass ratio of compound protease is chymotrypsin:flavor protease:papain=3:2:1.5; the enzymolysis condition is: add compound protease The amount is 2.5% of the mass of sodium casein phosphate, the pH value is 7, the reaction temperature is 45°C, the stirring speed is 45r / min, and the reaction time is 5h;

[0036] Step (3): The casein phosphopeptide solution is subjected to high-speed centrifugation and spray drying in sequence to obtain powdered casein phosphopeptides with a CPPs content of ≥85%, a molecular weight of 1000-1750Da, no bitterness, good water solubility, and a mas...

Embodiment 3

[0037] Embodiment three: casein phosphopeptide extraction method, comprises the following steps:

[0038] Step (1): Add 100Kg of water into the enzymolysis tank, stir at 48°C and 55r / min, then add 50Kg of sodium casein phosphate into the enzymolysis tank to obtain a suspension of sodium casein phosphate;

[0039] Step (2): Add compound protease for enzymolysis to obtain casein phosphopeptide solution; the mass ratio of compound protease is chymotrypsin:flavor protease:papain=2:1.5:3; enzymolysis conditions are: add compound protease The amount is 5% of the mass of sodium casein phosphate, the pH value is 7.2, the reaction temperature is 48°C, the stirring speed is 55r / min, and the reaction time is 4h;

[0040] Step (3): The casein phosphopeptide solution is subjected to high-speed centrifugation and spray drying in sequence to obtain powdered casein phosphopeptides with a CPPs content of ≥85%, a molecular weight of 1000-1750Da, no bitterness, good water solubility, and a mass ...

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Abstract

The invention discloses an extraction method of casein phosphopeptide. The extraction method comprises the following steps: (1) adding water into an enzymolysis tank, carrying out stirring under the condition that the temperature is 42-48 DEG C and the speed is 45-55 r / min, and adding casein sodium phosphate into the enzymolysis tank, thus obtaining casein sodium phosphate suspension; (2) adding compound protease for enzymolysis, thus obtaining casein phosphopeptide solution, wherein the compound protease is at least three of neutral protease, chymotrypsin, flavourzyme and papain, the enzymolysis conditions are as follows: the adding amount of the compound protease is 0.5-5 % of the mass of casein sodium phosphate, the pH is 6.8-7.2, the reaction temperature is 42-48 DEG C, the stirring speed is 45-55 r / min, and the reaction time is 4-6 h; and (3) carrying out high-speed centrifugal separation on the casein phosphopeptide solution and carrying out spray-drying sequentially, thus obtaining powdered casein phosphopeptide. According to the extraction method provided by the invention, a preparation process is simplified, the preparation difficulty and production cost of industrializedproduction are reduced, and the product casein phosphopeptide has relatively high calcium binding capacity.

Description

technical field [0001] The invention relates to the technical field of bioactive peptides, more specifically, it relates to a casein phosphopeptide extraction method. Background technique [0002] In recent years, many biologically active peptides have been identified through protein digestion. Bioactive peptides are one of the hot spots in animal nutrition research at home and abroad in recent years. Casein Phosphopeptides (CPPs) are natural physiologically active peptides containing phosphoserine clusters obtained from bovine casein as raw material, hydrolyzed by a single or complex protease, and then separated and purified from the hydrolyzed product. CPPs can combine with calcium, iron, zinc, selenium and other divalent mineral ions in the small intestine environment of animals to prevent precipitation and enhance the concentration of soluble minerals in the intestine, thereby promoting the intestinal mucosa to calcium, iron, zinc, selenium, Especially the absorption an...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 吕桂福谭剑云徐伟
Owner TIANJIN TANGCHAO FOOD IND CO LTD
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